Craig
McDonald
* and
David
McGloin
SUPA, Division of Physics, University of Dundee, Nethergate, Dundee, Scotland. E-mail: cymcdonald@dundee.ac.uk
First published on 17th June 2015
We propose and demonstrate a low-cost optical micromanipulation system that makes use of simple microfabricated components coupled to a smartphone camera for imaging. Layering hanging droplets of polydimethylsiloxane (PDMS) on microscope coverslips, and curing with a 100 W bulb, creates lenses capable of optical trapping. Optically trapped 3.93 μm silica beads were imaged with a second hanging droplet lens, doped with 1400 μg mL−1 Sudan II dye. Through doping, a lens with an integrated long-pass filter that effectively blocks the 532 nm trapping light was produced. Illumination was provided by shining a lamp into polystyrene foam packaging, perpendicular to the imaging path, producing a diffuse light source. We observed two dimensional trapping and report a Q value of ∼8.9 × 10−3. The techniques here are an addition to the growing body of work on low cost and adaptable microfluidics.
The development of elastomeric optics using polydimethylsiloxane (PDMS)4 has provided a high quality, low-cost, alternative to many optical components. PDMS has a high transparency (>95%) within the visible wavelength range and a high refractive index (n = 1.47–1.55), making it an ideal material to fashion lenses from. The low moulding temperature (<100 °C) of PDMS negates the need for specialised high temperature equipment, making it easy to work with in the lab.4 Elastomeric optics have found use in an increasing number of novel applications such as a rubber microscope,5 biologically inspired wide field lenses6 and a low-cost digital dermascope, which makes use of a mobile phone with added lenses fabricated from hanging droplets of PDMS.7
With the unprecedented growth of the mobile phone market, low-cost miniature microscopes that exploit the high quality digital camera of many mobile phones have been developed.8–11 Much recent work in the lab on a chip field makes use of smartphone sensing capabilities.12
Liquid droplets can be used to magnify small objects.13,14 A hanging droplet is formed when the interfacial energies (liquid, air and solid surface) and gravity reach equilibrium.14,15 By repeatedly adding and curing a hanging PDMS droplet, Lee et al. formed low-cost elastomer lenses which were able to resolve structures as small as 4 μm.7
In this paper, we present a low-cost and low-tech method of creating PDMS droplet lenses and use these droplet lenses as the basis of a basic optical trap. We use a second droplet lens, doped with Sudan II dye, and a commercial smartphone to image optically trapped 3.93 μm silica beads. Doping of PDMS with Sudan II dye, prior to moulding, produces a long pass filter with negligible autofluorescence.16 Integration of the imaging lens and long pass filter allowed for a more compact system, while still effectively blocking the 532 nm trapping light, preventing saturation of the phone camera. Illumination was provided by shining a lamp into polystyrene foam packaging, perpendicular to the imaging path, producing a diffuse light source that was sufficient for resolving the silica beads. At all stages of fabrication of the system we present methods which make use of materials that are often discarded or overlooked in the lab, or are easily attainable for a low cost. Neglecting the cost of a laser, which could be replaced by a laser pointer or other low cost alternative, our system can be constructed for approximately £23.23, a full cost breakdown of all components required is available in the ESI.† We also present measurements of the trapping ability and efficiency of a droplet lens based optical manipulation system.
Our work fits in very well with the burgeoning field of microfluidics for developing world, real world, applications17 and should integrate straightforwardly with existing optofluidic devices and urban sensing systems.
An anglepoise lamp was used to lower a 100 W incandescent light bulb to ∼20 mm above the microscope slide, to provide heat for the curing step, while the tin foil reflected the heat back towards the droplet lens. Temperatures in excess of 100 °C could be reached, with temperature crudely controlled by raising and lowering the light bulb. Droplet lenses were left in the “oven” until set, which took up to 15 minutes, at temperatures ranging from 70–80 °C. In order to shorten the focal length and increase the numerical aperture (NA) of the lens, up to four droplets were layered one on top of each other, with each droplet cured before the next was applied. Due to the maximum surface tension that the droplet can hold before falling, the eventual droplet shape at the apex of the lens resembles that of a parabola, with a curvature that increases with layer number. The fabricated trapping lens is shown in Fig. 1(d) and cost approximately £0.06 per lens, including the microscope cover glass used as the support layer.‡
The length of the droplet lens (L) was found to vary, with typical measured values of 8.48 ± 0.19 mm and 7.38 ± 0.15 mm. However, the diameter of the entrance pupil (D) and back aperture (BA) was found to remain roughly constant, with average values of, respectively, 2.62 ± 0.27 mm and 6.1 ± 0.2 mm. This led to a variation in measured focal lengths of different lenses, with used trapping lenses having a focal length 0.9 ± 0.21 mm and 1.0 ± 0.26 mm. The effective NA of these lenses was estimated as 0.70 ± 0.17 and 0.68 ± 0.18.
