Contributors to Emerging Investigators 2014
Guest Editors Ulrike Eggert and Michael Washburn introduce this year's Emerging Investigators issue and present the profiles of the contributing authors.
Discovery of protein–RNA networks
We review the latest advances and future challenges in experimental and computational investigation of protein–RNA networks.
Engineering reduced evolutionary potential for synthetic biology
Biological devices can be redesigned to slow evolutionary degradation of their functions by altering how they are encoded in DNA sequences and by engineering host organisms with improved genetic stability.
From cradle to grave: high-throughput studies of aging in model organisms
This review summarizes the development of high-throughput replicative lifespan and longevity aging assays in unicellular and nematode model organisms.
Crosstalk between kinases and Nedd4 family ubiquitin ligases
Understanding the interplay between kinase and E3 ligase signaling pathways will allow better understanding of therapeutically relevant pathways and the design of small molecule therapeutics targeting these pathways.
Improved cyclopropene reporters for probing protein glycosylation
Cyclopropenes have emerged as a new class of bioorthogonal chemical reporters.
Patterning of cells through patterning of biology
For the first time, patterns of cells have been constructed by spatially manipulating native gene expression. This control of expression was effected using light activated RNA interference (LARI), a technique in which knockdown of gene expression is modulated through siRNA modified with light cleavable groups.
One third of dynamic protein expression profiles can be predicted by a simple rate equation
A simple rate equation predicts time-resolved expression for 1/3 of the tested genes and suggest two mutually exclusive modes of regulation.
Peroxisomes are juxtaposed to strategic sites on mitochondria
Peroxisomes are situated in the cell adjacent to specific subdomains of mitochondria such as the ER/Mitochondria contact site or sites enriched for the pyruvate dehydrogenase complex.
The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae
The interactome of the CTD phosphatase Rtr1 is regulated by the CTDK-I subunit Ctk1.
Quantitative phosphoproteomic profiling of PINK1-deficient cells identifies phosphorylation changes in nuclear proteins
A quantitative phosphoproteomic method coupled with siRNA mediated silencing of a kinase associated with Parkinson's disease was applied to measure phosphorylation changes. The results indicate alterations in protein phosphorylation downstream of this kinase, potentially expanding our understanding of its function.
A comprehensive analysis of the Streptococcus pyogenes and human plasma protein interaction network
A schematic figure for the analysis of bacterial interaction protein, 2 different MS methods are used.
Creating functional engineered variants of the single-module non-ribosomal peptide synthetase IndC by T domain exchange
Production of indigoidine can be enhanced by swapping a synthetic T domain into the NRPS IndC.
Method
A red light-controlled synthetic gene expression switch for plant systems
The gene switch can be induced to high expression levels in red light and is inactive in far-red-supplemented white light.
About this collection
A Molecular BioSystems themed issue guest edited by Ulrike Eggert and Michael Washburn showcasing some of the top research from emerging investigators in chemical biology, systems biology and the -omics sciences, the core areas of Molecular BioSystems .