Themed collection Biocatalysis & Bioprocessing
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Route selection and reaction engineering for sustainable metabolite synthesis
Great advances in tools and methodologies along the whole workflow are providing sustainable routes to a desired metabolite which can replace extractive manufacturing from endangered biological species or lengthy chemical routes from fossil-based starting materials.
React. Chem. Eng., 2023,8, 2109-2118
https://doi.org/10.1039/D3RE00222E
Continuous flow-mode synthesis of (chiral) amines with transaminase: a strategic biocatalytic approach to essential building blocks
Amines, and in particular chiral amines are essential building blocks for the manufacture of active pharmaceutical ingredients. Continuous flow syntheses using immobilized transaminases pave the way to green and intensified production processes.
React. Chem. Eng., 2023,8, 1505-1544
https://doi.org/10.1039/D3RE00210A
Enzyme immobilization on a 3D-printed reactor for aldehyde oxidation to carboxylic acid under mild conditions
An aldehyde dehydrogenase, catalyzing the oxidation of aldehydes under mild conditions, was immobilized on a 3D-printed reactor.
React. Chem. Eng., 2023,8, 543-547
https://doi.org/10.1039/D2RE00547F
Biocatalytic fumarate synthesis from pyruvate and CO2 as a feedstock
The biocatalytic synthesis of fumarate from CO2 and pyruvate viaL-malate as an intermediate in an aqueous medium using a biocatalytic system consisting of malate dehydrogenase and fumarase in the presence of NADH is developed.
React. Chem. Eng., 2022,7, 1931-1935
https://doi.org/10.1039/D2RE00039C
Automated high throughput workflow for rapid implementation of immobilized enzymes in chemical process development
Automated high throughput workflow facilitating the rapid optimization and development of immobilized enzymes – ready to be scaled – and thus matching the need for speed in modern drug development.
React. Chem. Eng., 2024,9, 1325-1333
https://doi.org/10.1039/D3RE00704A
Enzyme-modified amphiphilic polymer nanoparticles as high-performance Pickering interface biocatalysts
Enzyme-decorated polymeric nanoparticles as both catalytic sites and stabilizers for a high catalytic activity and easily recyclable Pickering interface biocatalysis system.
React. Chem. Eng., 2023,8, 3162-3170
https://doi.org/10.1039/D3RE00381G
Automated instant labeling chemistry workflow for real-time monitoring of monoclonal antibody N-glycosylation
Integration of Instant Procainamide (Instant-PC) flow chemistry into the N-GLYcanyzer PAT sequential injection system enables automated real-time monitoring of mAb N-glycosylation dynamics to facilitate advanced biologics manufacturing processes.
React. Chem. Eng., 2023,8, 2423-2434
https://doi.org/10.1039/D2RE00568A
Magnetic separation of immobilized biocatalyst enables continuous manufacturing with a solids-forming reaction
A new application of magnetic separation suitable for micron-scale solid–solid separation is designed and demonstrated on a pilot plant scale synthesis of amoxicillin trihydrate.
React. Chem. Eng., 2023,8, 2323-2331
https://doi.org/10.1039/D3RE00214D
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Aromatic hydroxylation of substituted benzenes by an unspecific peroxygenase from Aspergillus brasiliensis
An unspecific peroxygenase from Aspergillus brasiliensis highly expressed in Pichia pastoris catalyses aromatic hydroxylation of a number of benzylic compounds.
React. Chem. Eng., 2023,8, 2177-2186
https://doi.org/10.1039/D3RE00209H
You get what you screen for: a benchmark analysis of leaf branch compost cutinase variants for polyethylene terephthalate (PET) degradation
Reference enzymes allow PET hydrolases and protein engineering approaches to be compared across different substrates, their properties and reaction conditions. Using this concept, semi-rational protein design led to an improved PET hydrolase variant.
React. Chem. Eng., 2023,8, 2156-2169
https://doi.org/10.1039/D3RE00056G
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Directed evolution of Rhodotorula gracilisD-amino acid oxidase using single-cell hydrogel encapsulation and ultrahigh-throughput screening
A high-throughput screening platform uses peroxidase-mediated proximity labeling with fluorescent hydrogels. A yeast displayed enzyme variant library was screened using a reaction cascade. Enhancement of enzyme Km is observed for displayed enzymes.
React. Chem. Eng., 2023,8, 1960-1968
https://doi.org/10.1039/D3RE00002H
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Soybean peroxidase immobilised on cellulose-alginate hydrogels for removal of recalcitrant organic pollutants in water
Biodegradable, waste-derived hydrogels containing immobilised peroxidase were successfully used for enzymatic removal of organic pollutants in water. Hydrogels are reusable and provide detoxification of the contaminants solution.
React. Chem. Eng., 2023,8, 1629-1637
https://doi.org/10.1039/D3RE00009E
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Crystallization-based downstream processing of ω-transaminase- and amine dehydrogenase-catalyzed reactions
This study highlights the use of selective crystallization as a downstream-processing concept for amine products from biocatalytic reactions.
React. Chem. Eng., 2023,8, 1427-1439
https://doi.org/10.1039/D2RE00496H
One-pot cascade reactions for the synthesis of dinitroalkanes in aqueous buffer
A one-pot combination of biocatalysis and organocatalysis converts aliphatic alcohols to dinitroalkanes in mild conditions at room temperature.
React. Chem. Eng., 2023,8, 1152-1158
https://doi.org/10.1039/D2RE00403H
A robust and efficient lipase based nanobiocatalyst for phenothiazinyl-ethanol resolution
A new robust, active and selective biocatalyst, prepared by CaL-B entrapment into polyvinyl alcohol–chitosan nanofibers, proved to be efficient for the EKR of nine racemic phenothiazinyl-ethanols, important chiral building blocks.
React. Chem. Eng., 2023,8, 1109-1116
https://doi.org/10.1039/D2RE00515H
Process optimization using machine learning enhanced design of experiments (DOE): ranibizumab refolding as a case study
An application of ML enhanced DOE has been demonstrated to capture the process nonlinearity more effectively. The proposed approach is applied for modelling and optimization of protein refolding, resulting in 3% improved prediction efficiency.
React. Chem. Eng., 2023,8, 592-603
https://doi.org/10.1039/D2RE00440B
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Data-rich process development of immobilized biocatalysts in flow
The authors describe an automated, data-rich screening and process development method for rapid discovery, development, and optimization of immobilized enzymes, critical to many biocatalytic processes.
React. Chem. Eng., 2022,7, 866-876
https://doi.org/10.1039/D1RE00298H
About this collection
This special collection of Reaction Chemistry & Engineering, Guest Edited by Francesca Paradisi (University of Bern, Switzerland) Andreas Bommarius (Georgia Institute of Technology, USA) and Kai Guo (Nanjing Tech University, China) showcases cutting-edge advances in the areas of biocatalysis and bioprocessing. The research works featured in this collection cover the broad spectrum of bioprocessing research; highlighting work improving the efficiency of reactions, utilising cells and their components, and influencing the future directions of this field.