Rie
Mukai
*ab,
Takashi
Fukuda
a,
Asami
Ohnishi
a,
Takeshi
Nikawa
c,
Mutsuki
Furusawa
a and
Junji
Terao
a
aDepartment of Food Science, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, Japan
bDepartment of Food Science, Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1, Minamijosan-jima, Tokushima 770-8513, Japan. E-mail: rmukai@tokushima-.ac.jp; Fax: +81-88-656-8029; Tel: +81-88-656-9917
cDepartment of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, Tokushima, Japan
First published on 9th December 2020
In this study, we evaluated the food matrix effects of chocolate on the absorption of green tea catechins (GTCs), (−)-epicatechin (EC), (−)-epigallocatechin (EGC), (−)-epicatechin gallate (ECg), and (−)-epigallocatechin gallate (EGCg), in five healthy 22-year-old women. In the single-intake experiment, the plasma concentrations of ECg (P < 0.05, at 1.5 h) and EGCg (P < 0.05, at 6 h) but not those of EC and EGC were reduced by the chocolate matrix. Regardless of the chocolate matrix, ECg and EGCg were mainly present as their aglycones in the plasma, whereas EGC and EC were found mostly as conjugated metabolites. After daily intake of GTCs mixed with chocolate for 14 days followed by overnight fasting, ECg but not EGCg was detected in the plasma. To compare the plasma profiles of ECg and EGCg, a mixture containing approximately equal amounts of ECg and EGCg was administered to nine rats for 14 days. Following treatment and overnight food deprivation, the plasma content of ECg was higher than that of EGCg. After a single injection of the same mixture in seven rats, ECg levels were higher than those of EGCg, and a greater amount of conjugated metabolites of ECg than those of EGCg was detected in the plasma 10 h after administration. In conclusion, the chocolate matrix affects the plasma profiles of GTCs, particularly ECg. ECg appears to persist in the plasma for a longer period, regardless of the chocolate matrix.
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Fig. 1 Chemical structures of the catechins assessed in this study. EC: (−)-epicatechin; EGC: (−)-epigallocatechin; ECg: (−)-epicatechin gallate; EGCg: (−)-epigallocatechin gallate. |
The bioavailability of GTCs has been demonstrated in animal and human studies. After being consumed, GTCs undergo conjugation or methylation in the intestinal mucosa and liver,9 with their plasma concentrations peaking within 90 to 180 min. These metabolites pass into enterohepatic circulation.10,11 In addition, GTCs reaching the large intestine are converted into several species of organic acids by the gut microflora.11 Pharmacokinetic studies have found that all catechin components are cleared from the plasma within 24 h and excreted in the urine within 48 h of consumption.10,12 Thus, to experience the beneficial effects of GTCs, it may be necessary to ingest them regularly. GTCs are mainly ingested by beverages, but since the stability of GTCs in liquids is reported as low,13 we considered using solid foods as the food matrix to GTCs. If it could be ingested GTCs in food other than beverages, more people can achieve the health benefits of catechins. For example, it can be supplied for elderly people with chronic disease at risk of aspiration, and astronauts who exposing oxidative stress during space flight because of the portability.14 Chocolate is eaten all over the world, and since it can suppress the bitterness of catechins, it was applied as the food matrix in the current study. In addition, since chocolate contains EC oligomers (procyanidins) that would affect the bioavailability of GTEs, in this study, we examined the effect of cacao polyphenols on the bioavailability of GTCs.
We aimed to investigate the impact of chocolate as a food matrix on the bioavailability of GTCs; in particular, plasma profiles reflecting conjugation metabolism and dynamic changes to GTC plasma concentrations were assessed.
The single-intake study (Exp. I) and continuous-intake study (Exp. II) were carried out separately.
