Bin
Yao
a,
Praveen
Kolla
b,
Ranjit
Koodali
c,
Selvaratn
Balaranjan
c,
Sunav
Shrestha
c and
Alevtina
Smirnova
*ab
aMaterials Engineering and Science Program, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA. E-mail: Alevtina.Smirnova@sdsmt.edu
bChemistry and Applied Biological Sciences Department, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA
cChemistry Department, University of South Dakota, Vermillion, SD 57069, USA
First published on 10th July 2017
Being a major byproduct of pulp and paper industry, lignin has attracted attention as a source of high-value organic chemicals, e.g. phenolic monomers that can be produced by lignin enzymatic treatment. In this study, the alkali lignin was treated by the laccase of Trametes versicolor (LTV) and laccase of Myceliophthora thermophila (LMT) laccases with or without natural mediator methyl syringate (MS). After treatment, the lignin pore volume has been increased by 66% and 167% for LTV–MS and LMT–MS systems, respectively. The mass balance analysis confirms that the 5 wt% LTV–5 wt% MS (LTV–MS (5, 5)) system produces 40 wt% more of the organic compounds than the 5 wt% LMT–5 wt% MS (LMT–MS (5, 5)) system, thus demonstrating higher efficiency of the LTV–MS toward lignin decomposition. The gas chromatography-mass spectroscopy (GCMS) analysis indicates that after lignin treatment with LTV–MS more phenolic products are produced in comparison to LMT–MS, among them 3-hydroxy-4-methoxy-benzaldehyde has the highest relative yield of 34.7 and 23.8 wt% for LTV–MS and LMT–MS systems, respectively. Pyrolysis-GCMS (pyr-GCMS) of the solid-state lignin residue after its treatment with laccase–mediator system (LMS) confirms significant enrichment of the solid-state lignin surface with phenolic groups. The total organic carbon (TOC) analysis shows that 38.76 mg L−1 of the organic carbon is produced by the LTV–MS (5, 5) system. A decrease in the decomposition temperature by 4 °C for the lignin sample treated by the LTV–MS (in comparison to LMT–MS) obtained from the thermogravimetric (TGA) analysis confirms superior performance of the LTV–MS vs. LMT–MS system. A comparative study of the enzymatically active systems with two laccases and a natural mediator applied to the alkali lignin has been performed. This study highlights an important role of the laccase in combination with a mediator methyl syringate for production of high-value phenolic monomers.
Microbes are known to naturally decompose lignin.6 Among them, white rot fungi is known as the most efficient microbe for lignin degradation that produces three peroxidases7 and laccase enzymes.8 The copper-containing laccase (p-diphenol oxygen oxidoreductase, EC 1.10.3.2) is able to catalyze the one-electron oxidation of phenolic substrates9 that supply electrons to the laccase molecule for the four electron reduction of oxygen to water.10 This process takes place at the four copper centers which contain three kinds of copper, such as T1, T2 and T3.11 One of them, Type I (T1) Cu, plays the dominant role in the lignin oxidation processes and shows a characteristic transfer absorption band of sulfur to Cu(II) at 610 nm.12 However, besides well-known catalytic activity of laccases toward oxidation of the phenolic groups, they cannot oxidize lignin completely.5 One of the reasons is in large size of the laccase molecules, that cannot penetrate lignin pores and oxidize its internal surfaces.13 The second reason is in the redox potential (0.4–0.8 V) of laccases which is not sufficiently high for oxidation of the non-phenolic groups of lignin.14 Consequently, a mediator as an electron carrier, assisting laccase in lignin depolymerization, is considered as a key player in enhancing the system efficiency15 toward synthesis of phenolic compounds.16
Most mediators tested earlier for lignin degradation are artificial compounds based on nitrogen heterocycles.17 For example, the artificial mediator 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)18 was used in combination with laccase for evaluation of its activity in presence of two lignin model compounds (3,4-dimethoxyphenyl)-2-phenylethanediol and 1-(3,4-dimethoxy-phenyl)-2-(2-methoxyphenoxy)-propane-1,3-diol.19 The LMS with artificial 5,5′-hydrazotetrazole (HBT) mediator was investigated for paper pulp delignification and ethanol production from lignocellulose.20 Moreover, the LMS system based on Pycnoporus cinnabarinus laccase and HBT mediator was applied to a delignification bleaching processes in the pulp and paper industry.21
