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Issue 13, 2015
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Flow induced dispersion analysis rapidly quantifies proteins in human plasma samples

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Abstract

Rapid and sensitive quantification of protein based biomarkers and drugs is a substantial challenge in diagnostics and biopharmaceutical drug development. Current technologies, such as ELISA, are characterized by being slow (hours), requiring relatively large amounts of sample and being subject to cumbersome and expensive assay development. In this work a new approach for quantification based on changes in diffusivity is presented. The apparent diffusivity of an indicator molecule interacting with the protein of interest is determined by Taylor Dispersion Analysis (TDA) in a hydrodynamic flow system. In the presence of the analyte the apparent diffusivity of the indicator changes due to complexation. This change in diffusivity is used to quantify the analyte. This approach, termed Flow Induced Dispersion Analysis (FIDA), is characterized by being fast (minutes), selective (quantification is possible in a blood plasma matrix), fully automated, and being subject to a simple assay development. FIDA is demonstrated for quantification of the protein Human Serum Albumin (HSA) in human plasma as well as for quantification of an antibody against HSA. The sensitivity of the FIDA assay depends on the indicator-analyte dissociation constant which in favourable cases is in the sub-nanomolar to picomolar range for antibody–antigen interactions.

Graphical abstract: Flow induced dispersion analysis rapidly quantifies proteins in human plasma samples

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Supplementary files

Article information


Submitted
10 Apr 2015
Accepted
27 May 2015
First published
27 May 2015

This article is Open Access

Analyst, 2015,140, 4365-4369
Article type
Communication
Author version available

Flow induced dispersion analysis rapidly quantifies proteins in human plasma samples

N. N. Poulsen, N. Z. Andersen, J. Østergaard, G. Zhuang, N. J. Petersen and H. Jensen, Analyst, 2015, 140, 4365
DOI: 10.1039/C5AN00697J

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