Themed collection Analytical Atomic Spectrometry in Biomedical Sciences
X-ray absorption spectroscopy of biological samples. A tutorial
This tutorial presents the principle of X-ray absorption spectroscopy and reviews its application to the characterization of biological samples.
Application of plasma
spectrometry for the analysis of engineered nanoparticles in suspensions and products
Scanning electron micrograph of gold nanoparticles prepared with a low concentration of silver nitrate.
Direct speciation analysis of inorganic elements in single cells using X-ray absorption spectroscopy
This review looks at the advantages and limitations of using micro X-ray absorption spectroscopy for studying speciation of inorganic elements in single cells.
Immunoaffinity assisted LC-ICP-MS—a versatile tool in biomedical research
A novel concept of combining immunochemistry with elemental speciation analysis has potential use for targeted protein and peptide quantification in complex biological matrices.
Sample introduction of single selenized yeast cells (Saccharomyces cerevisiae) by micro droplet generation into an ICP-sector field
mass spectrometer for label-free detection of trace elements
Metals and semi-metals are detected label free in single selenized yeast cells for the first time using an ICP-SFMS.
labelling strategy for integrated ICPMS and LIF for the determination of peptides
A novel dual-labelling strategy with MMA-DOTA-Eu and FITC was developed for the determination of peptides using HPLC-ICPMS and CE-LIF.
Detection of selenoproteins in human cell extracts by laser ablation-ICP MS after separation by polyacrylamide gel electrophoresis and blotting
Laser ablation-ICP MS was optimized for the sensitive detection of selenoproteins in polyacrylamide gel and PVDF membrane after blotting.
Inductively coupled plasma mass spectrometry for determination of total urinary protein with CdTe quantum dots label
A novel methodology for sensitive quantification of total urinary protein by using CdTe quantum dots label and ICP-MS was proposed.
Solid sampling high-resolution continuum source
graphite furnace atomic absorption spectrometry to monitor the biodistribution of gold nanoparticles in mice tissue after intravenous administration
This paper explores the potential of high-resolution continuum source GFAAS for monitoring the biodistribution of Au nanoparticles in animal tissues.
Bioimaging of metals in thin mouse brain section by
laser ablation inductively coupled plasma mass spectrometry: novel online quantification strategy using aqueous standards
A novel solution-based calibration method for quantitative elements imaging in brain tissue sections by LA-ICP-MS is described.
Determination of five
arsenic species in whole blood by liquid chromatography coupled with inductively coupled plasma mass spectrometry
A Vivaspin ultrafiltration device is used to prepare whole blood for LC separation of 5 arsenic species using a TBAH ion pair reagent with ICP-MS detection at m/z 75.
Metal-containing polystyrene beads as standards for mass cytometry
A bivariate plot of mass cytometry results for cells mixed with polymer beads.
Quantitative imaging of element spatial distribution in the brain section of a mouse model of Alzheimer's disease using
synchrotron radiation X-ray fluorescence analysis
The element distributions in a mouse brain are quantitatively determined with SRXRF using Compton scattering normalization.
polymer particles by seeded emulsion polymerization and their characterization by novel ICP-MS detection
A novel method for the analysis of metal-labeled polymer particles by ICP-MS is presented. These particles have relevant surface chemistry for bioconjugation and subsequent use in assays for the detection of biological moieties.
Isotopic analysis of Cu in serum samples for diagnosis of Wilson's disease: a pilot study
Cu in the serum of patients with Wilson's disease shows a lighter isotopic composition than that in the serum of supposedly healthy individuals, thus making Cu isotopic analysis via multi-collector ICP-MS a potential tool for diagnosis of this disease.
Speciation of selenium in cells by HPLC-ICP-MS after (on-chip) magnetic solid phase extraction
Novel methods of (on-chip) magnetic solid phase extraction combined with high performance liquid chromatography-inductively coupled plasma mass spectrometry were proposed for selenium speciation in selenium-enriched yeast cells.
labeling for absolute quantification of intact proteins using label-specific isotope dilution ICP-MS
Absolute quantification of intact proteins labeled with MeCAT-IA using label-specific isotope dilution ICP-MS. Comparison of laser ablation and direct infusion of the mineralized spot for standard and blood serum proteins.