At present, significant attention is paid to the natural mediators which in comparison to the artificial mediators are economically viable and environmentally friendly.22 Among them, methyl syringate as a derivative of guaiacol is considered as a potential natural mediator for lignin degradation.23
Though numerous articles have been published in regard to the enzymatic degradation of lignin by natural24 and artificial16 mediators, this work has a novelty in using two different laccases and detailed analysis of their decomposition products produced in presence of natural mediator methyl syringate.23 For comparison of the lignin decomposition products, two different laccases, specifically LMT25 and LTV26 with the corresponding oxidation potentials of 0.48 V and 0.78 V have been studied. The difference between the two laccases was in their enzymatic activity pertinent to different temperature ranges and pH values, as well as in their electrochemical behavior that was investigated in our previous studies.27,28 Recently, the laccase from Myceliophthora thermophila in combination with methyl syringate was tested for removal of lignin and was demonstrated to enhance saccharification from Eucalyptus feedstock.29
The purpose of this study is in comparison of the ratios of the laccase and mediator and evaluation of the nature of the laccase that can assist in synthesis of the high-value phenolic products from lignin. Specifically, the analysis of the lignin decomposition products in presence of two laccases and a natural mediator methyl syringate is considered. In this study, the alkali lignin degradation efficiency and the reaction products, such as solid and organic liquid phases have been characterized by different techniques, such as SEM, BET, TGA, TOC, GCMS, and pyr-GCMS.
The lignin samples were treated in buffer solutions for 72 h at 40 °C and 70 °C for LTV and LMT, respectively. The results of the lignin decomposition in presence of the LMS system were compared with a baseline experiments when the lignin sample was treated under the same reaction conditions, but without mediator.
After treatment, the samples were filtered using a Büchner funnel and washed with 10 mL of dichloromethane (DCM). The pH values of the liquid fractions, controlled by a pH meter, were adjusted to ∼2.5 by addition of 1 wt% HCl. The liquid organic fractions were extracted by DCM (3 times, 20 mL each). For evaluation of the mass balance, the organic liquid phases from three extractions were combined and dried completely by using a rotary evaporator. For GCMS analysis, 1.0 mL DCM and 10 μL of the internal standard were added to each of the dried samples. For pyrolysis-GCMS (pyr-GCMS), the solid lignin residues were dried in the oven at 50 °C for 24 h.
The distinction between the morphology of the LTV–MS and LMT–MS treated samples of lignin was confirmed by the comparative BET analysis (Fig. 2, Table 1). The recorded type IV nitrogen adsorption–desorption isotherms (Fig. 2a) is an indication of an interconnected porous mesostructured. The observed hysteresis is due to the nitrogen capillary-condensation phenomenon which is typical for mesoporous materials.
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Fig. 2 Comparison of the nitrogen adsorption–desorption isotherms (77 K) of the alkali lignin (a) and the pore size distribution (b) for lignin before and after treatment with LMT–MS and LTV–MS. |
Alkali lignin | Specific surface area (m2 g−1) | Pore volume (cc g−1) |
---|---|---|
Before treatment | 4.9 | 0.006 |
After LMT–MS treatment | 6.1 | 0.01 |
After LTV–MS treatment | 9.8 | 0.016 |
As a result of enzymatic treatment, the specific surface area (SSA) of the lignin was improved to up to 1.2 and 2 times after its treatment with LMT–MS and LTV–MS system, respectively. Compared to the original lignin, the pore volume of the lignin treated with LMT–MS and LVT–MS was 1.6× and 2.6× higher corresponding to the respective ∼66% and ∼167% increase in pore volume.
A comparison of the Pore-Size-Distribution (PSD) for the alkali lignin treated with LMS systems (Fig. 2b) indicates that the pore volume is enhanced due to formation of new mesopores. The results demonstrate that both LMS systems are catalytically active toward lignin degradation, especially the LTV–MS one. This observation is in agreement with the SEM analysis confirming that both LMS systems are involved in the process of lignin degradation.
