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Issue 5, 2012
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A gold nanoparticle-based immunochromatographic assay: The influence of nanoparticulate size

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Abstract

Four different sized gold nanoparticles (14 nm, 16 nm, 35 nm and 38 nm) were prepared to conjugate an antibody for a gold nanoparticle-based immunochromatographic assay which has many applications in both basic research and clinical diagnosis. This study focuses on the conjugation efficiency of the antibody with different sized gold nanoparticles. The effect of factors such as pH value and concentration of antibody has been quantificationally discussed using spectra methods after adding 1 wt% NaCl which induced gold nanoparticle aggregation. It was found that different sized gold nanoparticles had different conjugation efficiencies under different pH values and concentrations of antibody. Among the four sized gold nanoparticles, the 16 nm gold nanoparticles have the minimum requirement for antibody concentrations to avoid aggregation comparing to other sized gold nanoparticles but are less sensitive for detecting the real sample compared to the 38 nm gold nanoparticles. Consequently, different sized gold nanoparticles should be labeled with antibody under optimal pH value and optimal concentrations of antibody. It will be helpful for the application of antibody-labeled gold nanoparticles in the fields of clinic diagnosis, environmental analysis and so on in future.

Graphical abstract: A gold nanoparticle-based immunochromatographic assay: The influence of nanoparticulate size

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Publication details

The article was received on 12 Sep 2011, accepted on 24 Nov 2011 and first published on 22 Dec 2011


Article type: Paper
DOI: 10.1039/C2AN15844B
Citation: Analyst, 2012,137, 1174-1181
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    A gold nanoparticle-based immunochromatographic assay: The influence of nanoparticulate size

    S. Lou, J. Ye, K. Li and A. Wu, Analyst, 2012, 137, 1174
    DOI: 10.1039/C2AN15844B

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