Illumination of the sample was achieved by shining a lamp into polystyrene foam packaging§ perpendicular to the imaging path, Fig. 2. This produced diffuse light, which was sufficient to illuminate the sample without saturating the camera. Images presented in this paper were achieved through illumination with a Thorlabs OSL1-EC fibre illuminator as it was convenient. We found that stray laser light was reflecting from the foam packaging and illuminating the sample with scattered laser light and reducing the image contrast. To counteract this effect we made a PDMS filter, consisting of a single droplet of doped PDMS on a microscope coverslip and cured in a non-inverted geometry. This droplet filter was placed in the illumination pathway, preventing the scatter from degrading the image quality.
Fig. 3 (a) Airy disk produced by PDMS droplet lens, imaged with Mitutoyo 0.55 NA 100× long working distance objective. (b) Line profile through centre of Airy disk. |
A suspension of 3.93 μm diameter silica beads in deionised water, between two microscope coverslips separated by a vinyl spacer, was used as the trapping sample. The particle size was chosen as previous studies7 have shown the smallest resolvable structure with droplet lenses to be 4 μm. Fig. 4 shows a 3.93 μm silica bead which has been trapped using a PDMS droplet lens. We found that the minimum power to trap these beads was 34.0 ± 0.4 mW, in the focal spot. Particle height was observed to change as a function of power, indicating two dimensional trapping.
Fig. 4 3.93 μm silica bead (in focus), optically trapped in two dimensions with a clear PDMS droplet lens, among untrapped, and therefore out of focus, beads. Imaged with Mitutoyo 0.55 NA 100× long working distance objective. A video of the trapped bead is available in the ESI.† |
In order to examine the ability of the droplet based trap to function as a more robust research grade instrument, a simple measure of the transverse Q value18 of the trapping spot was made. The simplest method to find the transverse Q value is to laterally displace the trapped sphere at increasing velocity until it falls from the trap, which is equivalent to the sphere remaining stationary while the surrounding fluid is moved. The applied drag force at the point of escape is equal to the trapping force of the optical manipulation system. Moving the sample with increasing velocity increases the drag force acting on the acting on the sphere, given by Stokes' law. Making use of the Stokes' drag technique, and the expression F = Q(nmP/c), where F and P are, respectively, the trapping force and power, nm is the suspending medium's refractive index and c being the speed of light, the trapping efficiency of the system was determined. 3.93 μm beads were trapped with 58.48 ± 0.08 mW of laser power and a Q value of 0.0089 ± 0.0001 was measured.
The low Q value indicates that quantitative measurements, or experiments which require high optical forces, would not be suited to our low cost, droplet lens based optical manipulation system. However, qualitative measurements and trial experiments could be easily performed.
The laser was turned on and power slowly increased, while the smartphone camera was monitored for any sign of laser light transmitted through the filter lens. Trap power was increased until optical trapping was observed, Fig. 5. Trap power was further increased to 112.2 ± 0.2 mW, more than 3 times the minimum trapping power required, in order to test the filtering ability of the doped droplet lens. At this power, the trapping light started to be visible through the doped PDMS droplet lens.
Through the addition of Sudan II dye, an imaging lens with an integrated long pass filter suitable for fluorescence detection was created. Integration with other microfluidic systems to create simple optofluidic19 devices should be straightforward due to much of the system being made from PDMS. This work, therefore, contributes to the burgeoning field of disposable, point-of-care diagnostic devices by allowing for the simple incorporation of optical trapping, fluorescence microscopy techniques and microfluidics.
An obvious extension for our system is to build it with something like lego, or to 3d print the microscope structure. There are printers that will print/cure polymers, and our thinking is that our methodology is a precursor for the full 3d printing of an optical trapping system, this would be much better engineered than our device, and therefore would be much higher precision, all incorporated onto the camera of a mobile phone. This seems perfectly plausible, with the growing interest in 3d printing of lab equipment, with particular reference to the developing world, as indicated in the recent PLoS Biology paper by Baden et al.20 We also suggest that the device, once developed into a compact form would open up field studies of aerosols, part of the development of compact, mobile, urban sensors for airborne aerosols.21 Our initial development appears to have a number of future developmental options.
Footnotes |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c5ra11431d |
‡ It is assumed that the end user is able to supply tin foil and a lightbulb. |
§ Illumination can be provided from any sufficiently bright lamp – bike lamps, torches and camera flash from mobile phone were all successfully tested. If discarded polystyrene foam packaging cannot be found, it can be purchased for approximately £0.02 per use. |
¶ It is assumed that a system developer would have access to a smartphone, with the vast majority of models suitable for use with our system. |
This journal is © The Royal Society of Chemistry 2015 |