The gelation process and protein absorption property of injectable SA-CMBC hydrogel used for procoagulant material

Abstract

Carboxymethylated bacterial cellulose (CMBC) was composited with sodium alginate (SA) to obtain SA-CMBC hydrogel, cross-linked by calcium ion, which was generated from the hydrolyzing of calcium carbonate with the addition of glucono-delta-lactone (GDL). The addition of CMBC could regulate the properties of SA-CMBC hydrogel, such as gelation time, mechanical property, protein absorption and procoagulant property. The addition of CMBC shortened the gelation time, the shortest of which reached to 4.9 min, and increased the mechanical property, the best of which reached to 0.118 MPa. The hydrogel with 25% weight fraction of CMBC showed the best protein absorption property. The procoagulant activity of the SA-CMBC hydrogel was investigated by activated partial thromboplastin time (APTT) and prothrombin time (PT), and hydrogel with 20% weight fraction of CMBC showed the best procoagulant property.