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Themed collection Enzymes and Proteins

46 articles
Feature Article

Electrostatic effects on (di)terpene synthase product outcome

Effect of specifically located hydroxyl group in counter-acting effect of pyrophosphate anion co-product in steering carbocation migration towards itself (e.g. by further cyclization, as shown).

Graphical abstract: Electrostatic effects on (di)terpene synthase product outcome
From the themed collection: Enzymes and Proteins
Feature Article

Cytochromes P450 as useful biocatalysts: addressing the limitations

Factors limiting the exploitation of cytochrome P450s and significant steps which have been taken towards addressing these limitations are discussed.

Graphical abstract: Cytochromes P450 as useful biocatalysts: addressing the limitations
From the themed collection: Enzymes and Proteins
Communication

Probing the stereoselectivity of P-glycoprotein—synthesis, biological activity and ligand docking studies of a set of enantiopure benzopyrano[3,4-b][1,4]oxazines

Enantiomerically pure benzopyrano[3,4-b][1,4]oxazines show significant differences in their inhibitory potency of P-glycoprotein. Docking studies provide first insights into potential binding sites for these compounds.

Graphical abstract: Probing the stereoselectivity of P-glycoprotein—synthesis, biological activity and ligand docking studies of a set of enantiopure benzopyrano[3,4-b][1,4]oxazines
From the themed collection: Enzymes and Proteins
Communication

A new dehydrogenase from Clostridium acetobutylicum for asymmetric synthesis: dynamic reductive kinetic resolution entry into the Taxotère side chain

Asymmetric carbonyl reduction using a new Clostridium acetobutylicum ADH provides access to diverse value-added chiral building blocks using biorenewable redox equivalents.

Graphical abstract: A new dehydrogenase from Clostridium acetobutylicum for asymmetric synthesis: dynamic reductive kinetic resolution entry into the Taxotère side chain
From the themed collection: Enzymes and Proteins
Communication

Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Analogues of Val and Leu having a chloro substituent in place of a methyl group are efficiently incorporated into protein.

Graphical abstract: Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis
From the themed collection: Enzymes and Proteins
Communication

Competing SN2 and E2 reaction pathways for hexachlorocyclohexane degradation in the gas phase, solution and enzymes

Quantum chemistry calculations reveal that the isomer specificity of enzymes that break down hexachlorocyclohexane is constrained by the intrinsic substrate reactivity.

Graphical abstract: Competing SN2 and E2 reaction pathways for hexachlorocyclohexane degradation in the gas phase, solution and enzymes
From the themed collection: Enzymes and Proteins
Communication

Ribonuclease S redux

Modern methods ease the use and enhance the versatility of an historical protein-fragment complementation system.

Graphical abstract: Ribonuclease S redux
From the themed collection: Enzymes and Proteins
Communication

Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes

Several single and double mutants from a minimal cytochrome P450 BM3 library were found to effectively oxidize cyclic and acyclic alkanes. For example, the F87A/A328V mutant hydroxylates cyclododecane to cyclododecanol.

Graphical abstract: Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes
From the themed collection: Enzymes and Proteins
Communication

Biocompatible functionalisation of starch

A combination of enzymatic synthesis and click chemistry is employed for the production of starch derivatives under mild, aqueous reaction conditions.

Graphical abstract: Biocompatible functionalisation of starch
From the themed collection: Enzymes and Proteins
Communication

A fast and sensitive assay for measuring the activity and enantioselectivity of transaminases

A fast and sensitive method for screening transaminase activity and enantioselectivity, using D- and L-amino acid oxidases, allows new amine substrates to be rapidly identified.

Graphical abstract: A fast and sensitive assay for measuring the activity and enantioselectivity of transaminases
From the themed collection: Enzymes and Proteins
Communication

Micelle to fibre biocatalytic supramolecular transformation of an aromatic peptide amphiphile

A mechanistic insight into a phosphatase-driven supramolecular transformation whereby a peptide amphiphile switches from micellar structure to a chiral nanofibre morphology.

Graphical abstract: Micelle to fibre biocatalytic supramolecular transformation of an aromatic peptide amphiphile
From the themed collection: Enzymes and Proteins
Communication

Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates

Tri-functional activity-based proteasome probes are used for a three-step chemical proteomics approach, in which proteasomal catalytic sites are covalently and irreversibly modified, followed by photocrosslinking of these to flanking subunits and Staudinger–Bertozzi ligation for visualization and identification of the resulting conjugates.

Graphical abstract: Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates
From the themed collection: Enzymes and Proteins
Communication

Photochemical cleavage of leader peptides

A photolabile linker is described for light-mediated removal of leader peptides involved in biosynthesis of posttranslationally modified peptide natural products.

