Issue 45, 2010
From the journal:

Chemical Communications

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method†

Manfred T. Reetz,*a   Pankaj Soni,a   Layla Fernández,a   Yosephine Gumulyaa  and  José D. Carballeiraa  

* Corresponding authors

a Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany
E-mail: reetz@mpi-muelheim.mpg

Abstract

Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.

Graphical abstract: Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

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Article information

DOI
https://doi.org/10.1039/C0CC02657C
Article type
Communication
Submitted
19 Jul 2010
Accepted
01 Sep 2010
First published
18 Oct 2010

Chem. Commun., 2010,46, 8657-8658

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Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

M. T. Reetz, P. Soni, L. Fernández, Y. Gumulya and J. D. Carballeira, Chem. Commun., 2010, 46, 8657 DOI: 10.1039/C0CC02657C

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