Themed collection Analyst HOT Articles 2021

The direct modulation of a light-up aptamer that engenders an analyte-specific aptamer-light-up aptamer chimera is readily applicable in any diagnostic assay for a targeted detection.

Detection and recognition of chemical and biological species via sensor electronics are important not only for various sensing applications but also for fundamental scientific understanding.

Complete layout of a centrifugal microfluidic system.

We review the endeavors on the compositional and structural engineering of nanomaterials in the MS characterization of biomolecules in terms of enriching targets, enhancing the ionization efficiency and amplifying the ion signals.

A fluorogenic hydrogen sulfide (H₂S) donor is a fundamental molecular tool used as an exogenous source in biological studies and therapies.

Endogenous H₂O₂ generated by a single HeLa cell that was adhered on the PDA-coated PDMS substrates under 25 mM glucose culture conditions was detected using a home-built photoelectric dual detection platform.

A colorimetric sensor array based on complexometric indicators is proposed for pattern recognition of 14 lanthanide ions.

A fluorescence-tracking system with phenoxazine pseudonucleotides in loops that do not alter iM unfolding enables precise evaluation of ligand-induced changes of iM stability.

A dual-wavelength ECL ratiometry sensing platform was constructed for H₂S detection.

In this work, we employed target-driven assembly of a Mg²⁺-dependent DNAzyme to develop an ultrasensitive electrochemical biosensor for the simultaneous detection of miRNA-21 and miRNA-141.

A new metal-free near-infrared fluorescent probe based on IR-780 is designed and applied for the imaging and detection of carbon monoxide releasing molecule-2 (CORM-2) in paper chips, living cells, zebrafish and mice.

The hexameric and octameric variants of benzene-fused oligo-BODIPYs emit fluorescence in the near-infrared (NIR). Their potential for biophotonics and imaging is reported.

The peroxidase-like activity of Fe-based CPNs was enhanced by Mn doping. The optimum Fe₅Mn-DHTP was devised for colorimetric detection of AA, Cys and GSH by inhibiting TMB chromogenic reaction in presence of H₂O₂via different inhibition mechanisms.

A ratiometric ESIPT probe, FormAFP, has been developed for rapid, selective and sensitive detection of FA via 2-aza-Cope rearrangement. The probe also showed a fast response (<20 min) to both exogenous and endogenous FA in living cells.

We proposed a SERS single bacterial cell imaging strategy and integrated use of intracellular biosynthesized Ag NPs and the MCR model.

A colorimetric sensor based on Cu MOFs with fumaric acid as the ligand shows bifunctional enzyme activities, namely laccase activity at pH = 8 and peroxidase activity at pH = 4.

Reasonable control of the redox states within the catalytic units together with the interconnection degrees of the substrate is of great significance in the modulation of a well-performing transducer.

An anodic-stripping voltammetric immunosensing system was innovatively designed for quantitative screening of C-reactive protein using biomimetic-mineralized bifunctional antibody-Cu₃(PO₄)₂ nanoflowers as the molecular tags.

Schematic of the bifunctional immunosensor based on osmium nano-hydrangeas as a catalytic chromogenic and tinctorial signal output for folic acid detection.

Iodine-modified carbazole derivatives showed two-photon absorption properties, lipid drop specificity and higher anticancer effects.

With various flames and a fast color response, an atomic flame was developed for the highly sensitive POCT of tumor cell-derived exosomes.

Near infrared imaging spectroscopy and chemometrics enable reliable identification of natural and regenerated cellulose textile fibers for chemical recycling.

A multifunction 2MI-P@MSN nanoparticle was constructed for Zn²⁺ sensing and reperfusion injury treatment. This strategy highlights the potential for the detection, absorption and assembly of excess zinc ions simultaneously.

Dried blood spheroid prepared on hydrophobic paper substrate enables the stabilization of red blood cells at room temperature.

A novel electrochemical aptasensor was fabricated for the quantitative detection of β-lactoglobulin, based on target-induced nicking site reconstruction strategy accompanied with nicking enzyme activated hybridization chain reaction amplification.

