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Themed collection Synthetic Biology 2012-2014

11 items
Critical Review

Microbial evolution in vivo and in silico: methods and applications

In this review we focus on the strengths and limitations of in vivo and in silico techniques for studying microbial evolution in the laboratory, and we discuss how these complementary approaches can be integrated in a unifying framework.

Graphical abstract: Microbial evolution in vivo and in silico: methods and applications
From the themed collection: Synthetic Biology 2012-2014
Open Access Paper

Light-responsive control of bacterial gene expression: precise triggering of the lac promoter activity using photocaged IPTG

An optogenetic tool was established allowing for precise, gradual and homogeneous light-triggering of lac-based gene expression in a non-invasive fashion.

Graphical abstract: Light-responsive control of bacterial gene expression: precise triggering of the lac promoter activity using photocaged IPTG
From the themed collection: Synthetic Biology 2012-2014
Paper

Probing subcellular organic hydroperoxide formation via a genetically encoded ratiometric and reversible fluorescent indicator

A ratiometric fluorescent indicator for organic hydroperoxide (OHP) was developed and utilized for the study of endogenous OHP dynamics with subcellular precision.

Graphical abstract: Probing subcellular organic hydroperoxide formation via a genetically encoded ratiometric and reversible fluorescent indicator
From the themed collection: Synthetic Biology 2012-2014
Paper

ERG9 and COQ1 disruption reveals isoprenoids biosynthesis is closely related to mitochondrial function in Saccharomyces cerevisiae

Understanding in vivo regulation of isoprenoid biosynthesis is important for identifying strategies to improve carbon fluxes to desirable endproducts.

Graphical abstract: ERG9 and COQ1 disruption reveals isoprenoids biosynthesis is closely related to mitochondrial function in Saccharomyces cerevisiae
From the themed collection: Synthetic Biology 2012-2014
Paper

Physiological formation of fluorescent and conductive protein microfibers in live fibroblasts upon spontaneous uptake of biocompatible fluorophores

Living fibroblasts spontaneously incorporate a synthetic semiconducting fluorophore and physiologically generate fluorescent conductive microfibers, secreted intracellularly and subsequently extruded.

Graphical abstract: Physiological formation of fluorescent and conductive protein microfibers in live fibroblasts upon spontaneous uptake of biocompatible fluorophores
From the themed collection: Synthetic Biology 2012-2014
Paper

Design principles for generating robust gene expression patterns in dynamic engineered tissues

This work experimentally and computationally evaluates the conditions necessary to establish robust gene expression patterns in engineered tissues in vitro.

Graphical abstract: Design principles for generating robust gene expression patterns in dynamic engineered tissues
From the themed collection: Synthetic Biology 2012-2014
Paper

Experiment and mathematical modeling of gene expression dynamics in a cell-free system

The authors investigate the expression dynamics in a cell-free expression system and establish a minimalistic rate equation model to concurrently fit multiple data sets of GFP synthesis.

Graphical abstract: Experiment and mathematical modeling of gene expression dynamics in a cell-free system
From the themed collection: Synthetic Biology 2012-2014
Paper

A statistical feature of Hurst exponents of essential genes in bacterial genomes

A normal distribution exists for the Hurst exponent of the essential genes in most of the analyzed bacterial genomes.

Graphical abstract: A statistical feature of Hurst exponents of essential genes in bacterial genomes
From the themed collection: Synthetic Biology 2012-2014
Technical Innovation

Real-time optogenetic control of intracellular protein concentration in microbial cell cultures

We have developed a culturing apparatus and feedback control scheme which, in combination with an optogenetic system, allows us to generate defined perturbations in the intracellular concentration of a specific protein in microbial cell culture.

Graphical abstract: Real-time optogenetic control of intracellular protein concentration in microbial cell cultures
From the themed collection: Synthetic Biology 2012-2014
Technical Innovation

Development of siRNA-probes for studying intracellular trafficking of siRNA nanoparticles

This study describes the development of probes for studying the intracellular disassembly kinetics and trafficking of siRNA nanocarriers in a cancer cell line from two different viewpoints; siRNA release and nanoparticle disassembly.

Graphical abstract: Development of siRNA-probes for studying intracellular trafficking of siRNA nanoparticles
From the themed collection: Cancer Nanotechnology
Technical Innovation

Quantitative assessment of Ras over-expression via shotgun deployment of vectors utilizing synthetic promoters

Cells were transduced with Ras transgenes expressed from various synthetic promoters. High resolution dose-response curves relating expression and phenotype were achieved through analysis of cell heterogeneity.

Graphical abstract: Quantitative assessment of Ras over-expression via shotgun deployment of vectors utilizing synthetic promoters
From the themed collection: Synthetic Biology 2012-2014
11 items

About this collection

This collection of articles includes articles published between 2012 and 2014 in Integrative Biology under the theme of Synthetic Biology.

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