Issue 30, 2010

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

Abstract

We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).

Graphical abstract: Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

Supplementary files

Article information

Article type
Communication
Submitted
02 Apr 2010
Accepted
13 May 2010
First published
03 Jun 2010

Chem. Commun., 2010,46, 5461-5463

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

W. L. Tang, Z. Li and H. Zhao, Chem. Commun., 2010, 46, 5461 DOI: 10.1039/C0CC00735H

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