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Fluorescent SAM analogues for methyltransferase based DNA labeling

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Abstract

In this work, the preparation of new S-adenosyl-L-methionine (SAM) analogues for sequence specific DNA labeling is evaluated. These non-natural analogues, comprising cysteine rather than the natural homolog, were obtained in near quantitative conversions from readily available starting materials without relying on using an excess amount of labor intensive molecules. The synthetic strategy was used to generate fluorescent cofactors, with colours spanning the whole visible spectrum, and their applicability in methyltransferase based optical mapping is shown.

Graphical abstract: Fluorescent SAM analogues for methyltransferase based DNA labeling

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Article information


Submitted
15 Nov 2019
Accepted
22 Jan 2020
First published
06 Feb 2020

This article is Open Access

Chem. Commun., 2020, Advance Article
Article type
Communication

Fluorescent SAM analogues for methyltransferase based DNA labeling

V. Goyvaerts, S. Van Snick, L. D'Huys, R. Vitale, M. Helmer Lauer, S. Wang, V. Leen, W. Dehaen and J. Hofkens, Chem. Commun., 2020, Advance Article , DOI: 10.1039/C9CC08938A

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