Themed collection Lab on a Chip Emerging Investigators
Slip formation of a high-density droplet array for nucleic acid quantification by digital LAMP with a random-access system
A droplet array SlipChip is presented to compartmentalize fluid into partitions at high density with a simple “Load-Slip” operation, and to perform digital LAMP quantification of SARS-CoV-2 nucleic acids with a random access system.
Microfluidic organ-on-a-chip model of the outer blood–retinal barrier with clinically relevant read-outs for tissue permeability and vascular structure
This organ-on-a-chip device of the outer blood retinal barrier will allow future studies of complex disease mechanisms and treatments of visual disorders using clinically relevant endpoints in vitro.
μCB-seq: microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells
We present a platform for on-chip molecular barcoding that combines high-resolution imaging with genomic analysis, enabling multi-modal phenotypic measurements in single cells.
Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery
We have developed a novel workflow (sdDE-FACS, ingle roplet ouble mulsion FACS) that allows robust production, screening, and sorting of single double emulsion droplets with complete nucleic acid recovery.
A bespoke microfluidic pharmacokinetic compartment model for drug absorption using artificial cell membranes
A new type of pharmacokinetic compartment model using artificial cell membranes that predicts intestinal absorption three times more accurately than the current state of the art.
Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures
To uncover the heterogeneity of cellular populations and multicellular constructs we show on-demand isolation of single mammalian cells and 3D cell cultures by coupling bright-field microdroplet imaging with real-time classification and sorting using convolutional neural networks.
Microfluidic impedance cytometry device with N-shaped electrodes for lateral position measurement of single cells/particles
In this paper, we present an N-shaped electrode-based microfluidic impedance cytometry for the measurement of the lateral position of single cells and particles in continuous flows.
Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples
This handheld sample-to-answer platform combines blood cell separation, viral lysis, and isothermal nucleic acid amplification with capillary fluidics and heating controls to automatically detect HIV from blood samples within 90 minutes.
Magnetic microboats for floating, stiffness tunable, air–liquid interface epithelial cultures
Magnetically anchored microboats that can reliably and rapidly create air–liquid interface culture conditions in substrate-stiffness tunable epithelial cell cultures.
Automated “pick and transfer” of targeted cells using dielectrophoresis
A highly specific and automated device is desired for selective manipulation of single cells. The device in this work picks cells based on their inherent properties using dielectrophoresis and automatically transfers those at a transfer site.
Organ-on-chips made of blood: endothelial progenitor cells from blood reconstitute vascular thromboinflammation in vessel-chips
Organ-on-chips made of blood: personalized medicine with microfluidics.
Measurement of the magnetic susceptibility of subtle paramagnetic solutions using the diamagnetic repulsion of polymer microparticles
The diamagnetic repulsion of polymer microspheres in a microfluidic platform enables the discrimination of magnetic susceptibility of subtle paramagnetic solutions.
3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering
The LEGO® concept is used to build 3D microchannel networks as a 3D μ-electrotransfection system for efficient exchange of nutrition and waste allowing 3D cell growth, while sustaining uniform 3D electric fields during cellular transfection.
Integrated inertial-impedance cytometry for rapid label-free leukocyte isolation and profiling of neutrophil extracellular traps (NETs)
A novel integrated inertial-impedance cytometer for rapid and label-free electrical profiling of neutrophil extracellular trap formation (NETosis).
A miniaturized push–pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain
Measuring neurotransmitters in the brain of living animals is a challenging task, especially when detailed information at high temporal resolution is required.
On-chip phenotypic investigation of combinatory antibiotic effects by generating orthogonal concentration gradients
We propose a microfluidic cell-based drug screening platform to investigate combinatory antibiotic effects by automatically generating concentration combinations.
Resolving protein mixtures using microfluidic diffusional sizing combined with synchrotron radiation circular dichroism
Microfluidic diffusional sizing is combined with synchrotron radiation circular dichroism to resolve the secondary structure of protein mixtures.
Functional TCR T cell screening using single-cell droplet microfluidics
Droplet-based single cell platform allows functional screening and sorting of desirable TCR T cells to accelerate development of adoptive T cell therapies.
