Issue 5, 2015

Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol

Abstract

Directed evolution of P450pyr created I83M/I82T mutant for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol, representing the first achievement of this hydroxylation reaction by chemical or enzymatic methods and an unique example of evolving a hydroxylase to switch the substrate acceptance from a hydrophobic to hydrophilic compound.

Graphical abstract: Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol

Supplementary files

Article information

Article type
Communication
Submitted
27 Oct 2014
Accepted
20 Nov 2014
First published
25 Nov 2014

Chem. Commun., 2015,51, 914-917

Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol

Y. Yang, Y. T. Chi, H. H. Toh and Z. Li, Chem. Commun., 2015, 51, 914 DOI: 10.1039/C4CC08479A

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