Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 22, 2016
Previous Article Next Article

Colorimetric analysis of the decomposition of S-nitrosothiols on paper-based microfluidic devices

Author affiliations


A disposable microfluidic paper-based analytical device (μPAD) was developed to easily analyse different S-nitrosothiols (RSNOs) through colorimetric measurements. RSNOs are carriers of nitric oxide (NO) that play several physiological and physiopathological roles. The quantification of RSNOs relies on their decomposition using several protocols and the colorimetric detection of the final product, NO or nitrite. μPADs were fabricated by wax printing technology in a geometry containing one central zone for the sample inlet and eight circular detection zones interconnected by microfluidic channels for decomposition and posterior detection of decayed products. Different decomposition protocols including mercuric ions and light (UV, visible, and infrared) were tested on μPADs. For this purpose, a 3D printed holder was coupled with μPADs to easily design a simultaneous decomposition procedure using different light sources. The Griess reagent was added to detect NO and nitrite produced by the different decomposition methods. μPADs were then scanned using a flat board scanner and calibration curves based on color intensity were plotted. The limit of detection (LOD) values achieved for nitrite (used as a reference compound) and S-nitrosoglutathione (GSNO) using mercuric decomposition were 3 and 4 μM, respectively. The LOD reported herein for nitrite is considered among the lowest LODs already reported for this compound using μPADs. The results also show that low-molecular-weight RSNO, namely S-nitrosocysteine, decomposes more easily than high-molecular-weight RSNOs with light. As a proof of concept, RSNOs in human plasma were successfully detected on μPADs. For this purpose, a preliminary treatment step was optimized and the presence of high-molecular-weight (HMW) RSNOs was evidenced in the available plasma samples. The concentrations of HMW-RSNOs and nitrite in the various samples ranged from 5 to 16 μM and from 37 to 58 μM, respectively.

Graphical abstract: Colorimetric analysis of the decomposition of S-nitrosothiols on paper-based microfluidic devices

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 24 Jun 2016, accepted on 07 Sep 2016 and first published on 08 Sep 2016

Article type: Paper
DOI: 10.1039/C6AN01439A
Citation: Analyst, 2016,141, 6314-6320
  •   Request permissions

    Colorimetric analysis of the decomposition of S-nitrosothiols on paper-based microfluidic devices

    A. Ismail, M. O. Araújo, C. L. S. Chagas, S. Griveau, F. D'Orlyé, A. Varenne, F. Bedioui and W. K. T. Coltro, Analyst, 2016, 141, 6314
    DOI: 10.1039/C6AN01439A

Search articles by author