Name of the compounda | RTb | m/z | Control sample |
---|---|---|---|
a The names of the compounds are based on the identification using the MS NIST library. b Retention time. | |||
Phenol, 2-methoxy- | 8.88 | 124.00 | |
4-Allyl-2-methoxyphenol | 12.85 | 164.08 | |
3-Hydroxy-4-methoxy-benzaldehyde | 13.48 | 152.00 | ✓ |
(E)-2-Methoxy-4-(prop-1-en-1-yl)phenol | 14.13 | 164.08 | |
Phenol, 2-methoxy-4-propyl- | 14.16 | 166.10 | |
1-(4-Hydroxy-3-methoxyphenyl)ethanone | 14.63 | 166.06 | ✓ |
2-Propanone, 1-(4-hydroxy-3-methoxyphenyl)- | 15.08 | 180.08 | |
2,6-Dimethoxybenzoquinone | 15.46 | 168.04 | |
Benzeneacetic acid, 4-hydroxy-3-methoxy-, methyl ester | 17.39 | 196.07 | |
4-(2-Hydroxyethyl)-2-methoxyphenol | 19.10 | 168.00 | |
2-(4-Hydroxy-3-methoxyphenyl)acetic acid | 28.20 | 182.00 |
Name of the compounda | RTb | m/z | Control sample |
---|---|---|---|
a The names of the compounds are based on the identification using the MS NIST library. b Retention time. | |||
Phenol, 2-methoxy- | 8.88 | 124.00 | |
3-Hydroxy-4-methoxy-benzaldehyde | 13.48 | 152.00 | ✓ |
1-(4-Hydroxy-3-methoxyphenyl)ethanone | 14.63 | 166.06 | |
4-(2-Hydroxyethyl)-2-methoxyphenol | 19.10 | 168.00 | |
2-(4-Hydroxy-3-methoxyphenyl)acetic acid | 28.20 | 182.00 |
The results of the mass balance (Table 4) demonstrate that the yields of the extractable organics in the aqueous/organic DCM extract increases with the concentrations of laccase and mediator. For example, in case of LTV–MS this increase corresponds to 4.6, 8.4 and 12.3 wt% for LTV–MS (1, 1), LTV–MS (3, 3), and LTV–MS (5, 5) system, respectively.
LTV | LMT | |||||
---|---|---|---|---|---|---|
Laccase (wt%) | 1 | 3 | 5 | 1 | 3 | 5 |
Yields of extracted organic phase vs. lignin (wt%) | 0.4 | 1.4 | 2.1 | 0.3 | 0.5 | 1.3 |
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LTV–MS ratio (wt%) | LMT–MS ratio (wt%) | |||||
Laccase–mediator ratio (wt%) | 1–1 | 3–3 | 5–5 | 1–1 | 3–3 | 5–5 |
Yields of extracted organic phase vs. lignin (wt%) | 4.6 | 8.4 | 12.3 | 3.5 | 5.4 | 8.6 |
The highest amount of the phenolic products was produced from 50 g L−1 of lignin with LTV–MS (5, 5) after the DCM extraction and was 10.2 wt% higher than that for the control sample. The mass balance results also show that for the LTV without MS, only 2.1 wt% of organic compound was extracted by DCM compared to 12.3 wt% when the MS was also present. This result demonstrates an important role of the methyl syringate as a natural mediator in the enzymatic lignin degradation.
The GC-MS chemical analysis of the lignin decomposition products obtained with LTV–MS reveals 11 phenolic monomers, with the four major products being 2-methoxyphenol, 1-(4-hydroxy-3-methoxyphenyl)ethan-1-one, 4-(2-hydroxyethyl)-2-methoxy-phenol, phenol, and 4-methylbenzaldehyde (Table 2). In the control sample, only 1-(4-hydroxy-3-methoxyphenyl)ethanone and 3-hydroxy-4-methoxy-benzaldehyde have been detected. All the detected compounds are in agreement with the internal standard and the NIST library.
In comparison to the LTV–MS, the LMT–MS system produced only five phenolic compounds (Table 3) which is expected from lower enzymatic activity of LMT (2.8 U per mg) vs. LVT (12.9 U per mg). This result of lower LMT enzymatic activity is in correlation with the BET data (Fig. 2).
