Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 42, 2016
Previous Article Next Article

Whole blood analysis using microfluidic plasma separation and enzyme-linked immunosorbent assay devices

Author affiliations

Abstract

In this study, a microfluidic plasma-separation device that realizes the whole blood analysis of C-reactive protein (CRP) using one drop of blood is developed. A small, fast, and easy-to-operate blood-testing device is desirable for point-of-care testing, home medical care, and medical cost reduction. Recently, significant advances have been made in analytical instrumentation, and an enzyme-linked immunosorbent assay (ELISA) of a 1 μL plasma sample has previously been realized. Moreover, recent developments in microfluidics have led to the miniaturization of analytical instruments. However, analytical centrifuges that are conventionally used for the separation of plasma and blood cells are very large and cumbersome to combine with microfluidic devices. Therefore, we propose a plasma-separation device combining microfluidics and membrane filtering. Herein, the proposed plasma-separation device is applied to whole blood analysis using one drop of blood. Specifically, the device is designed and fabricated to realize high plasma-separation efficiency via the evaluation of fluidic resistance of a porous membrane. Then, the quality of the plasma separated using the device is evaluated and compared with that of the plasma separated using a conventional centrifuge. Our results reveal that the microfluidic plasma-separation device can efficiently provide a sample for the ELISA of CRP using one drop (50 μL) of whole blood. The total analysis time including plasma separation and ELISA is approximately 25 min.

Graphical abstract: Whole blood analysis using microfluidic plasma separation and enzyme-linked immunosorbent assay devices

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 22 Jun 2016, accepted on 30 Sep 2016 and first published on 03 Oct 2016


Article type: Paper
DOI: 10.1039/C6AY01779G
Citation: Anal. Methods, 2016,8, 7597-7602
  •   Request permissions

    Whole blood analysis using microfluidic plasma separation and enzyme-linked immunosorbent assay devices

    H. Shimizu, M. Kumagai, E. Mori, K. Mawatari and T. Kitamori, Anal. Methods, 2016, 8, 7597
    DOI: 10.1039/C6AY01779G

Search articles by author