Exp. I Healthy female volunteers aged 22 years (BMI = 16.2–20.2) were enrolled (n = 5). Three kinds of test meals were prepared: 375 mg of Sunphenon BG-5 (BG-5) in 200 mL of water, 20 g of chocolate (Cho), or 20 g of chocolate with 375 mg BG-5 (Cho + BG-5). The catechin composition of each test meal is listed in Table 1. All subjects took the same test meal at the same time. The experimental order was Cho + BG-5, Cho, and BG-5. The washout period between the test meal was 7–8 weeks. The protocol of Exp. I was described in a previous report.15 Briefly, all subjects were asked to avoid catechin-rich foods and drinks for one day before the experiment. After an overnight food fast, blood samples were collected into heparinised tubes just before test meal intake, then 1.5 and 6 h after consumption.
BG-5 | BG-5 + Cho | Cho | |
---|---|---|---|
BG-5, Sunphenon BG-5: tea catechin mix for human experiments. Cho, chocolate | |||
(−)-Epicatechin (EC) | 117 | 132 | 14 |
(−)-Epicatechin gallate (ECg) | 25 | 25 | n.d. |
(−)-Epigallocatechin (EGC) | 351 | 351 | n.d. |
(−)-Epigallocatechin gallate (EGCg) | 221 | 221 | n.d. |
Exp. II Healthy female volunteers aged 22 years (BMI = 16.2–21.6) were enrolled (n = 5). Four of them were the same women as Exp. I, one of them was changed to another woman (there was no health problem, but personal reason). The study subjects ingested test meal once a day at 12:00 pm for 14 days. All subjects took the same test meal at the same time. The experimental order was Cho + BG-5, and Cho. The washout period between the test meal was 5 weeks. All subjects were instructed to consume their usual diet, except for catechin-rich foods and drinks, during the experimental period. Fasting blood samples were collected into heparinised tubes at day 0 (just before the first intake), 15 (24 h after final intake), and 22 (after a seven-day washout period) after overnight fasting. To control the baseline plasma levels of catechins, the same feeding restrictions as in Exp. I was applied the day before each blood collection.
The plasma was separated by centrifugation at 9000g for 10 min at 4 °C, then stored at −30 °C until analysis.
Male Wistar rats (Japan SLC) were housed in a room maintained at 23 ± 1 °C with a 12 h light–dark cycle. The animals were allowed free access to a commercial diet (AIN-93 M, Oriental Yeast Co., Ltd Tokyo, Japan) and water. Before test meal administration, they were deprived of food for 24 h but had free access to water. Nine rats (three 15-, 19-, and 27-week-old, respectively) were included in the daily injection study (Exp. III). The catechin composition of each test substance is listed in Table 2. Sunphenon 20 ECg-OP (ECg-OP) was dissolved in water and administered to the rats once a day for 14 days through a gastric feeding tube (100 mg per kg body weight). Blood samples were collected on day 0 (just before the first dose) and 14, following 24 h food deprivation. Seven rats (three eight- and four 19-week-old) were included in the single-injection study (Exp. IV). ECg-OP was dissolved in water and administered to the rats via a gastric feeding tube (200 mg per kg body weight). Blood samples were drawn just before injection, then at 1, 4, 8, 10, 18, and 24 h after injection. The plasma was separated by centrifugation at 9000g for 10 min at 4 °C, then stored at −30 °C until analysis.
Experiment III (100 mg Sunphenon 20 ECg-OP per kg body weight) | Experiment IV (200 mg Sunphenon 20 ECg-OP per kg body weight) | |
---|---|---|
(−)-Epicatechin (EC) | 12.4 | 24.8 |
(−)-Epicatechin gallate (ECg) | 76.4 | 152.8 |
(−)-Epigallocatechin (EGC) | 7.5 | 15.0 |
(−)-Epigallocatechin gallate (EGCg) | 60.9 | 121.8 |
The same detection system, equipped with a Cadenza CD-C18 column (75 × 4.6 mm; Imtakt, Kyoto, Japan) was used for rat plasma. The mobile phase was 1% acetic acid and acetonitrile (90/10, v/v) and the flow rate was 1.0 mL min−1.