It is necessary to note, that the relative yields of each of the identified phenolic compounds (Tables 2 and 3) increase with the increased weight percent of the laccase–mediator system. The highest yield was observed for the top concentration of the MS mediator (5 wt%) (Fig. 3).
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Fig. 3 GCMS analysis data of the products formed after lignin treatment with LTV–MS (5, 5) and LMT–MS (5, 5) in comparison to LMT (5 wt%) and LTV (5 wt%) control samples. |
Among all the identified phenolic compounds for both laccase–mediator systems and the control samples, the highest relative yield is obtained for 3-hydroxy-4-methoxy-benzaldehyde. Specifically, the LTV–MS and LMT–MS produced 34.7% and 23.8% of the 3-hydroxy-4-methoxy-benzaldehyde, respectively, which is significantly higher in comparison to both LTV (9.3%) and LMT (5.4%) laccase samples without the MS mediator.
The mass balance (Table 4) indicates that the amount of the organic phase in the DCM extract after lignin enzymatic decomposition in presence of LMT–MS increases from 3.5 to 8.6 wt% with the corresponding increase in the concentration of LMT–MS from 1 to 5 wt%. This result demonstrates that the amount of organic compound in DCM phase follows the concentration of both MS mediator and LMT. In case of the LMT without MS, the yield of DCM extractable organics was only 1.3 wt% compared to 8.6 wt% when the LMT–MS was present. In comparison to LMT–MS, the second system showed the highest amount of phenolic compounds produced from the mixture of lignin (50 g L−1) with LTV–MS (5, 5) system. Specifically, 12.3 wt% of phenolic compounds has been produced which is significantly higher than the corresponding value for the control sample without the MS mediator (2.1 wt%).
The chemical analysis of the major lignin depolymerization products confirms that all of them are phenolic monomers (Fig. 3). However, depending on the type of laccase, different number and the amount of the phenolic monomers was produced. Among the identified phenolic derivatives, the highest relative yields are observed for the LTV–MS system. Among the four detected products, the 3-hydroxy-4-methoxy-benzaldehyde was found to be the major compound.
Other minor products are 2-(4-hydroxy-3-methoxyphenyl)acetic acid, 4-(2-hydroxyethyl)-2-methoxyphenol, and 4-hydroxy-benzaldehyde. Among them only 3-hydroxy-4-methoxy-benzaldehyde is observed in the control sample of LMT–MS system.
These results indicate that two laccases (LTV and LMT) have different effects on the number and relative yields of the phenolic monomers. Comparison of the BET and GC-MS results show that LTV has higher efficiency for lignin depolymerization than LMT. In future studies, higher concentration of both laccase and the mediator will be used to obtain higher amount of phenolic compounds.
After LTV–MS treatment, the lignin sample has the same start-up thermal decomposition temperature (350 °C), however, 80% decomposition is reached at even lower temperature (395 °C) than for the LMT–MS system (412 °C) (Fig. 5b). This observation is in agreement with the BET, GCMS, and TOC results and confirms that LTV–MS has higher catalytic activity toward lignin degradation in comparison to LMT–MS. Higher degree of lignin enzymatic decomposition leads to a lower thermal decomposition temperature of the solid residue. This decomposition temperature is almost 110 °C higher than the one reported earlier for kraft lignin.32 Since the scan rate, lignin type, and the onset of the decomposition temperature are the same, an assumption was made that these two kinds of kraft lignin have different rigidity at molecular level caused by variation of the synthesis conditions.
An increase in pore volumes and the surface areas of the lignin samples after treatment with LTV–MS and LMT–MS was in correlation with activities of the laccases, being the highest for the LTV.
The GC-MS analysis demonstrates that LTV–MS produces higher amount of 3-hydroxy-4-methoxy-benzaldehyde, 2-(4-hydroxy-3-methoxyphenyl)acetic acid, and 4-(2-hydroxyethyl)-2-methoxyphenol than LMT–MS. The highest amount of organic carbon (38.76 mg L−1) was produced by the LTV–MS (5, 5) emphasizing the important role of the methyl syringe concentration. In order to increase the amount of the phenolic products and the overall system efficiency, more efforts should be committed toward searching for new and more enzymatically active catalytic systems.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c7se00209b |
This journal is © The Royal Society of Chemistry 2017 |