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Themed collection In memory of Craig Lunte

51 items
Minireview

Recent advances in microscale western blotting

Recent innovations in microscale western blotting are surveyed, and the potential for enhancing detection using advances in label-free biosensing is briefly discussed.

Graphical abstract: Recent advances in microscale western blotting
From the themed collection: Recent Review Articles
Minireview

3D-printed microfluidic devices: fabrication, advantages and limitations—a mini review

A mini-review that focuses on the most recent trends in 3D-printed microfluidic devices, with sample designs that can be downloaded for use.

Graphical abstract: 3D-printed microfluidic devices: fabrication, advantages and limitations—a mini review
From the themed collection: Recent Review Articles
Communication

Towards low-cost bioanalytical tools for sarcosine assays for cancer diagnostics

Sarcosine is an amino acid that has been listed as a new indicator for prostate cancer.

Graphical abstract: Towards low-cost bioanalytical tools for sarcosine assays for cancer diagnostics
From the themed collection: In memory of Craig Lunte
Communication

A fully disposable paper-based electrophoresis microchip with integrated pencil-drawn electrodes for contactless conductivity detection

We describe the development of a paper electrophoresis chip integrated with pencil electrodes for contactless conductivity detection and its application in the separation of biomolecules associated with kidney dysfunctions.

Graphical abstract: A fully disposable paper-based electrophoresis microchip with integrated pencil-drawn electrodes for contactless conductivity detection
From the themed collection: In memory of Craig Lunte
Communication

Novel carbon-fiber microelectrode batch fabrication using a 3D-printed mold and polyimide resin

Utilization of polyimide resin and 3D printed mold provides a novel, rapid, and low cost alternative for carbon-fiber microelectrode fabrication.

Graphical abstract: Novel carbon-fiber microelectrode batch fabrication using a 3D-printed mold and polyimide resin
From the themed collection: In memory of Craig Lunte
Communication

Electrochromic sensing platform based on steric hindrance effects for CEA detection

In this work, an electrochromic sensing platform with prussian blue (PB) as the indicator was proposed for signaling carcinoembryonic antigen (CEA) using the bipolar electrode (BPE) system.

Graphical abstract: Electrochromic sensing platform based on steric hindrance effects for CEA detection
From the themed collection: In memory of Craig Lunte
Open Access Paper

Electrochemical behaviour at a liquid-organogel microinterface array of fucoidan extracted from algae

The electrochemical behaviour of fucoidan, a sulfated polysaccharide, was investigated, leading to a detection strategy by adsorptive stripping voltammetry.

Graphical abstract: Electrochemical behaviour at a liquid-organogel microinterface array of fucoidan extracted from algae
From the themed collection: In memory of Craig Lunte
Paper

New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration

Microfluidic discs integrating silica-C18 and carbon monolithic columns were developed for sample preparation.

Graphical abstract: New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration
From the themed collection: In memory of Craig Lunte
Paper

Detection of reactive aldehyde biomarkers in biological samples using solid-phase extraction pre-concentration and liquid chromatography with fluorescence detection

Fluorescence derivatization allows for detection of lipid peroxidation biomarkers of epilepsy-induced oxidative stress.

Graphical abstract: Detection of reactive aldehyde biomarkers in biological samples using solid-phase extraction pre-concentration and liquid chromatography with fluorescence detection
From the themed collection: In memory of Craig Lunte
Paper

Determination of phenolic acids in a range of Irish whiskies, including single pot stills and aged single malts, using capillary electrophoresis with field amplified sample stacking

Phenolic acids in Irish whiskeys were determined using CE-UV with field amplified sample stacking. Length of aging and prior use of cask influenced phenolic acid profiles.

Graphical abstract: Determination of phenolic acids in a range of Irish whiskies, including single pot stills and aged single malts, using capillary electrophoresis with field amplified sample stacking
From the themed collection: In memory of Craig Lunte
Paper

Monitoring carnosine uptake by RAW 264.7 macrophage cells using microchip electrophoresis with fluorescence detection

A microfluidic platform was used to investigate the antioxidant properties of carnosine, an endogenous dipeptide.

Graphical abstract: Monitoring carnosine uptake by RAW 264.7 macrophage cells using microchip electrophoresis with fluorescence detection
From the themed collection: In memory of Craig Lunte
Paper

Trapping of Au nanoparticles in a microfluidic device using dielectrophoresis for surface enhanced Raman spectroscopy

In this paper, we use DEP with insulating structures (iDEP) to generate a non-uniform electric field for trapping gold nanoparticles (AuNP). The system was coupled to a Raman spectrometer for the detection of Crystal Violet by utilizing the SERS effect.