Graphical abstract: Photochemical cleavage of leader peptides
From the themed collection: Enzymes and Proteins
Communication

Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

An aldehyde-containing substrate for protein farnesyltransferase was shown to be enzymatically incorporated into a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method provides an alternative to the commonly employed Cu(I)-catalyzed click reaction.

Graphical abstract: Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation
From the themed collection: Enzymes and Proteins
Communication

Towards preparative-scale, biocatalytic alkene reductions

Reaction strategies for employing two complementary alkene reductase enzymes in laboratory-scale conversions have been developed.

Graphical abstract: Towards preparative-scale, biocatalytic alkene reductions
From the themed collection: Enzymes and Proteins
Communication

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

Mutants of Bacillus subtilis lipase evolved for enhanced thermostability also show increased stability in organic solvents such as acetonitrile (ACN).

Graphical abstract: Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method
From the themed collection: Enzymes and Proteins
Communication

MhyADH catalysed Michael addition of water and in situ oxidation

MhyADH catalyses the Michael addition of water and also the subsequent oxidation to a diketone. In the absence of an oxidation reagent the reaction stops after the Michael addition.

Graphical abstract: MhyADH catalysed Michael addition of water and in situ oxidation
From the themed collection: Enzymes and Proteins
Communication

Tandem driven dynamic self-inhibition of acetylcholinesterase

A concept of tandem driven dynamic enzyme self-inhibition is demonstrated for acetylcholinesterase through the action of dynamic stealth inhibitors using reversible transthiolesterification.

Graphical abstract: Tandem driven dynamic self-inhibition of acetylcholinesterase
From the themed collection: Enzymes and Proteins
Communication

Profiling small molecule inhibitors against helix–receptor interactions: the Bcl-2 family inhibitor BH3I-1 potently inhibits p53/hDM2

We validate a practical methodology for the rapid profiling of small molecule inhibitors of protein–protein interactions. We find that a well known BH3 family inhibitor can potently inhibit the p53/hDM2 interaction.

Graphical abstract: Profiling small molecule inhibitors against helix–receptor interactions: the Bcl-2 family inhibitor BH3I-1 potently inhibits p53/hDM2
From the themed collection: Enzymes and Proteins
Communication

Cyclen-hybrid compound captures copper to protect INS-1 cells from islet amyloid polypeptide cytotoxicity by inhibiting and lysing effects

I1–Cu(II) inhibits hIAPP cytotoxicity by interfering with aggregation and cleavage.

Graphical abstract: Cyclen-hybrid compound captures copper to protect INS-1 cells from islet amyloid polypeptide cytotoxicity by inhibiting and lysing effects
From the themed collection: Enzymes and Proteins
Communication

Simultaneous iridium catalysed oxidation and enzymatic reduction employing orthogonal reagents

Employing orthogonal reaction conditions an iridium catalysed oxidative hydrogen transfer was successfully coupled concurrently to an asymmetric enzyme catalysed reduction.

Graphical abstract: Simultaneous iridium catalysed oxidation and enzymatic reduction employing orthogonal reagents
From the themed collection: Enzymes and Proteins
Communication

Structural and biochemical basis for the firm chemo- and regioselectivity of the nitro-forming N-oxygenase AurF

Variants of the N-oxygenase AurF from Streptomyces thioluteus selectively transform guanidyl- and amidinyl-substituted anilines into the corresponding nitro compounds. Site-directed mutagenesis and structural data provide new insights into regioselective N-oxygenation.

Graphical abstract: Structural and biochemical basis for the firm chemo- and regioselectivity of the nitro-forming N-oxygenase AurF
From the themed collection: Enzymes and Proteins
Communication

Fluorinase mediated chemoenzymatic synthesis of [18F]-fluoroacetate

A novel preparation of sodium [18F]-fluoroacetate is described by a fluorinase catalysed reaction of S-adenosyl-L-methionine 1 with no carrier added [18F]-fluoride and then the [18F]-2 product is oxidized to [18F]-fluoroacetate by a Kuhn–Roth oxidative degradation.

Graphical abstract: Fluorinase mediated chemoenzymatic synthesis of [18F]-fluoroacetate
From the themed collection: Enzymes and Proteins
Communication

Peptidomimetic inhibitors targeting the CCR5-binding site on the human immunodeficiency virus type-1 gp120 glycoprotein complexed to CD4

The display of two sulfotyrosine residues in the α-helical fold of CCR5 can be mimicked in a β-hairpin peptidomimetic.

Graphical abstract: Peptidomimetic inhibitors targeting the CCR5-binding site on the human immunodeficiency virus type-1 gp120 glycoprotein complexed to CD4
From the themed collection: Enzymes and Proteins
Communication

Ligand dimerization programmed by hybridization to study multimeric ligand–receptor interactions

Peptidic ligands (16mer macrocycles) were dimerized with different geometries programmed by hybridization to recapitulate the interaction of TRAIL.