LA-ICP-MS and nHPLC-ESI-LTQ-FT-MS/ MS for the analysis of cisplatin–protein complexes separated by two dimensional gel electrophoresis in biological samples
A methodology for the analysis of Pt–protein complexes in cisplatin-treated biological samples is presented.
Comparison of different chelates for lanthanide
labeling of antibodies and application in a Western blot immunoassay combined with detection by laser ablation (LA-)ICP-MS
In this paper an antibody labeling strategy via MeCAT was developed. The reagent is compared to two other established labeling methods (MAXPAR™, SCN-DOTA) using complex samples in a Western blot immunoassay measured by LA-ICP-MS.
LA-ICP-MS with microarray chip sampling to determine holoceruloplasmin in serum
Determination of holoceruloplasmin in serum using laser ablation ICP-MS with microarray chip sampling.
A pilot study on the use of laser ablation-ICP-mass
spectrometry for assessing/mapping the distribution of a drug and its metabolites across the body compartments of rats
The distribution of a Br-containing anti-TB drug over the organs of rats was visualized and semi-quantitatively assessed via spatially resolved analysis of thin sections using LA-ICP-MS.
Iodine as an elemental marker for imaging of single cells and tissue sections by
laser ablation inductively coupled plasma mass spectrometry
A new LA-ICP-MS based method was developed for single cell imaging and internal standardization for tissue thin sections in LA-ICP-MS.
Mass spectrometric imaging of elements in biological tissues by new BrainMet technique—
laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS)
A novel bioimaging technique, LMD-ICP-MS, was developed to study elemental distribution in tissues with spatial resolution down to 4 μm.
Magnetic quantitative immunoanalysis of carcinoembryonic
antigen by ICP-MS with mercury labels
A novel nonisotopic immunoassay based on mercury labeled magnetic solid phase extraction-MCN-ICP-MS detection was proposed for the quantification of CEA.
Ultrasensitive, selective and simultaneous detection of
cytochrome c and insulin based on immunoassay and aptamer-based bioassay in combination with Au/Ag nanoparticle tagging and ICP-MS detection
An ultrasensitive and specific method for simultaneous determination of cytochrome c (cyt-c) and insulin was developed by combining aptamer-based bioassay and immunoassay, multielement-tagging and ICP-MS.
Quantitative determination of intact free
cisplatin in cell models by LC-ICP-MS
Two complementary LC-ICP-MS methods were successfully applied for quantification of intact, free cisplatin in cytosol of cancer cells. Cellular uptake studies of cisplatin were performed at preclincally relevant experimental conditions (drug exposure in the low µM range).
Reactivity of anticancer metallodrugs with serum
proteins: new insights from size exclusion chromatography-ICP-MS and ESI-MS
The metal binding of anticancer metallodrugs, cisplatin and RAPTA-T, to serum proteins albumin and transferrin was investigated by ICP-MS and ESI-MS.
Accelerator mass spectrometry in the attomolar concentration range for 14C-labeled biologically active compounds in complex matrixes
Accelerator mass spectrometry in combination with column switching and Restricted Access Media column facilitates concentration measurements of drugs in blood down to 450 attomolar.
Isotopic analysis of the metabolically relevant transition metals Cu, Fe and Zn in human blood from vegetarians and omnivores using multi-collector ICP-mass spectrometry
Isotopic analysis of Fe, Cu and Zn from healthy donors using multi-collector ICP-MS reveals the influence of gender on the isotopic composition of Fe and that of diet (vegetarian vs. omnivorous) on the isotopic composition of Zn.
About this collection
Highlighting the achievements in analytical atomic spectrometry and applications of spectroscopy-based bio-analytical technologies in clinics and biology.