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Themed collection Directed Evolution

13 items
Feature Article

Directed evolution 2.0: improving and deciphering enzyme properties

A KnowVolution: knowledge gaining directed evolution including four phases is proposed in this feature article, which generates improved enzyme variants and molecular understanding.

Graphical abstract: Directed evolution 2.0: improving and deciphering enzyme properties
From the themed collection: Directed Evolution
Feature Article

Cascade catalysis – strategies and challenges en route to preparative synthetic biology

In this feature article recent progress and future perspectives of cascade catalysis combining bio/bio or bio/chemo catalysts are presented.

Graphical abstract: Cascade catalysis – strategies and challenges en route to preparative synthetic biology
From the themed collection: Directed Evolution
Feature Article

Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio- and stereoselective oxidative hydroxylation

Cytochrome P450 enzymes (CYPs) have been used for more than six decades as catalysts for the CH-activating oxidative hydroxylation of organic compounds with formation of added-value products.

Graphical abstract: Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio- and stereoselective oxidative hydroxylation
Communication

Rationally engineered variants of S-adenosylmethionine (SAM) synthase: reduced product inhibition and synthesis of artificial cofactor homologues

Rational redesign of bacterial S-adenosylmethionine-synthase by 3D-modelling and docking led to variants that allow synthesis of methylation cofactor SAM (AdoMet) without product inhibition, and of higher alkyl homologues.

Graphical abstract: Rationally engineered variants of S-adenosylmethionine (SAM) synthase: reduced product inhibition and synthesis of artificial cofactor homologues
From the themed collection: Directed Evolution
Communication

Non-hazardous Baeyer–Villiger oxidation of levulinic acid derivatives: alternative renewable access to 3-hydroxypropionates

Baeyer–Villiger monooxygenases catalyze the energetically challenging oxidation of levulinates (4-oxopentanoates) to 3-hydroxypropionic acid (3-HPA) derivates under ambient conditions, replacing propellant-grade H2O2 with aerial oxygen as the oxidant.

Graphical abstract: Non-hazardous Baeyer–Villiger oxidation of levulinic acid derivatives: alternative renewable access to 3-hydroxypropionates
From the themed collection: Directed Evolution
Communication

A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

A synthetic biology approach enabled the design of a single E. coli cell catalyst co-expressing three enzymes (L-AAD, L- or D-HIC and FDH) for the quantitatively transformation of L-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids.

Graphical abstract: A synthetic biology approach for the transformation of l-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids
From the themed collection: Directed Evolution
Communication

Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase

We report the first recombinant enzyme in E. coli capable of transforming ethylene to ethylene oxide.

Graphical abstract: Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase
From the themed collection: Directed Evolution
Open Access Communication

Compact multi-enzyme pathways in P. pastoris

2A peptides enabling the coordinate expression of nine genes in P. pastoris represent a valuable tool for pathway construction and engineering.

Graphical abstract: Compact multi-enzyme pathways in P. pastoris
From the themed collection: Directed Evolution
Communication

Myoglobin-catalyzed intermolecular carbene N–H insertion with arylamine substrates

Engineered variants of sperm whale myoglobin efficiently catalyze the insertion of α-diazo esters into the N–H bond of arylamines, featuring a combination of high chemoselectivity, elevated turnover numbers, and broad substrate scope.

Graphical abstract: Myoglobin-catalyzed intermolecular carbene N–H insertion with arylamine substrates
From the themed collection: Directed Evolution
Communication

Saturation mutagenesis in selected amino acids to shift Pseudomonas sp. acidic lipase Lip I.3 substrate specificity and activity

Several Pseudomonas sp. CR611 Lip I.3 mutants with overall increased activity and a shift towards longer chain substrates were constructed.

Graphical abstract: Saturation mutagenesis in selected amino acids to shift Pseudomonas sp. acidic lipase Lip I.3 substrate specificity and activity
From the themed collection: Directed Evolution
Communication

Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol

Directed evolution of a P450pyr created I83M/I82T mutant as the first catalyst for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol.

Graphical abstract: Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol
From the themed collection: Directed Evolution
Open Access Communication

A thermostable transketolase evolved for aliphatic aldehyde acceptors

Directed evolution of a thermostable transketolase yields catalysts with significant improvement in activity, enantioselectivity and substrate scope.

Graphical abstract: A thermostable transketolase evolved for aliphatic aldehyde acceptors
From the themed collection: Directed Evolution
Communication

RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

Cell-free transcription–translation systems offer an effective and versatile platform to explore the impact of genetic variations on protein function.

Graphical abstract: RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day
From the themed collection: Directed Evolution
13 items

About this collection

Guest edited by Nicholas Turner and Frances Arnold, this web collection celebrates the current achievements and future perspectives in the excited field of directed evolution

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