In the single-intake experiment, the plasma concentrations of ECg and EGCg were lowered by mixing them with chocolate. The absorption of GTCs is affected by the food matrix.16–18 The bioavailability of EGCg and ECg, but not that of EC and EGC, is reduced by skim milk, caseinase, and soy protein.16 The proteins contained in these foods are suggested to impair the absorption of EGCg and ECg. The chocolate used in this study contained 7.6% protein, which may have inhibited intestinal absorption of ECg and EGCg. The fat content of chocolate (33.8% in the current study) also seems to affect the intestinal absorption of GTCs. The logP values (a measure of hydrophobicity) of EGC, EC, EGCg, and ECg were −0.50, +0.11, +0.39 and +1.06, respectively.19 The gallate moiety on the C ring increases the hydrophobicity of the molecule. It is assumed that the higher the hydrophobicity of the catechin, the slower its release from the chocolate matrix. Thus, EGC and EC, which are hydrophilic, may be more easily released from the food matrix and enter the digestive juice more readily than hydrophobic EGCg and ECg.
EGCg and ECg mostly existed as aglycones in human plasma (Fig. 3). The ratio of aglycone of EGCg to total EGCg was significantly lower after BG-5 + Cho consumption than after BG-5 consumption (P = 0.033).
According to previous reports, GTCs with a galloyl moiety at the 3-position mostly exist as aglycones in the plasma.20,21 Our results obtained 1.5 h after test food intake may reflect conjugation metabolism in enterocytes and hepatocytes (Fig. 2). Chocolate increased the levels of conjugation metabolites of EGCg (Fig. 3). To our knowledge, this is the first report to demonstrate that food components can enhance the conjugation metabolism of ingested GTCs.
After 14 days of daily test food intake, the plasma concentration of ECg was increased in women consuming BG-5 + Cho, and it was significantly higher than in those consuming chocolate alone (P = 0.034) (Fig. 4). EC present in chocolate increased plasma EC levels in the Cho group. However, since the EC content of BG5 was 10 times higher than that of chocolate, the plasma EC concentration was higher in the Cho + BG5 group than in the Cho group on day 15. Chocolate had no effect on the plasma concentration of EGC. The plasma content of EGCg was markedly lower than that of ECg, and it did not change significantly during the study period. Plasma levels of all catechins returned to baseline after a seven-day washout period (day 22).
The results of our human experiment imply that excessive accumulation of ECg resulting from daily intake can be avoided because plasma ECg levels returned to baseline after a seven-day washout period (day 22). Our data provide evidence that daily consumption of GTCs is required to achieve the desired health-promoting effects, such as anti-atherosclerotic,8 antioxidative,22,23 and nephroprotective24 effects. As reported by Kawai et al.8 ECg was detected in foamy macrophages in human atherosclerotic aorta by using specific antibodies to ECg, where it was shown to inhibit the expression of CD36, a class B scavenger receptor implicated in the development of atherosclerosis. Maintenance of an appropriate level of ECg in the blood through daily consumption of GTCs could facilitate ECg transport to atherosclerotic lesions.
The results of our single-intake experiment in women showed that 1.5 h after test food consumption, the maximum plasma levels of ECg and EGCg were lowered by chocolate, whereas those of EC and EGC were comparable between the BG-5 and BG-5 + Cho groups. In contrast, after 14 days of daily test food intake, the plasma concentration of ECg was higher in women ingesting BG-5 + Cho than in those consuming BG-5 alone. These data suggest that chocolate delays intestinal absorption of ECg after a single intake, without reducing the total amount of absorbed ECg. Although the plasma content of ECg was much lower than that of EGCg at 1.5 h after a single BG-5 intake (Fig. 2), after 14 days of daily BG-5 intake followed by an 18 h fast, ECg concentration exceeded that of EGCg (Fig. 4). These data suggest that ECg could be a preferred food factor that exerts beneficial effects on vascular endothelium function upon daily intake of tea catechins. Previous studies on catechin bioavailability demonstrated that the clearance of ingested ECg from plasma is slower than that of EGC and EGCg.9,22 In the study by Van Amelsvoort et al., the plasma concentrations of EGC and EGCg returned to basal levels within 24 h of test meal ingestion, whereas that of ECg remained.22 Therefore, ECg may be eliminated more slowly and persist in the blood for a longer period than EGCg.
