Themed collection Most impactful Chemical Biology articles of 2018

We review pharmacological chaperones used in lysosomal storage diseases, emphasizing medicinal chemistry approaches and mechanisms of action.

Using machine learning, targets were identified for β-lapachone.

By establishing a chemical labeling method in combination with liquid chromatography-mass spectrometry analysis, we reported the widespread existence of various modified nucleoside triphosphates in eukaryotes.

A convenient method to bi-terminally modify proteins using recombinant masking groups reveals that H2A.X ubiquitylation opens chromatin during DNA repair.

We developed a novel strategy for enhanced radiation therapy based on a mitochondria targeted titanium dioxide-gold nanoradiosensitizer.

Two AIEgens are designed for tracking mitochondria and lysosomes with different emission colors using single wavelength excitation to visualize the mitophagy process.

Cu(I) complexes do achieve azide–alkyne annulations of freely difusible small molecules inside mammalian cells.

Arylboronic acid-modified antisense oligonucleotides enable hydrogen peroxide induced gene silencing in mammalian cells.

A PNA-templated peptide ligation reaction has been developed between selenocystine and selenoesters. The methodology was used for the sequence specific detection of miRNA at low concentrations.

Newly described NMR experimental approaches can provide valuable structural details and a complementary means of structure verification.

Peptides with various C-terminal functionalization, including peptides and dendrimers, were prepared via SPPS and an efficient on-resin modification.