Graphical abstract: Trapping of Au nanoparticles in a microfluidic device using dielectrophoresis for surface enhanced Raman spectroscopy
From the themed collection: In memory of Craig Lunte
Paper

Online fluorescence anisotropy immunoassay for monitoring insulin secretion from islets of Langerhans

Insulin secretion from islets of Langerhans is a dynamic process that is essential for maintaining glucose homeostasis.

Graphical abstract: Online fluorescence anisotropy immunoassay for monitoring insulin secretion from islets of Langerhans
From the themed collection: In memory of Craig Lunte
Paper

Checkpoints for preliminary identification of small molecules found enriched in autophagosomes and activated mast cell secretions analyzed by comparative UPLC/MSe

Checkpoints in processing of metabolomics data are essential to reliably define preliminary identifications of metabolomic studies.

Graphical abstract: Checkpoints for preliminary identification of small molecules found enriched in autophagosomes and activated mast cell secretions analyzed by comparative UPLC/MSe
From the themed collection: Recent HOT articles
Paper

A kanamycin sensor based on an electrosynthesized molecularly imprinted poly-o-phenylenediamine film on a single-walled carbon nanohorn modified glassy carbon electrode

The application of a single-walled carbon nanohorn for a molecularly imprinted sensor has been demonstrated for the first time.

Graphical abstract: A kanamycin sensor based on an electrosynthesized molecularly imprinted poly-o-phenylenediamine film on a single-walled carbon nanohorn modified glassy carbon electrode
From the themed collection: In memory of Craig Lunte
Paper

Degassed PDMS pump for controlled extraction from dried filter samples in microfluidic devices

Simplified sample collection methods can continue to advance the field of biosensors by decreasing the cost required to acquire and store samples and/or increasing the number of locations where the sensors can be used.

Graphical abstract: Degassed PDMS pump for controlled extraction from dried filter samples in microfluidic devices
From the themed collection: In memory of Craig Lunte
Paper

Characterization of activated cyclic olefin copolymer: effects of ethylene/norbornene content on the physiochemical properties

The ethylene/norbornene content within cyclic olefin copolymer (COC) is well known to affect the chemical and physical properties of the copolymer, such as the glass transition temperature (Tg) and transparency.

Graphical abstract: Characterization of activated cyclic olefin copolymer: effects of ethylene/norbornene content on the physiochemical properties
From the themed collection: In memory of Craig Lunte
Paper

Fast voltammetry of metals at carbon-fiber microelectrodes: towards an online speciation sensor

Speciation controls the chemical behavior of trace metals.

Graphical abstract: Fast voltammetry of metals at carbon-fiber microelectrodes: towards an online speciation sensor
From the themed collection: In memory of Craig Lunte
Paper

Fibre-based electrofluidics on low cost versatile 3D printed platforms for solute delivery, separations and diagnostics; from small molecules to intact cells

A novel fibre-based microfluidic methodology was developed to move and isolate charged solutes, biomolecules, and intact bacterial cells, based upon a novel multi-functional 3D printed platform.

Graphical abstract: Fibre-based electrofluidics on low cost versatile 3D printed platforms for solute delivery, separations and diagnostics; from small molecules to intact cells
From the themed collection: In memory of Craig Lunte
Paper

Detection of evoked acetylcholine release in mouse brain slices

The study of transmitter interactions in the brain requires methodology to detect stimulus-driven neurotransmitter release. This report introduces an enzyme-coated 7 μm carbon-fiber microelectrode used with fast-scan cyclic voltammetry to detect evoked acetylcholine release in mouse brain slices.

Graphical abstract: Detection of evoked acetylcholine release in mouse brain slices
From the themed collection: Recent HOT articles
Paper

Determination of L-DOPA at an optimized poly(caffeic acid) modified glassy carbon electrode

A glassy carbon electrode was modified by electrodeposition of a thin layer of poly(caffeic acid) and optimized for highly selective and sensitive detection of L-DOPA.

Graphical abstract: Determination of l-DOPA at an optimized poly(caffeic acid) modified glassy carbon electrode
From the themed collection: In memory of Craig Lunte
Paper

On-chip fluorescent labeling using reversed-phase monoliths and microchip electrophoretic separations of selected preterm birth biomarkers

On-chip preconcentration, purification, and fluorescent labeling are desirable sample preparation steps to achieve complete automation in integrated microfluidic systems.