Graphical abstract: Ligand dimerization programmed by hybridization to study multimeric ligand–receptor interactions
From the themed collection: Enzymes and Proteins
Communication

Structure–activity relationships of the phosphonate antibiotic dehydrophos

A series of analogs of dehydrophos were prepared and tested for their antimicrobial activity.

Graphical abstract: Structure–activity relationships of the phosphonate antibiotic dehydrophos
From the themed collection: Enzymes and Proteins
Communication

A fluopol-ABPP HTS assay to identify PAD inhibitors

A fluopol-ABPP HTS assay identifies streptonigrin as a potent and selective PAD4 inactivator.

Graphical abstract: A fluopol-ABPP HTS assay to identify PAD inhibitors
From the themed collection: Enzymes and Proteins
Communication

Interaction of PqqE and PqqD in the pyrroloquinoline quinone (PQQ) biosynthetic pathway links PqqD to the radical SAM superfamily

Data are presented that show PqqD interacting with PqqE.

Graphical abstract: Interaction of PqqE and PqqD in the pyrroloquinoline quinone (PQQ) biosynthetic pathway links PqqD to the radical SAM superfamily
From the themed collection: Enzymes and Proteins
Communication

Structural insights into substrate specificity and solvent tolerance in alcohol dehydrogenase ADH-‘A’ from Rhodococcus ruber DSM 44541

The first structures of the widely-used alcohol dehydrogenase ADH-‘A’ provide new avenues for the structure-guided engineering of this useful enzyme.

Graphical abstract: Structural insights into substrate specificity and solvent tolerance in alcohol dehydrogenase ADH-‘A’ from Rhodococcus ruber DSM 44541
From the themed collection: Enzymes and Proteins
Communication

Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration

The unfavourable equilibrium in a transamination with isopropylamine is displaced in an enzymatic cascade reaction using alcohol dehydrogenase in situ.

Graphical abstract: Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration
From the themed collection: Enzymes and Proteins
Communication

Chemoenzymatic asymmetric total synthesis of (S)-Rivastigmine using ω-transaminases

Asymmetric total synthesis of (S)-Rivastigmine was achieved via stereoselective biocatalytic transamination of acetophenone derivatives.

Graphical abstract: Chemoenzymatic asymmetric total synthesis of (S)-Rivastigmine using ω-transaminases
From the themed collection: Enzymes and Proteins
Communication

Rational creation of mutant enzyme showing remarkable enhancement of catalytic activity and enantioselectivity toward poor substrates

Catalytic activity and enantioselectivity of lipase toward poor substrates bearing bulky substituents on both sides have been dramatically improved by rational design; the E value was increased from 5 (wild-type enzyme) to >200 (I287F/I290A double mutant) with an acceleration of the reaction rate.

Graphical abstract: Rational creation of mutant enzyme showing remarkable enhancement of catalytic activity and enantioselectivity toward poor substrates
From the themed collection: Enzymes and Proteins
Communication

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

The first example of directed evolution of a novel P450pyr monooxygenase with inverted enantioselectivity has been demonstrated.

Graphical abstract: Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution
From the themed collection: Enzymes and Proteins
Communication

Silver catalyzed cascade synthesis of alkaloid ring systems: concise total synthesis of fascaplysin, homofascaplysin C and analogues

A silver catalyzed and microwave assisted one-pot cascade synthesis provides efficient access to diverse alkaloid-inspired scaffold classes, and a concise and efficient total synthesis of homofascaplysin C and fascaplysin.

Graphical abstract: Silver catalyzed cascade synthesis of alkaloid ring systems: concise total synthesis of fascaplysin, homofascaplysin C and analogues
From the themed collection: Enzymes and Proteins
Communication

Enzymatically triggered amyloid formation: an approach for studying peptide aggregation

A strategy has been demonstrated that utilizes a phosphatase as a natural tool for the triggering and control of amyloid formation in a coiled coil peptide model under conditions that closely approximate a physiological environment.

Graphical abstract: Enzymatically triggered amyloid formation: an approach for studying peptide aggregation
From the themed collection: Enzymes and Proteins
Communication

An unnatural amino acid that mimics phosphotyrosine

By replacing the phosphate group in pTyr with an isoxazole carboxylic acid, a novel unnatural amino acid has been successfully synthesized. Subsequently, its incorporation into a known PPI (protein–protein interaction) inhibitor of STAT3 protein (ISS 610) generated I2 which showed reasonable anti-STAT3 activity in both fluorescence polarization and cell-proliferation experiments.

