Themed collection Analytical Methods HOT Articles 2023

Heavy metal ion contamination poses significant environmental and health risks, necessitating rapid and efficient detection methods.

This review summarizes the photophysical properties of fluorescent probes with the function of targeting cancer receptors and their response mechanisms to biomarkers, as well as their applications in cancer-related biological imaging, and looks forward to the future development direction.

Unpredictable natural disasters, disease outbreaks, climate change, pollution, and war constantly threaten food crop production.

The accurate qualitative and quantitative analysis of PCs and isomers facilitates the discovery of biomarkers for exercise rehabilitation of patients with myocardial infarction.

Polyurea-magnetic hierarchical porous composites show excellent physisorption performance for anionic metabolites in complex matrices with simple preparation, rapid separation, superior stability, and recyclability.

Dimethyl indole Dye IDE was applied to the detection of Lys and Arg level in living cells and zebrafish larvae successfully, and it will be a useful fluorescence indicator for pH changes by Lys and Arg.

Direct detection of dithiocarbamate fungicides (DTCs) has been achieved by SALDI/MS using porous titanium carbide (TiC) ceramic powder as a substrate.

Non-targeted and targeted metabolomic approach method for determination of metabolic profile and chemical composition of guaraná seeds.

SICM is employed as a non-invasive method for mapping the 3D topography of single A549 cells treated with an anticancer drug, to provide information about changes in cell width, height and volume, and monitor membrane blebbing during apoptosis.

Schematic illustration of SERS detection of AD biomarkers using self-assembly Au nanocubes.

Stable and fluorescent ZIF-8 confined CsPbBr₃ perovskite were synthesized to act as a dual functional luminescent probe for the sensing of 4-nitroaniline and rhodamine blue dye. The paper sensor shows visual recognition ability of the analytes.

A novel homogeneous label-free electrochemical aptamer sensor for the detection of progesterone was prepared by combining the omega-like DNA nanostructure, with an isothermal cycling amplification strategy based on the exonuclease III.

To overcome the issues connected to the need of multiple steps in amplification methods for SERS biosensors, we propose a strategy for microRNA detection based on a signal-off mechanism of a labelled molecular beacon immobilized on the SERS substrate.

A PTAD derivatization assisted LC-MS/MS method for the simultaneous determination of seven vitamin D metabolites was established, enabling the quantification of the vitamin D metabolism pathway in various biological samples.

By integrating phenylboronic acid-functionalized fluorescent probe with principal component analysis, it is feasible and easy to discriminate different types of cis-diol containing molecules including nucleosides, sugars and glycoproteins.

An enzyme-free molecularly imprinted sensor with MWCNTs was developed through electrochemical polymerization using L-Glu as a template and 4,6-diaminoresorcinol as a monomer, providing a simple and accurate way for assay of L-Glu in life science.

In this work we report a comparatives study on the design of MIP-based electrochemical sensors for the determination of TOL by PP and TP methods.

A dihydro-benzo[4,5]imidazo[1,2-c]quinazoline-based probe with aggregation-induced ratiometric emission was developed for the ratiometric fluorescent detection of ONOO⁻, which was applied for imaging ONOO⁻ in HeLa cells and zebrafish.

Nitrocellulose (NC) membranes were exploited as synthesis hosts of Prussian blue nanoparticles (PB NPs) to develop a new type of 3D porous peroxidase-mimicking nanozyme pad for colorimetric detection of dopamine (DA) in urine.

Acepromazine (ACP) is a tranquilizer veterinary medication that has emerged as a drug of abuse and is associated with drug-facilitated sexual assaults. Therefore, portable and reliable methods for its detection are of utmost importance.

A novel reverse transcription-accelerated strand exchange amplification (RT-ASEA) method to detect the early biomarker miR-208a of AMI in 15 minutes was developed with high sensitivity, strong specificity and a wide linear range.

Dual-functional metal–organic frameworks were prepared and used for glycoprotein detection based on the boronate-affinity interactions.

A facile visualization method for the tetracycline antibiotics (TCs) detection was developed not only in the laboratory but also in a point-of-care setting using Ag⁺/Tb³⁺@UiO-66-(COOH)₂ as an optical probe.