Graphical abstract: On-chip fluorescent labeling using reversed-phase monoliths and microchip electrophoretic separations of selected preterm birth biomarkers
From the themed collection: In memory of Craig Lunte
Paper

Absorption spectroscopy for the quantitative prediction of lanthanide concentrations in the 3LiCl–2CsCl eutectic at 723 K

The absorption spectra of single-component mixtures of six lanthanide chloride salts were obtained in the molten salt eutectic 3LiCl–2CsCl at 723 K, and used to build multivariate regression models to predict concentrations of multi-component mixtures of these metals in solution.

Graphical abstract: Absorption spectroscopy for the quantitative prediction of lanthanide concentrations in the 3LiCl–2CsCl eutectic at 723 K
From the themed collection: In memory of Craig Lunte
Paper

Whole blood analysis using microfluidic plasma separation and enzyme-linked immunosorbent assay devices

In this study, a microfluidic plasma-separation device that realizes the whole blood analysis of C-reactive protein (CRP) using one drop of blood is developed.

Graphical abstract: Whole blood analysis using microfluidic plasma separation and enzyme-linked immunosorbent assay devices
From the themed collection: In memory of Craig Lunte
Paper

Colorimetric analysis of the decomposition of S-nitrosothiols on paper-based microfluidic devices

A disposable paper microfluidic device was developed to analyse different S-nitrosothiols simultaneously decomposed by Hg2+ as well as UV, Vis and IR lamps.

Graphical abstract: Colorimetric analysis of the decomposition of S-nitrosothiols on paper-based microfluidic devices
From the themed collection: In memory of Craig Lunte
Open Access Paper

Real-time detection of carboplatin using a microfluidic system

A novel on-line microfluidic assay for the chemotherapy agent carboplatin will allow sensitive detection of the drug directly applied to the tumour as it emerges from the tumour into healthy tissue.

Graphical abstract: Real-time detection of carboplatin using a microfluidic system
From the themed collection: Recent Open Access Articles
Paper

Microchip in situ electrosynthesis of silver metallic oxide clusters for ultra-FAST detection of galactose in galactosemic newborns’ urine samples

This work describes for the first time the coupling of microfluidic chips (MC) to electrosynthetized silver metallic oxide clusters (AgMOCs).

Graphical abstract: Microchip in situ electrosynthesis of silver metallic oxide clusters for ultra-FAST detection of galactose in galactosemic newborns’ urine samples
From the themed collection: Recent HOT articles
Paper

Evaluation of a nitrogen-incorporated tetrahedral amorphous carbon thin film for the detection of tryptophan and tyrosine using flow injection analysis with amperometric detection

We report on the analytical performance of a tetrahedral amorphous carbon (ta-C:N) thin-film electrode in flow injection analysis with amperometric detection.

Graphical abstract: Evaluation of a nitrogen-incorporated tetrahedral amorphous carbon thin film for the detection of tryptophan and tyrosine using flow injection analysis with amperometric detection
From the themed collection: In memory of Craig Lunte
Paper

Closed bipolar electrode-enabled dual-cell electrochromic detectors for chemical sensing

Battery operation of a closed-BPE dual cell with colorimetric readout by smartphone camera yields a simple, inexpensive, field-deployable electrochemical sensor.

Graphical abstract: Closed bipolar electrode-enabled dual-cell electrochromic detectors for chemical sensing
From the themed collection: In memory of Craig Lunte
Paper

Development of a protease-resistant reporter to quantify BCR–ABL activity in intact cells

A peptidase-resistant ABL kinase substrate was developed by identifying protease-susceptible bonds on an ABL substrate peptide and replacing flanking amino acids with non-native amino acids.

Graphical abstract: Development of a protease-resistant reporter to quantify BCR–ABL activity in intact cells
From the themed collection: In memory of Craig Lunte
Paper

Location-specific immunodetection of cocaine on banknotes

A novel in-gel bioanalytical immunodetection method has been developed to determine both the presence and the location of cocaine on the surface of banknotes.

Graphical abstract: Location-specific immunodetection of cocaine on banknotes
From the themed collection: In memory of Craig Lunte
Paper

Fast voltammetry of metals at carbon-fiber microelectrodes: rapid determination of solution formation constants

FSCV can be utilized to predict solution formation constants.

Graphical abstract: Fast voltammetry of metals at carbon-fiber microelectrodes: rapid determination of solution formation constants
From the themed collection: In memory of Craig Lunte
Paper

A rotationally-driven polyethylene terephthalate microdevice with integrated reagent mixing for multiplexed PCR amplification of DNA

We demonstrate the capabilities of a centrifugal polyethylene terephthalate toner (PeT) microdevice for genetic analysis of short tandem repeats (STR) via PCR amplification.