Highly stable and enhanced aqueous fluorescent CsPbBr₃-mPEG@SiO₂ NCs were synthesized through the synergistic effects of polymer surface ligand and silica encapsulation, and used as a novel “on–off–on” fluorescent probe for detection of Hg²⁺ and GSH.

Trace isotopologue analysis of evaporated liquid water was carried out using spontaneous Raman scattering. A multimode laser diode receiving feedback from a multipass cavity enabled detection of HDO down to D-H concentration ratios as low as 8 ppm.

An electrochemical biosensor system based on a double-tetrahedral DNA framework for ultrasensitive capture, detection and release of circulating tumor cells. The released cells can be recultured for further clinical research.

A visual colorimetric method was developed for the on-site and rapid detection of triacetone triperoxide. This method is applied for analyzing real samples, and a test-paper-based assay was also developed.

A feasible self-assembly near-infrared fluorescence sensor for acid phosphatase detection and cell image.

In situ growth of MnO₂ nanosheets on the surface of BCNPs enabled to realize of specific cell-targeted two-photon fluorescence activation imaging.

The hybrid 3D printed/nitrocellulose-based microfluidic platform analyzed the effect of oxygen gradient on cells with a variety of methods.

An ultrasensitive, rapid, and visual detection platform for Salmonella Typhimurium based on the bio-barcode assay and recombinase polymerase amplification (RPA) coupled with a CRISPR-Cas12a cleavage system is presented.

A fluorescent probe PDPE-PN with prominent sensitivity and specificity, and a fast response towards ONOO⁻ was synthesized and characterized. The probe with good biocompatibility has been successfully applied in cells and zebrafish.

Through the FRET of UiO-66-NH₂ and APBA@ARS with the reaction of copper ions, alizarin red and D-PA, fluorescence and colorimetric platform detection are realized. The mobile phone RGB detection are realized owing to the change of solution color.

A facile one-pot hydrothermal approach for synthesizing water-dispersed nitrogen and sulfur doped carbon dots (NS-CDs) with high luminescence quantum yield and ECL efficiency was explored, using cysteine and tryptophan as precursors.

LTN@AuNCs display peroxidase-like activity in a TMB-H₂O₂ system. Their catalytic activity was enhanced by oxytetracycline. Using the mechanism, a protocol for highly selective and sensitive monitoring of rat serum drug was constructed.

A novel strategy for the POCT of glucose and the simultaneous detection of multiple targets using different fluorescence color cards is developed.

Targeted bivalent anti-Dig antibody as an input to power a directional switch of the DNA structure through a specific binding event with two tethered haptens, rationally integrating with the hybridization chain reaction for signal amplification.

The fluorescence enhancement of red emissive CDs and the casting of its aggregates were realized, and then, applied to the immunoassay of SARS-CoV-2 nucleocapsid proteins.

A sensitive, rapid, and simple procedure for the determination of traces of chromium species in natural and waste waters using a quasi-hydrophobic deep eutectic solvent was developed for the first time.

Nanopores attached to charged species realize the artificial regulation of ion transport by the electrostatic effect in nanoconfines, produce a sensitive ion current signal and play a critical role in nanopore-based analyses.

Duplex SERS-based lateral flow testing for C. diff bacterial infection using new biomarker, SlpA, and ToxB within 20 minutes.

With a larger sample size, 7 mm CMP–NMR can identify and distinguish components in different phases within a softgel capsule of hydroxyzine hydrochloride. The capsule would not fit in a 4 mm probe.

We synthesized a new ATP analogue ATP-NB, and a fluorescent probe TZ-Cy3 based on D–A reaction. Using fluorescent techniques, we established an efficient method for studying the phosphorylation of proteins in solution and in living cells.

A signal-amplified photoelectrochemical immunosensing platform was designed to monitor prostate-specific antigen based on ion-exchange reaction between Zno/CdS and silver nanolabels.

The process of exploring metabolic disturbance induced by fiproles in zebrafish by iEESI-MS.

Digital microfluidic with integrated ITO electrodes patterned on ITO-PET film is used to perform automated immunoassay for H5N1. The better electrochemical performance is achieved using square wave voltammetry.