A microfabrication-free nanoliter droplet array for nucleic acid detection combined with isothermal amplification

Abstract

A nanoliter droplet array based on a hydrophilic–hydrophobic patterned chip is developed without using microfabrication technology. Combined with the isothermal amplification technology, it has been applied to perform nucleic acid detection, showing excellent specificity and sensitivity. As a versatile platform, it is used to detect three gene targets successfully.