Oxygen governs gonococcal microcolony stability by enhancing the interaction force between type IV pili

Abstract

The formation of small bacterial clusters, called microcolonies, is the first step towards the formation of bacterial biofilms. The human pathogen Neisseria gonorrhoeae requires type IV pili (T4P) for microcolony formation and for surface motility. Here, we investigated the effect of oxygen on the dynamics of microcolony formation. We found that an oxygen concentration exceeding 3 μM is required for formation and maintenance of microcolonies. Depletion of proton motive force triggers microcolony disassembly. Disassembly of microcolonies is actively driven by T4P retraction. Using laser tweezers we showed that under aerobic conditions T4P–T4P interaction forces exceed 50 pN. Under anaerobic conditions T4P–T4P interaction is severely inhibited. We conclude that oxygen is required for gonococcal microcolony formation by enhancing pilus–pilus interaction.