Bacteriophage based probes for pathogen detection
Rapid and specific detection of pathogenic bacteria is important for the proper treatment, containment and prevention of human, animal and plant diseases.
Single-stranded DNA (ssDNA) production in DNA aptamer generation
Different methods of ssDNA generation following the PCR amplification step in SELEX are reviewed.
Surface enhanced Raman scattering for multiplexed detection
This review focuses on recent advances in the use of surface enhanced Raman scattering (SERS) as an analytical technique for multiplexed detection of biological targets.
Modern analytical techniques in metabolomics analysis
This review highlights the importance and benefit of the role of integrated tools in metabolomic research.
Advances in microfluidics for environmental analysis
We review microfluidic devices for environmental analysis, including recent advances in the technology and the challenges associated with detecting low-abundance analytes in complex sample matrices.
Electrochemical determination of glutathione: a review
The electrochemical detection of glutathione is reviewed with the state of the art highlighted and issues for future research identified.
Novel nanobiotechnological concepts in electrochemical biosensors for the analysis of toxins
The incorporation of novel nanobiotechnological concepts such as aptamers, nanoparticles, nanotubes and magnetic beads, in sensing and transducing strategies is providing with high-performance electrochemical biosensors for toxin detection.
Recent advances in electrochemical sensing for
hydrogen peroxide: a review
Due to the significance of hydrogen peroxide in biological systems and practical applications, the development of efficient electrochemical H2O2 sensors holds a special attraction for researchers. The aim of this review is to summarize the recent advances since 2000 and gain an insight into the materials used in the electrocatalytic sensing of H2O2.
BODIPY based colorimetric
fluorescent probe for selective thiophenol detection: theoretical and experimental studies
A BODIPY-based selective thiophenol probe capable of discriminating aliphatic thiols is reported. The fluorescence off–on effect upon reaction with thiol is elucidated with theoretical calculations. The sensing of thiophenol is associated with a color change from red to yellow and 63-fold enhancement in green fluorescence. Application of the probe for selective thiophenol detection is demonstrated by live cell imaging.
A facile and sensitive detection of pathogenic bacteria using magnetic
nanoparticles and optical nanocrystal probes
A facile and sensitive analytical method for the detection of Salmonella bacteria using antibody-conjugated magnetic nanoparticles and TiO2 nanocrystals is reported.
Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip
Detection of the influenza virus, using LFIA, for amplified DNA.
Quantum dot enabled detection of Escherichia coli using a cell-phone
We report a cell-phone based Escherichia coli detection platform for screening of liquid samples using quantum dots.
Easy dual-mode ambient mass spectrometry with Venturi self-pumping, canned air, disposable parts and voltage-free sonic-spray ionization
An exceptionally simple and easy to assemble dual-mode source for ambient mass spectrometry is described. The Spartan source is fully portable and disposable, provides efficient desorption/ionization and is able to handle both liquid and solid samples.
electrochemical detection of proteins using aptamer-coated gold nanoparticles and surface enzyme reactions
An electrochemical-based immunosensor involving the combined use of DNA aptamer functionalized nanoparticles immobilized onto the electrode surface and a surface enzymatic reaction was developed for the femtomolar detection of protein biomarkers.
Highly specific colorimetric recognition and sensing of sulfide with glutathione-modified
gold nanoparticle probe based on an anion-for-molecule ligand exchange reaction
A gold nanoparticle-based colorimetric sensor for sulfide with remarkably high selectivity was developed, on the basis of a unique anion-for-molecule ligand exchange reaction.
gold nanoparticles for detection of Cu2+ with good sensitivity and selectivity
Stable and highly fluorescent gold nanoparticles were developed to detect copper ions in aqueous solution with good sensitivity and selectivity.
Ultrasensitive aptamer biosensor for arsenic(III) detection in aqueous solution based on
surfactant-induced aggregation of gold nanoparticles
A novel and effective procedure for AuNPs aggregation via the special interactions among aptamer, target and cationic surfactant was developed for As(III) detection.
fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells
We present applications of an off–on fluorescent probe PyBor for peroxynitrite detection in aqueous solution and living cells.
DNA-templated silver nanoclusters–graphene oxide nanohybrid materials: a platform for label-free and sensitive fluorescence turn-on detection of multiple nucleic acid targets
We develop an efficient method for multiple DNA detection by exploring silver nanoclusters (AgNCs)–graphene oxide (GO) nanohybrid materials.
Highly selective and sensitive detection of Cu2+ with
lysine enhancing bovine serum albumin modified-carbon dots fluorescent probe
Lysine acts to decrease surface defects and enhance the fluorescence intensity of a bovine serum albumin modified-carbon dot probe used to detect Cu2+ in hair and tap water.
silver nanoparticles as ion and pH sensors in aqueous solution
Silver nanoparticles capped with mercaptoacetic acid and 2-aminoethanethiol short-chain alkanethiols were prepared by a one-step method in aqueous solution for monitoring pH and a range of heavy metal ions.
Sensitive sandwich ELISA based on a
gold nanoparticle layer for cancer detection
This simple and cost-effective gold nanoparticle layer (GNPL)-based sandwich ELISA holds promise in clinical applications.
Protein-gold nanoclusters for identification of amino acids by metal ions modulated ratiometric fluorescence
Metal ion-modulated bovine serum albumin-Au nanoclusters were used to construct a sensor array for identification of amino acids by the fluorescent fingerprint.
Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices
Surface-sensitive electrical detection using chelator-modified field-effect transistor devices designed to capture pyrophosphate generated by polymerase reactions on surface-immobilized DNA colonies.
gold nanoparticle-based immunochromatographic assay: The influence of nanoparticulate size
The influence of nanoparticulate size on a gold nanoparticle-based immunochromatographic assay in biomedicine is presented.
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The top 25 most read articles of 2012 in Analyst