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Themed collection Biocatalysis: Natural and biologically inspired synthetic enzymes

16 items
Perspective

Evolution inspired engineering of antibiotic biosynthesis enzymes

Chimeragenesis is an effective tool to probe the structure/function relationships of proteins without high-throughput screening systems. Here the proof-of-principle is presented with three pairs of proteins.

Graphical abstract: Evolution inspired engineering of antibiotic biosynthesis enzymes
Review Article

Immobilized enzymes: understanding enzyme – surface interactions at the molecular level

Interactions between immobilized enzymes and supporting surfaces are complex and context-dependent and can significantly alter enzyme structure, stability and activity.

Graphical abstract: Immobilized enzymes: understanding enzyme – surface interactions at the molecular level
Review Article

A decade of DNA-hybrid catalysis: from innovation to comprehension

Since the pioneering work of Roelfes and Feringa in the field of DNA-based asymmetric catalysis, the unique chirality of oligonucleotides has allowed the development of a variety of asymmetric synthetic transformations. This review offers a complete overview of the field.

Graphical abstract: A decade of DNA-hybrid catalysis: from innovation to comprehension
Review Article

Aerobic catalytic systems inspired by copper amine oxidases: recent developments and synthetic applications

Recently, chemists have developed aerobic quinone-based catalytic systems in order to reproduce enzymatic activity and selectivity of copper amine oxidases but also to expand the scope of amine substrates.

Graphical abstract: Aerobic catalytic systems inspired by copper amine oxidases: recent developments and synthetic applications
Review Article

Spatial organization of multi-enzyme biocatalytic cascades

Multi-enzyme cascades provide a wealth of valuable chemicals. Efficiency of reaction schemes can be improved by spatial organization of biocatalysts. This review will highlight various methods of spatial organization of biocatalysts: fusion, immobilization, scaffolding and encapsulation.

Graphical abstract: Spatial organization of multi-enzyme biocatalytic cascades
Review Article

Post-translational modifications involved in the biosynthesis of thiopeptide antibiotics

Thiopeptide antibiotics are generated from ribosomally synthesized peptides via a cascade of post-translational modifications (PTMs).

Graphical abstract: Post-translational modifications involved in the biosynthesis of thiopeptide antibiotics
Communication

Is more better? A comparison of tri- and tetrapeptidic catalysts

From an enzymatic perspective, there is a general notion that the bigger and more complex a catalytically active peptide is the more enzyme-like and the better it should become. But is this really true?

Graphical abstract: Is more better? A comparison of tri- and tetrapeptidic catalysts
Communication

Novel chemoenzymatic oxidation of amines into oximes based on hydrolase-catalysed peracid formation

The efficient transformation of benzylamines into the corresponding oximes has been described by means of a chemoenzymatic process.

Graphical abstract: Novel chemoenzymatic oxidation of amines into oximes based on hydrolase-catalysed peracid formation
Open Access Paper

Structural insights into the ene-reductase synthesis of profens

New insight into the general utility of ene reductases for the synthesis of profens is established using structural and biocatalytic approaches.

Graphical abstract: Structural insights into the ene-reductase synthesis of profens
Open Access Paper

Exploring the reversal of enantioselectivity on a zinc-dependent alcohol dehydrogenase

Alcohol Dehydrogenase (ADH) enzymes catalyse the reversible reduction of prochiral ketones to the corresponding alcohols.

Graphical abstract: Exploring the reversal of enantioselectivity on a zinc-dependent alcohol dehydrogenase
Paper

A carbonyl reductase from Candida parapsilosis ATCC 7330: substrate selectivity and enantiospecificity

A purified carbonyl reductase (SRED) asymmetrically reduces ketones and α-ketoesters to (S)-alcohols with a potential application in the synthesis of industrially important chiral molecules.

Graphical abstract: A carbonyl reductase from Candida parapsilosis ATCC 7330: substrate selectivity and enantiospecificity
Paper

Improving target amino acid selectivity in a permissive aminoacyl tRNA synthetase through counter-selection

We report the selection and characterization of an improved acridon-2-ylalanine aminoacyl tRNA synthetase.

Graphical abstract: Improving target amino acid selectivity in a permissive aminoacyl tRNA synthetase through counter-selection
Paper

Multidrug resistance regulators (MDRs) as scaffolds for the design of artificial metalloenzymes

Artificial metalloenzymes were created from multidrug resistance regulator proteins by in vivo incorporation of an unnatural metal binding amino acid.

Graphical abstract: Multidrug resistance regulators (MDRs) as scaffolds for the design of artificial metalloenzymes
Paper

Inter- and intramolecular aldol reactions promiscuously catalyzed by a proline-based tautomerase

The enzyme 4-oxalocrotonate tautomerase, which has a catalytic amino-terminal proline, was shown to promiscuously catalyze inter- and intramolecular aldol reactions.

Graphical abstract: Inter- and intramolecular aldol reactions promiscuously catalyzed by a proline-based tautomerase
Paper

Converting Pasteurella multocida α2–3-sialyltransferase 1 (PmST1) to a regioselective α2–6-sialyltransferase by saturation mutagenesis and regioselective screening

A sialyltransferase mutant for selectively α2–6-sialylating terminal galactose in polyLacNAc-glycan was identified using a novel microtiter plate-based screening assay.

Graphical abstract: Converting Pasteurella multocida α2–3-sialyltransferase 1 (PmST1) to a regioselective α2–6-sialyltransferase by saturation mutagenesis and regioselective screening
Paper

Constrained evolution of a bispecific enzyme: lessons for biocatalyst design

Analysis of the natural evolution of bispecificity in triazine hydrolase highlights the importance of epistasis in protein engineering and evolution.

Graphical abstract: Constrained evolution of a bispecific enzyme: lessons for biocatalyst design
16 items

About this collection

Biocatalysis includes a range of topics related to catalysis by molecules obtained or derived from biology. This issue, Guest Edited by Professor Scott K. Silverman and Professor Douglas A. Mitchell, will cover the following topics:

1. Study of naturally occurring biological catalysts (protein enzymes and ribozymes)

2. Protein engineering and directed evolution, including computational tools

3. Metabolic engineering for synthetic chemistry

4. Development of synthetic biologically inspired catalysts, including small-molecule catalysts

Articles in this themed collection will be added below as soon as possible after they are published.

Please return to this page frequently to see the collection grow.

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