Cristina
Sissa
*a,
Anna
Painelli
a,
Francesca
Terenziani
a,
Massimo
Trotta
b and
Roberta
Ragni
*c
aDipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124, Parma, Italy. E-mail: cristina.sissa@unipr.it
bCNR-IPCF, Institute for Physical and Chemical Processes, Bari unit, Via Orabona 4, 70125 Bari, Italy
cDipartimento di Chimica, Università degli Studi di Bari “Aldo Moro”, I-, via Orabona 4, 70126 Bari, Italy. E-mail: roberta.ragni@uniba.it
First published on 10th December 2019
Aminoalkyl-substituted heptamethine cyanine dyes are characterized by a large Stokes shift, an uncommon feature for cyanine molecules yet very promising for their application as fluorescent probes in bioimaging and as light harvesting antennas in biohybrid systems for solar energy conversion. The origin of this photophysical feature has not been adequately explored so far, and a combined experimental and theoretical work is herein provided to shed light on the role played by the central aminoalkyl substituent bound to the heptamethine cyanine backbone in defining the unusual properties of the dye. The spectra recorded in solvents of different polarities point to a marginal role of the medium in the definition of the Stokes shift, which conversely can be ascribed to the relaxation of the molecular geometry upon photoexcitation. This hypothesis is supported by an extensive theoretical investigation of the ground and excited states of the dye. TD-DFT results on the aminoalkyl-substituted dye and its unsubstituted precursor demonstrate a very similar cyanine-like structure for both molecules in the relaxed excited state. Conversely, in the ground state the amino substitution disrupts the conjugation in the polymethine chain, leading to a broken-symmetry, non-planar structure.
Heptamethine cyanines are promising for bioapplications; their spectra are in fact located in the near-infrared region, where the ability to penetrate biological tissues is the highest and the autofluorescence is marginal. Unfortunately, two main drawbacks hinder their use as fluorescent bioprobes: poor photostability and a very small Stokes shift (about 0.05 eV), the latter causing fluorescence self-absorption and quenching. Photostability is improved by introducing a chlorocyclohexenyl ring in the central position of the polymethine chain,12 and this also enhances the fluorescence quantum yield and provides a reactive site. This reactive site has been exploited to introduce a series of substituents on the central cyclohexenyl ring via nucleophilic substitution of the chlorine atom with amines,13 thiols,14–16 alcohols17,18 and phenyls.19,20 In particular, amino-substituted heptamethine cyanine dyes obtained by substitution of the chlorine atom show a huge Stokes shift (larger than 0.3 eV),13 maintaining or even improving the fluorescence properties typical of heptamethine dyes. These molecules have been exploited as fluorescent probes for bioimaging21,22 and in artificial light harvesting antennas for solar energy conversion.23–25
The mechanism at the basis of the spectroscopic effects observed upon substitution of the chlorine atom with an amino-alkyl group has not been fully addressed in the literature. Different research groups proposed an interplay between localized excitation and intermolecular charge transfer, but clear experimental evidence for this interplay is missing.13,23 The ion-pairing effect was also invoked for heptamethine cyanines showing different behaviours according to the counterion and/or the polarity of the solvent.19,26 The nitrogen group in the central core definitely affects the ground-state electronic configuration, favouring the presence of the charge on the central position.27,28 However, a thorough investigation of the first excited state and of its relaxation after vertical excitation is necessary to understand the origin of the large Stokes shift.
In this paper, a comparison between two cyanine dyes with different spectral properties is presented (Scheme 1): IR-780 is a commercially available dye bearing a chlorocyclohexenyl ring in the central position of the heptamethine cyanine structure, showing negligible (≈0.04 eV) Stokes shift; conversely HCy2, the amino-alkyl functionalized analogue obtained by nucleophilic substitution of the chlorine atom in IR-780 shows a large (≈0.3 eV) Stokes shift. HCy2 has been selected and synthesized in the frame of studies on biohybrid systems for solar energy conversion based on the covalent bioconjugation of organic light harvesting antennas to photoenzymes.23–25 The specific octyl chain of HCy2 was chosen as a suitable spacer between the chromophore and the protein, in order to prevent the sterical hindrance of HCy2 on the photoenzyme surface, thus preserving the enzymatic photoactivity.29,30
A combined experimental and theoretical work sheds light on the origin of this large Stokes shift: the nitrogen atom linked to the cyclohexenyl ring strongly affects the conjugation of the polymethine chain, leading to a broken-symmetry ground state. On the opposite, the symmetry is partially restored in the first excited state (upon relaxation), leading to a large structural re-arrangement of the molecule that explains the observed large Stokes shift.
Fig. 1 Absorption (continuous lines) and emission (dashed lines) spectra of IR-780 and HCy2 in DCM (black lines) and DMSO (red lines). |
Molecule | Solvent | λ absmax [nm] | λ emmax [nm] | Stokes shift [eV] |
---|---|---|---|---|
IR-780 | DCM | 790 | 808 | 0.03 |
DMSO | 796 | 816 | 0.04 | |
HCy2 | DCM | 665 | 768 | 0.25 |
DMSO | 636 | 773 | 0.35 |
Fluorescence anisotropy spectra provide details about the relative orientation of transition dipole moments of absorption and emission processes and therefore can be exploited to assess the possible contribution of different electronic excitations to the same absorption band, useful information when dealing with broad absorption spectra as for HCy2. The fluorescence excitation anisotropy spectrum recorded for HCy2 in glassy ethanol (EtOH) at 77 K is shown in Fig. 2. The fluorescence anisotropy signal is flat within the lowest excitation band, suggesting that a single electronic transition is responsible for the absorption band. The measured anisotropy is close to 0.3, meaning that transition dipole moments of emission and absorption form an angle close to 24°. The Stokes shift measured at 77 K is somewhat reduced compared to that at room temperature (Fig. 2), but is still sizeable. This residual Stokes shift measured in the glassy solvent can be safely attributed to the relaxation of the molecular geometry after photoexcitation.
