Jump to main content
Jump to site search
SCHEDULED MAINTENANCE Close the message box

Maintenance work is planned for Monday 16 August 2021 from 07:00 to 23:59 (BST).

Website performance may be temporarily affected and you may not be able to access some PDFs or images. If this does happen, refreshing your web browser should resolve the issue. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 10, 2015

Quantifying cell-induced matrix deformation in three dimensions based on imaging matrix fibers

Author affiliations

Abstract

During processes such as development and cancer metastasis, cells migrate into three-dimensional fibrous matrices. Previous studies have speculated on the mechanical forces required for migration by observing matrix fiber alignment, densification, and degradation, but these forces remain difficult to quantify. Here we present a new experimental technique to simultaneously measure full-field 3D displacements and structural remodeling of a fibrous matrix, both of which result from cellular forces. We apply this “2-in-1” experimental technique to follow single cells as they invade a physiologically relevant fibrin matrix. We find that cells generate tube-like structures in the matrix by plastically deforming their surroundings, and they re-use these tubes to extend protrusions. Cells generate these tubular structures by applying both pulling and pushing forces.

Graphical abstract: Quantifying cell-induced matrix deformation in three dimensions based on imaging matrix fibers

Supplementary files

Article information


Submitted
17 Jan 2015
Accepted
19 May 2015
First published
19 May 2015

Integr. Biol., 2015,7, 1186-1195
Article type
Paper

Search articles by author

Spotlight

Advertisements