Graphical abstract: A rotationally-driven polyethylene terephthalate microdevice with integrated reagent mixing for multiplexed PCR amplification of DNA
From the themed collection: In memory of Craig Lunte
Paper

Inkjet printing on transparency films for reagent storage with polyester–toner microdevices

The use of overhead transparencies as substrates for the fabrication of microfluidic devices brings new capabilities in the creation of fully-integrated systems.

Graphical abstract: Inkjet printing on transparency films for reagent storage with polyester–toner microdevices
From the themed collection: In memory of Craig Lunte
Paper

Detection of mSiglec-E, in solution and expressed on the surface of Chinese hamster ovary cells, using sialic acid functionalised gold nanoparticles

Glyconanoparticles have been synthesised for the specific detection of mSiglec-E in solution and expressed on the surface of CHO cells.

Graphical abstract: Detection of mSiglec-E, in solution and expressed on the surface of Chinese hamster ovary cells, using sialic acid functionalised gold nanoparticles
From the themed collection: In memory of Craig Lunte
Paper

Microfluidic array for simultaneous detection of DNA oxidation and DNA-adduct damage

A microfluidic electrochemical sensor array detects DNA oxidation and adduct formation for a fast genotoxicity chemistry screen for test compounds.

Graphical abstract: Microfluidic array for simultaneous detection of DNA oxidation and DNA-adduct damage
From the themed collection: Recent HOT articles
Paper

A quantitative, in vitro appraisal of experimental low-glucose storage solutions used for blood banking

A combination of 3D printed tools and classic cellular analyses reveals the benefits of reducing the glucose content of currently approved solutions used to process and store red blood cells for transfusion medicine.

Graphical abstract: A quantitative, in vitro appraisal of experimental low-glucose storage solutions used for blood banking
From the themed collection: In memory of Craig Lunte
Open Access Paper

Imaging of localized enzymatic peroxidase activity over unbiased individual gold nanowires by scanning electrochemical microscopy

Scanning electrochemical microscopy (SECM) in the constant-height mode was used to image individual unbiased gold nanowires (AuNWs, 2–3 μm long and ~140 nm diameter) covalently linked with horseradish peroxidase (HRP).

Graphical abstract: Imaging of localized enzymatic peroxidase activity over unbiased individual gold nanowires by scanning electrochemical microscopy
From the themed collection: In memory of Craig Lunte
Paper

Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis

Dengue diagnosis was performed on printed microzones based on colorimetric detection of IgG and IgM biomarkers in serum samples through enzyme-linked-immunosorbent assay experiments.

Graphical abstract: Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis
From the themed collection: In memory of Craig Lunte
Paper

Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

An air focusing technique was used to directly electrospin fibers into fully sealed microfluidic devices for 3D cell culture.

Graphical abstract: Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices
From the themed collection: In memory of Craig Lunte
Paper

Disulfide bond characterization of endogenous IgG3 monoclonal antibodies using LC-MS: an investigation of IgG3 disulfide-mediated isoforms

The use of monoclonal antibodies (mAbs) for the manufacture of innovator and biosimilar biotherapeutics has increased tremendously in recent years.

Graphical abstract: Disulfide bond characterization of endogenous IgG3 monoclonal antibodies using LC-MS: an investigation of IgG3 disulfide-mediated isoforms
From the themed collection: In memory of Craig Lunte
Paper

Expanding neurochemical investigations with multi-modal recording: simultaneous fast-scan cyclic voltammetry, iontophoresis, and patch clamp measurements

Simultaneous patch clamp and fast-scan cyclic voltammetry measurements were performed to monitor cell behavior during iontophoretic drug delivery.

Graphical abstract: Expanding neurochemical investigations with multi-modal recording: simultaneous fast-scan cyclic voltammetry, iontophoresis, and patch clamp measurements
From the themed collection: Recent HOT articles
Paper

Detecting levels of polyquaternium-10 (PQ-10) via potentiometric titration with dextran sulphate and monitoring the equivalence point with a polymeric membrane-based polyion sensor

This manuscript reports a novel and facile method for PQ-10/SLS separation and subsequent PQ-10 quantification via potentiometric titration.

Graphical abstract: Detecting levels of polyquaternium-10 (PQ-10) via potentiometric titration with dextran sulphate and monitoring the equivalence point with a polymeric membrane-based polyion sensor
From the themed collection: In memory of Craig Lunte
Paper

Development of a novel method for the bioanalysis of benfotiamine and sulbutiamine in cancer cells

Quantification of benfotiamine and sulbutiamine, synthetic thiamine analogs, in biological samples is an essential step toward understanding the role of these thiamine analogs on cancer cell proliferation.