Graphical abstract: An unnatural amino acid that mimics phosphotyrosine
From the themed collection: Enzymes and Proteins
Communication

Solid phase total synthesis of the 3-amino-6-hydroxy-2-piperidone (Ahp) cyclodepsipeptide and protease inhibitor Symplocamide A

The Ahp cyclodepsipeptide Symplocamide A was synthesized on solid phase, using an unsaturated amino acid as a key building block for the formation of the central Ahp moiety.

Graphical abstract: Solid phase total synthesis of the 3-amino-6-hydroxy-2-piperidone (Ahp) cyclodepsipeptide and protease inhibitor Symplocamide A
From the themed collection: Enzymes and Proteins
Communication

Computational design of orthogonal nucleoside kinases

Rosetta design software was employed to remodel the substrate specificity of Drosophila melanogaster 2′-deoxyribonucleoside kinase for efficient phosphorylation of the nucleoside analog prodrug 3′-deoxythymidine.

Graphical abstract: Computational design of orthogonal nucleoside kinases
From the themed collection: Enzymes and Proteins
Communication

O-Glycoligases, a new category of glycoside bond-forming mutant glycosidases, catalyse facile syntheses of isoprimeverosides

Near quantitative yields of alpha-xyloside products were obtained by coupling alpha-xylosyl fluoride with sugar acceptors using the acid/base mutant D482A of the GH31 alpha-xylosidase Yic-I

Graphical abstract: O-Glycoligases, a new category of glycoside bond-forming mutant glycosidases, catalyse facile syntheses of isoprimeverosides
From the themed collection: Enzymes and Proteins
Communication

Comparing dendritic with linear esterase peptides by screening SPOT arrays for catalysis

Direct fluorescence screening of a peptide library on a SPOT array identified oligohistidines as potent esterase peptides for the hydrolysis of acetoxypyrene trisulfonates.

Graphical abstract: Comparing dendritic with linear esterase peptides by screening SPOT arrays for catalysis
From the themed collection: Enzymes and Proteins
Communication

Characterization of xenobiotic reductase A (XenA): study of active site residues, substrate spectrum and stability

Characterization on xenobiotic reductase A and its mutants for enzyme functionality and stability.

Graphical abstract: Characterization of xenobiotic reductase A (XenA): study of active site residues, substrate spectrum and stability
From the themed collection: Enzymes and Proteins
Communication

Total chemical synthesis of human proinsulin

The protein enantiomers D- and L-proinsulin have been prepared by total synthesis using native chemical ligation.

Graphical abstract: Total chemical synthesis of human proinsulin
From the themed collection: Enzymes and Proteins
Communication

Total chemical synthesis and X-ray structure of kaliotoxin by racemic protein crystallography

The first X-ray structure of kaliotoxin, enabled by ‘one pot’ chemical ligation of synthetic peptides and racemic protein crystallography.

Graphical abstract: Total chemical synthesis and X-ray structure of kaliotoxin by racemic protein crystallography
From the themed collection: Enzymes and Proteins
Communication

Metabolic probes for imaging endosymbiotic bacteria within toxic dinoflagellates

We report a technique to visualize toxic dinoflagellates and suggest a preventative tool to warn of an impending danger and ensure the safe delivery of shellfish to the consumer market.

Graphical abstract: Metabolic probes for imaging endosymbiotic bacteria within toxic dinoflagellates
From the themed collection: Enzymes and Proteins
Communication

Phage display selection of miniprotein binders of the Estrogen Receptor

Phage display selection of disulfide stabilized miniproteins identified Estrogen Receptor binders displaying an LXXLL motif on a short peptide helix.

Graphical abstract: Phage display selection of miniprotein binders of the Estrogen Receptor
From the themed collection: Enzymes and Proteins
Communication

Engineering of an enantioselective tyrosine aminomutase by mutation of a single active site residue in phenylalanine aminomutase

An engineered tyrosine aminomutase with high enantioselectivity was constructed by homology-modelling based mutagenesis of the active site of phenylalanine aminomutase.

Graphical abstract: Engineering of an enantioselective tyrosine aminomutase by mutation of a single active site residue in phenylalanine aminomutase
From the themed collection: Enzymes and Proteins
46 articles

About this collection

This web-based themed issue showcases high quality papers in the field of chemical biology, specifically research that deals with enzymes and proteins. The collated, invited, and peer-reviewed ChemComm articles highlight cutting edge contributions by international leaders in the field.

The guest editors of this web themed issue are Professors Nicholas Turner, Wilfred van der Donk and Herbert Waldmann. They are delighted by the overwhelming response that the letter of invitation produced and they express their sincere thanks to the authors. The articles received and published cover a diverse range of contemporary topics in chemical biology including biosynthesis, biocatalysis, structure/function studies, drug design and proteins as sensors.

Articles in this web themed issue will be added to the list below as soon as possible, after they are published. Please return to this page frequently to see this collection grow.

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