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Issue 96, 2014
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Investigating fluorescent dyes in fluorescence-assisted screenings

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Abstract

Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads. The introduction of zwitterionic dyes significantly reduced the unwanted interactions, which sheds light upon using the right fluorescent probe for acquisition of reliable results in various fluorescence-assisted applications.

Graphical abstract: Investigating fluorescent dyes in fluorescence-assisted screenings

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Article information


Submitted
05 Sep 2014
Accepted
13 Oct 2014
First published
23 Oct 2014

Chem. Commun., 2014,50, 15220-15223
Article type
Communication

Investigating fluorescent dyes in fluorescence-assisted screenings

J. Jee, J. Lim, H. Hyun, J. Oon, Y. S. Ong, C. Massif, Y. Chang, H. S. Choi and S. S. Lee, Chem. Commun., 2014, 50, 15220
DOI: 10.1039/C4CC07008A

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