Issue 46, 2021

Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 are producers of the antibiotic nucleocidin and 4′-fluoroadenosine is identified as a co-product

Abstract

Genome homology and the presence of a putative biosynthetic gene cluster identified Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 as candidate producers of the antibiotic nucleocidin 1. Indeed when these bacterial strains were cultured in a medium supplemented with fluoride (4 mM) they each produced nucleocidin 1 and the previously identified 4′-fluoro-3′-O-β-glucosylated adenosine 2 and its sulfamylated derivative 3. In both of these cases 4′-fluoroadenosine 9 is also identified as a natural product although it has never been observed during fermentations of Streptomyces calvus, the original source of nucleocidin 1. The identity of 4′-fluoroadenosine 9 was confirmed by a total synthesis as well as by its in vitro enzymatic conversion to metabolite 2 using the glucosyl transferase enzyme, NucGT.

Graphical abstract: Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 are producers of the antibiotic nucleocidin and 4′-fluoroadenosine is identified as a co-product

Supplementary files

Article information

Article type
Communication
Submitted
24 Sept. 2021
Accepted
11 Okt. 2021
First published
03 Nov. 2021

Org. Biomol. Chem., 2021,19, 10081-10084

Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 are producers of the antibiotic nucleocidin and 4′-fluoroadenosine is identified as a co-product

Y. Chen, Q. Zhang, X. Feng, M. Wojnowska and D. O'Hagan, Org. Biomol. Chem., 2021, 19, 10081 DOI: 10.1039/D1OB01898A

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