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Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

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Abstract

The development of a rapid and chemoselective selenocystine–selenoester peptide ligation that operates at nanomolar reactant concentrations has been developed by utilising PNA templation. Kinetic analysis of the templated peptide ligation revealed that the selenocystine–selenoester reaction was 10 times faster than traditional native chemical ligation at cysteine and to our knowledge is the fastest templated ligation reaction reported to date. The efficiency and operational simplicity of this technology is highlighted through the formation of hairpin molecular architectures and in a novel paper-based lateral flow assay for the rapid and sequence specific detection of oligonucleotides, including miRNA in cell lysates.

Graphical abstract: Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

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Publication details

The article was received on 20 Jun 2017, accepted on 21 Nov 2017 and first published on 22 Nov 2017


Article type: Edge Article
DOI: 10.1039/C7SC02736B
Citation: Chem. Sci., 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

    J. Sayers, R. J. Payne and N. Winssinger, Chem. Sci., 2018, Advance Article , DOI: 10.1039/C7SC02736B

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