Nowadays, due to the genetic information carried by nucleic acids, they can serve as a biomarker for the early diagnosis of diseases, including tumors and cardiovascular disease, among others, making genetic testing a hotspot of biomedicine.

The atomic force microscopy was used to analyze the physical properties of cardiomyocytes under the actions of EGFR-TKIs, which provides a new method for screening drugs and exploring the principle of cancer treatment at the cellular level.

Gene-based DNA oligonucleotides are combined with graphene oxide for electrochemical genosensing of neonatal sepsis. The genosensor shows improved, specific and rapid performance and opens a window to developing a miniaturized point-of-care device for neonatal sepsis detection.

A liquid-phase microextraction procedure based on pH-switchable hydrophobic deep eutectic solvents and HPLC-FL analysis. The method was used for the quantitative study of aflatoxin B1 in eight widely consumed cereal samples.

This paper reports the usability of the adsorbent Carbograph 5TD for the analysis of VVOCs in indoor air. It includes the whole validation chain from the determination of recovery, LOD/LOQ and robustness to measurement uncertainty.

A label-free SPCN algorithm detects glioma using Raman spectroscopy. It segments and prunes competitive networks, eliminating the need for outlier threshold estimation or data distribution density. Effective for non-compact manifold data sets.

A rapid and simple multicolor immunosensor was developed for monitoring of vancomycin concentration, and the immunosensor presented good color stability, user friendliness and portability.

An ultrasensitive LFIA based on multilayered fluorescent nanofilm-guided signal amplification was developed for the rapid and quantitative determination of Salmonella typhimurium in complex food samples.

A novel supramolecular recognition-enhanced electrochemical sensing platform based on a 3D-rGO/Pd/β-CD-modified glassy carbon electrode (GCE) is proposed for application in the electrochemical detection of RAC.

The König reaction is newly applied to the simultaneous determination of reduced and oxidized glutathione.

IC-MS based metabolomic analysis identified signature metabolites, which may serve as biomarkers for the clinical detection of T2DM or CHD–T2DM and for the prediction of developing CHD in T2DM patients.

A new ratiometric near-infrared fluorescent probe 3 for detecting ClO⁻ using conjugated 1,8-naphthalimide and dicyanoisophorone with a vinylene linker was reported.

Strategy for detecting human IgG using DNA-functionalized CdTe quantum dots as biosensors.

This manuscript utilizes 2-n-octyl-1-dodecanol as a surface ligand to passivate surface defects of perovskite quantum dots, ultimately improving the performance and stability of the perovskite quantum dots.

The phagocyte's lysosome is the primary site of hypochlorous acid (HOCl) synthesis, and HOCl can be used as a biomarker for osteoarthritis diagnosis and treatment evaluation.

A novel electrochemical sensing platform based on a DNAzyme anchored cell membrane and hybridization chain reaction enzyme-free dual signal amplification strategy was developed for sensitive metal ion detection in the cell microenvironment.

With the increasing prevalence of diabetes mellitus (DM) and diabetic nephropathy (DN), effective treatment is particularly important for the recovery of patients.

This work presents a novel electrochemical sensor based on the third-generation poly(amidoamine) dendrimer (G3 PAMAM)-functionalized multiwalled carbon nanotube-modified screen-printed graphite electrode (SPGE) for the simple and sensitive detection of methotrexate.

The mercury ion (Hg²⁺) has hindered society to some extent due to its high biological toxicity, and a rapid method for Hg²⁺ detection is urgently needed.

Simultaneous and ultrasensitive detection of multiple microRNA (miRNA) biomarkers is an essential precondition for early cancer diagnosis and treatment.

An in vivo multiple labelling strategy using P. pastoris to generate 300 + ¹³C-labelled internal standards was shown to significantly reduce technical variations introduced in typical human lipidomics analyses using >100 samples and >70 h run time.

We show an inexpensive and reliable approach to extend the areas of application of paper-based analytical devices for protein quantification.

Here we describe the double resonator piezoelectric cytometry to simultaneously measure the H9C2 cardiomyocytes’ generated force and viscoelasticity towards the goal of studying the systolic and diastolic function of the cardiovascular system.