An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation
A biomimetic ‘adipose-tissue-on-chip’ integrated with nanoplasmonic biosensors for in situ multiplexed cytokine secretion analysis of obese adipose tissue.
Microfluidic diamagnetic water-in-water droplets: a biocompatible cell encapsulation and manipulation platform
We report a new technique that combines all aqueous droplet microfluidics with diamagnetic manipulation to isolate single-cell encapsulating water-in-water droplets.
Measurement and mitigation of free convection in microfluidic gradient generators
Microfluidic gradient generators are used to study the movement of living cells, lipid vesicles, and colloidal particles in response to spatial variations in their local chemical environment.
A liver-immune coculture array for predicting systemic drug-induced skin sensitization
Recapitulation of liver-immune interactions in a microfluidic compartmentalized coculture array is sufficient to accurately predict systemic drug-induced skin sensitization.
Graphene-based biosensor for on-chip detection of bio-orthogonally labeled proteins to identify the circulating biomarkers of aging during heterochronic parabiosis
Click-A+Chip identifies azido modified proteins with a novel graphene linker that performs copper-free-click chemistry on the chip's surface.
A microfluidic platform for lifelong high-resolution and high throughput imaging of subtle aging phenotypes in C. elegans
Aging produces a number of changes in the neuronal structure and function throughout a variety of organisms.
User-defined local stimulation of live tissue through a movable microfluidic port
A two-component, two-phase microfluidic device provides a movable port for user-selectable local stimulation of living tissue slices.
Microfluidic lung airway-on-a-chip with arrayable suspended gels for studying epithelial and smooth muscle cell interactions
A novel lung airway-on-a-chip system that uses a suspended hydrogel to enable epithelial–matrix–smooth muscle cell interactions.
Flow dynamics control endothelial permeability in a microfluidic vessel bifurcation model
Endothelial barrier function is known to be regulated by a number of molecular mechanisms; however, the role of biomechanical signals associated with blood flow is comparatively less explored.
Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization
A novel portable device combining an aptamer probe and a nanofluidic component was developed, enabling the buffer-free continuous monitoring of small molecules in biological fluids.
Upgrading well plates using open microfluidic patterning
We developed an open microfluidic hydrogel-patterning technology that creates networks of cell culture chambers in well plates for multiculture experiments.
Automated microfluidic platform for systematic studies of colloidal perovskite nanocrystals: towards continuous nano-manufacturing
An automated microfluidic platform enables systematic high-throughput studies of mixing enhancement on the emission band-gap of in-flow synthesized perovskite quantum dots, resulting in kinetically tunable nanocrystals.
A microfluidic device for partial immobilization, chemical exposure and behavioural screening of zebrafish larvae
Movement response of zebrafish to chemicals is of interest in screening studies. We introduce a microfluidic device for partial immobilization, head L-arginine exposure and movement screening of zebrafish larvae under controlled conditions.
Probing blood cell mechanics of hematologic processes at the single micron level
Single-micron PDMS devices provide insight into the biophysical effects that vascular matrices have on platelets, RBCs, and neutrophils.
Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics
Light-based 3D printing of alginate hydrogels using ionic crosslinking enables reversible patterning with controlled degradation.
Image-based feedback and analysis system for digital microfluidics
A new feedback and biological analysis system for digital microfluidics that uses an imaging based setup.
Label-free ferrohydrodynamic cell separation of circulating tumor cells
A size-based ferrohydrodynamic cell separation (FCS) device capable of enriching intact circulating tumor cells with high throughput and high recovery rate.
About this collection
Lab on a Chip is please to present this growing web collection showcasing some of the best research in the field of miniaturisation being conducted by early career scientists. This series is edited by Dino Di Carlo (UCLA, USA), Yoon-Kyoung Cho (UNIST, South Korea) and Piotr Garstecki (IPC PAC, Poland), and aims to highlight up-and-coming researchers in the early stages of the independent careers.
This collection is updated when new Emerging Investigator series papers are published, so keep checking the page for new articles!