Graphical abstract: Development of a novel method for the bioanalysis of benfotiamine and sulbutiamine in cancer cells
From the themed collection: In memory of Craig Lunte
Paper

A novel ratiometric fluorescent immunoassay for human α-fetoprotein based on carbon nanodot-doped silica nanoparticles and FITC

Non-toxic, fluorescent carbon nanodot labels are employed as novel ratiometric immunosensors for α-fetoprotein (AFP), a liver cancer biomarker. The assay generates a broad linear range, a low detection limit, and can be adapted to a variety of immunoassay targets.

Graphical abstract: A novel ratiometric fluorescent immunoassay for human α-fetoprotein based on carbon nanodot-doped silica nanoparticles and FITC
From the themed collection: In memory of Craig Lunte
Paper

A label-free Sirtuin 1 assay based on droplet-electrospray ionization mass spectrometry

A reliable, label-free, ion suppression-free Sirtuin 1 assay has been developed. By interfacing multi-well plates to electrospray ionization mass spectrometry by oil-segmented droplets, the assay can be applied for high throughput Sirtuin 1 modulator screening.

Graphical abstract: A label-free Sirtuin 1 assay based on droplet-electrospray ionization mass spectrometry
From the themed collection: Recent HOT articles
Paper

A capillary electrophoresis electrospray ionization-mass spectrometry method using a borate background electrolyte for the fingerprinting analysis of flavonoids in Ginkgo biloba herbal supplements

A sheath liquid capillary electrophoresis-mass spectrometry interface was used to develop a qualitative method for analysis of 14 flavones, flavonols, flavonones, and representative glycosides in plant samples.

Graphical abstract: A capillary electrophoresis electrospray ionization-mass spectrometry method using a borate background electrolyte for the fingerprinting analysis of flavonoids in Ginkgo biloba herbal supplements
From the themed collection: In memory of Craig Lunte
Paper

A microporous silk carbon–ionic liquid composite for the electrochemical sensing of dopamine

A metal-free silk carbon–ionic liquid composite, synthesized from natural silk cocoons, was prepared for electrochemical determination of dopamine.

Graphical abstract: A microporous silk carbon–ionic liquid composite for the electrochemical sensing of dopamine
From the themed collection: In memory of Craig Lunte
Paper

Measurement of total cholesterol using an enzyme sensor based on a printed hydrogen peroxide electrocatalyst

An electrochemical sensor for measuring total cholesterol in serum using Triton X-100 as a printed, non-enzymatic electrocatalyst for hydrogen peroxide and for the solubilisation of lipoproteins.

Graphical abstract: Measurement of total cholesterol using an enzyme sensor based on a printed hydrogen peroxide electrocatalyst
From the themed collection: Recent HOT articles
Paper

Online electrochemical system as an in vivo method to study dynamic changes of ascorbate in rat brain during 3-methylindole-induced olfactory dysfunction

This study demonstrates the application of an OECS as an in vivo method to investigate the dynamic change of ascorbate in the olfactory bulb of rats during the acute period of olfactory dysfunction.

Graphical abstract: Online electrochemical system as an in vivo method to study dynamic changes of ascorbate in rat brain during 3-methylindole-induced olfactory dysfunction
From the themed collection: In memory of Craig Lunte
Paper

Segmented flow sampling with push–pull theta pipettes

We report development of a mobile and easy-to-fabricate theta pipette microfluidic device for segmented flow sampling.

Graphical abstract: Segmented flow sampling with push–pull theta pipettes
From the themed collection: In memory of Craig Lunte
51 items

About this collection

Professor Craig E. Lunte, who sadly passed away on April 13, 2015 at the age of 57, was a leader in the field of bioanalytical chemistry and a pioneer in the use of microdialysis sampling for drug metabolism studies. During his career, he developed many novel separation and detection methods for the analysis of microdialysis and other biological samples. He also developed several novel electrochemical detectors for liquid chromatography and electrophoresis and was an active member of the separations community as well. Whether in front of a classroom full of eager minds or in the laboratory providing hands-on instruction to curious students, Craig never missed an opportunity to share his wealth of knowledge.

In this tribute to Craig, we have put together a collection of papers invited from his peers, colleagues, and friends and have published it in Analyst and Analytical Methods. Together, these papers cover the fields of bioanalytical chemistry, microseparations, sampling, electrochemistry, pharmacokinetics, and mass spectrometry. We hope that this collection will be a fitting memorial to an inspiring teacher and remarkable chemist.

November 2015.

All papers in the collection are free to access using the links below. New articles will be added to this collection as they are published.

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