23-Nordeoxycholic acid was deployed to probe metabolism pathways of endogenous bile acids, and metabolite identification was achieved by applying the rule that C₂₃–CH₂ defect quantitatively modifies both MS² spectrum and retention time features.

An arylboronic acid with the capability of Hg²⁺ recognition response was coordinated to Eu-MOF to construct a novel target-responsive dual-emission fluorescence nanoprobe for the ratiometric sensing detection of Hg²⁺ in environmental water.

Illustration of the fabrication of NiCo-MOF/GO and its application in glucose determination.

We present a low-cost and portable point-of-care lipid profile measurement device based on centrifugal microfluidics principles. We validate our device with venous and capillary bloods.

Sugammadex (SUG) is a synthetically modified γ-cyclodextrin derivative used in hospitals after surgeries to reverse the neuromuscular blockade induced by rocuronium or vecuronium.

A DNA polymerase-powered self-propelled DNA walking strategy is proposed for dual-signal, amplified electrochemical detection of nucleic acids.

Colloidal GA-capped PNC ethanol solution shows a continuous PL shift once aqueous Cl⁻ with different concentrations is added. This fluorescent sensor shows a wide linear detection range (2–200 mM) of Cl⁻, short response time (∼1 s), and low limit of detection (1.82 mM).

The detection of explosives, drugs and precursor chemicals from data derived using colorimetric sensing experiments can be done effectively using machine learning methods.

Recently, the growing number of medical interventions has led to the risk of invasive candidiasis.

Two Fe(III)/Eu(III) Salophen complex-based optical sensors for determination of organophosphorus pesticide monocrotophos in camellia oil and tap water.

Butanone-enhanced vacuum ultraviolet photoionization ion mobility spectrometry provides a new method for monitoring the spatial distribution of atmospheric ammonia and supporting the air quality assessment.

This sensor uses I_Fc as the conformation change signals and I_MB as the internal reference signal. AgNWs@ZIF-8 is used to generate an internal reference signal. The sensor shows a range of 20 nM to 9 μM with a detection limit of 10 nM (S/N = 3).

The interaction mechanism of CDs with metal ions was explored by using fluorescence kinetics.

LC-Raman spectrometry, which is enabled with the aid of a vertical flow method, is applied to an analysis of sugar contents in a honey sample. Experimental apparatus, data analysis method, and error estimation are discussed.

A peptide-based silicon nanowire array sensor for the specific detection of TNT was developed, and the effect of the biointerfacing linker was investigated.

Iron(III) thenoyltrifluoroacetonate (Fe(TTA))₃ was used as a mobile-phase soluble and nanostructured sorbent for solvent-assisted dispersive solid phase extraction for the enrichment of bisphenols from surface waters prior to HPLC analysis.

TD GC-MS can be used as an additional tool for determining polyurethane degradation products, closing the low-molecular mass gap.

The procedure developed, based on an ultrasound probe assisted extraction followed by DFATR-MIR analysis, offers a fast, simple and efficient way for the determination of cocaine camouflaged in different types of solid samples.

A ratiometric fluorescent probe for Cu²⁺ based on Tb(III)-functionalized UiO-66-type MOFs has been synthesized via a facile post-synthetic modification method by employing mixed linkers containing terephthalic acid and 2,6-pyridinedicarboxylic acid.

In this study, a data augmentation algorithm GKIM based on the Gaussian kernel density is proposed for the data augmentation of normal tissue spectra. It can effectively solve the class imbalance problem.

A novel atmospheric pressure glow discharge (APGD) microplasma system was applied for the determination of Zn, Mg, Ca, Na, and K in selected beverages by optical emission spectrometry (OES), with a simplified sample preparation procedure.

The fabrication and sensing process of Met-CDs.

We aim to develop a protocol for efficiently analysing breast microcalcifications and their microenvironment in detail by combining O-PTIR and Raman spectroscopy at the same time, the same location and the same high resolution (0.5 μm).

In this study, a new method for the point-of-care testing of thiodiglycol (TDG) using molecularly imprinted polymer (MIP)-coated electrospun nanofiber membranes is reported.