Approximately 15% of the polyphenols in chocolate are procyanidins, such as procyanidin B2 [epicatechin-(4β-8)-epicatechin], procyanidin C1 (trimer), and cinnamtannin A2 (tetramer).25 The chocolate used in our clinical investigation contained 144 mg polyphenols per 20 g (determined using the Folin-Ciocalteu method; data not shown), thus, it was estimated to contain approximately 20 mg procyanidins per 20 g. Among them, procyanidin B2 is partly absorbed as EC and its conjugated/methylated metabolites.26,27 Therefore, EC in the plasma of women consuming chocolate-mixed BG-5 or chocolate alone may partly originate from procyanidins, such as procyanidin B2. However, the impact of procyanidins on the absorption of monomeric catechins is not yet fully understood. Further studies are required to clarify the interactions between catechins and procyanidins, and the effects of procyanidins on GTC absorption when chocolate is used as the food matrix.
In these clinical studies, it is necessary to monitor the ingestion during the clinical test, so we had to employ students who belonged to the university as volunteers. This was why only 22-year-old women were selected as the study subjects, and it was the limit of this study. However, the effect of chocolate on the bioavailability of GTCs was properly analyzed because all subjects ingested their respective test meals reliably. In the future study, it will be necessary to employ subjects with different genders, ages, races, health conditions, and eating habits, and to increase the number of subjects to clarify the food matrix effect of chocolate.
After a single gastric injection of ECg-OP, the maximum plasma concentration of total ECg (conjugates and aglycones combined) was higher than that of total EGCg (Fig. 6). The aglycone data followed the same trend until 8 h after ingestion. The concentration of ECg was significantly higher than that of EGCg between 4 and 24 h after injection. From 10 h after injection onwards, the plasma content of total ECg and total EGCg increased, whereas that of their respective aglycones decreased over time.
The plasma concentration of total ECg and EGCg peaked at 1.5 h after test substance injection, before steadily decreasing until the 4 h mark. Between 4 and 8 h after ingestion, both ECg and EGCg entered various peripheral tissues, such as the liver, re-entered the duodenum via enterohepatic circulation, or were eliminated by the kidneys. From 10 h after ingestion onwards, the plasma level of total ECg (aglycone and conjugated metabolites combined) but not that of the aglycone of ECg increased again, and its concentration was significantly higher than that of EGCg. Therefore, most ECg was conjugated metabolites, which were likely reabsorbed via enterohepatic circulation and/or re-entered circulation from peripheral tissues. During the latter process, ECg may undergo conjugation metabolism several times, and the metabolite profile of ECg present in blood may become more complex, according to previous studies.9 In contrast, the EGCg level did not change appreciably after the 10 h mark. ECg in the body was responsible for its elevated plasma levels after 14 days of daily GTC intake in women. ECg seems to undergo more efficient enterohepatic recycling than EGCg, which likely underlies its longer persistence in the plasma during regular intake.In addition, chocolate may influence the plasma ECg profile of humans.
To avoid stress from blood sampling several times in humans, rats were applied to a time-course study to confirm the longer persistence of ECg in the blood. Comparing the metabolite profile of tea catechins between rats and humans, glucuronides mainly existed in rat plasma, however not only glucuronides but also sulfates existed in human plasma.28,29 In this study, the conjugated metabolites were hydrolysed by the deconjugation reaction, and then the total concentration (subtotal of aglycone and conjugated metabolites) were mainly analyzed. Therefore, it was not possible to confirm the effect of the difference in metabolite profile on the plasma concentration of each catechin. In the pharmacokinetics study of tea catechins, common results are known to be obtained from both rats and humans. For example, in both cases, the maximum concentration of ingested catechins appears in 1–2 hours, disappears from the blood in about 24 hours, and most of the gallate-type catechins existed as aglycones.9,12,30,31 Therefore, the plasma concentration of each catechin in this study can be extrapolated pharmacokinetics appropriately.
This study was funded by the Commission of Space Science and Technology Promotion, MEXT (2013) (J. T. and T. N.), and LOTTE Co., Ltd (Tokyo, Japan, 2012-2014) (J. T. and T. N.). These financial supports did not have a grant number. All funders played no role in the design, analysis, or writing of this article.
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