Issue 15, 2012
From the journal:

Analyst

Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip

Naoki Nagatani,*a   Keiichiro Yamanaka,b   Hiromi Ushijima,c   Ritsuko Koketsu,d   Tadahiro Sasaki,d   Kazuyoshi Ikuta,d   Masato Saito,b   Toshiro Miyaharaa  and  Eiichi Tamiyab  

* Corresponding authors

a Department of Applied Chemistry, Graduate School of Engineering, Okayama University of Science, 1-1, Ridai-cho, Kita-ku, Okayama-shi 700-0005, Japan
E-mail: nagatani@dac.ous.ac.jp
Fax: +81-86-252-6891
Tel: +81-86-256-9744

b Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita-shi, Osaka 565-0871, Japan

c Biodevicetechnology Ltd, 2-13 Asahidai, Nomi-City, Ishikawa 923-1211, Japan

d Department of Virology, Research Institute for Microbial Diseases (BIKEN), Osaka University, 2-1, Yamadaoka, Suita-shi, Osaka 565-0871, Japan

Abstract

Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15–20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min.

Graphical abstract: Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip

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Article information

DOI
https://doi.org/10.1039/C2AN16294F
Article type
Communication
Submitted
23 Dec 2011
Accepted
07 Feb 2012
First published
22 Feb 2012

Analyst, 2012,137, 3422-3426

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Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip

N. Nagatani, K. Yamanaka, H. Ushijima, R. Koketsu, T. Sasaki, K. Ikuta, M. Saito, T. Miyahara and E. Tamiya, Analyst, 2012, 137, 3422 DOI: 10.1039/C2AN16294F

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