Fig. 2 Top panel: Absorption and emission of HCy2 at room temperature in EtOH. Bottom panel: Excitation, emission and fluorescence excitation anisotropy spectra of HCy2 in EtOH at 77 K. |
The selected ground state properties of IR-780 and HCy2 are summarised in Table 2 (the dihedral angles δ1 and δ2 and the bond length alternations, BLA1 and BLA2 are defined in Fig. 3). The dihedral angles δ1 and δ2 provide information about the planarity of the molecule. The ground state of IR-780 has a planar structure, with δ1 and δ2 close to 180°. On the opposite, HCy2 is distinctly non-planar in the ground state, with both δ1 and δ2 appreciably different from 180°. Bond length alternations, BLA1/2 are also calculated: a small BLA implies a good electronic delocalization along the chain (strong cyanine character), while a large BLA implies alternating single and double bonds. Moreover, dissimilar BLAs in the two molecular arms clearly point to symmetry breaking. In IR-780, BLA1 and BLA2 are both close to 0, meaning that all bonds in the polymethine chain have a very similar character, intermediate between single and double bonds. BLA1 and BLA2 are larger in HCy2, with the bonds relevant to 1, 3, 6 and 8 having a predominant character of double bonds, and 2, 4, 5 and 7 having a predominant single-bond character. Furthermore, BLA1 differs from BLA2, pointing to a broken-symmetry ground state. The asymmetry is also confirmed by the length of the two central bonds of the polymethine chain (4 and 5) that in HCy2 are non-equivalent and significantly longer than in IR-780.
Molecule | Solvent | δ 1 (°) | δ 2 (°) | BLA1 (Å) | BLA2 (Å) | 4 (Å) | 5 (Å) |
---|---|---|---|---|---|---|---|
IR-780 (GS) | DCM | 174 | −174 | 0.015 | 0.015 | 1.408 | 1.408 |
DMSO | 174 | −174 | 0.015 | 0.015 | 1.407 | 1.407 | |
HCy2 (GS) | DCM | −162 | −154 | 0.110 | 0.086 | 1.444 | 1.434 |
DMSO | −162 | −154 | 0.113 | 0.089 | 1.445 | 1.436 | |
IR-780 (1EX) | DCM | 174 | −174 | 0.013 | 0.013 | 1.414 | 1.414 |
DMSO | 174 | −174 | 0.011 | 0.011 | 1.414 | 1.414 | |
HCy2 (1EX) | DCM | −173 | −170 | 0.025 | 0.024 | 1.420 | 1.422 |
DMSO | −174 | −171 | 0.023 | 0.022 | 1.419 | 1.422 |
To discuss the charge distribution in the molecules, we have arbitrarily divided each molecule into three fragments (left, central and right), as defined in Fig. 3. The cumulative charges, calculated for the three fragments as the sum of Hirshfeld atomic charges, are given in Table 3. For IR-780, the charge is symmetrically distributed over the two molecular arms, and is mainly localized on the peripheral groups of the molecule. The structural asymmetry of HCy2 in the ground state shows up with a non-symmetric charge distribution in the two arms. Moreover, in HCy2 the charge residing in the central fragment is comparable to that in the peripheral subunits, in agreement with previous results by Pascal et al.27 The dyes are not soluble in non-polar solvents, but calculations run in toluene (see the ESI†) confirm that the solvent polarity marginally affects the ground state properties of either dye.
Molecule | Solvent | Left | Central | Right |
---|---|---|---|---|
IR-780 (GS) | DCM | 0.476 | 0.048 | 0.476 |
DMSO | 0.464 | 0.072 | 0.464 | |
HCy2 (GS) | DCM | 0.360 | 0.250 | 0.390 |
DMSO | 0.322 | 0.322 | 0.356 | |
IR-780 (1EX-Vertical) | DCM | 0.465 | 0.070 | 0.465 |
DMSO | 0.439 | 0.122 | 0.439 | |
HCy2 (1EX-Vertical) | DCM | 0.405 | 0.188 | 0.407 |
DMSO | 0.372 | 0.254 | 0.373 | |
IR-780 (1EX-Relaxed) | DCM | 0.464 | 0.072 | 0.464 |
DMSO | 0.441 | 0.118 | 0.441 | |
HCy2 (1EX-Relaxed) | DCM | 0.430 | 0.139 | 0.431 |
DMSO | 0.410 | 0.188 | 0.402 |
Table 4 summarizes the TD-DFT results for the lowest energy vertical excitation. The second electronic transition is calculated at much larger energies for both molecules, with an energy gap between the first and the second transition larger than 1.5 eV for IR-780 and 0.9 eV for HCy2. Therefore, the second electronic transition is irrelevant to the present discussion. The calculated transition energies are blue-shifted compared to the experiment, a well-known issue for cyanine dyes.34 The TD-DFT calculations in toluene (see the ESI†) demonstrate negligible effects of solvent polarity on the absorption spectra of IR-780. The calculated absorption spectra in HCy2 slightly blue shift as the solvent polarity increases, in agreement with the experimental data. The lowest transition is well described as a HOMO–LUMO transition, and is delocalized over the whole molecule, as shown in Fig. 4. The HOMO and LUMO of IR-780 are perfectly symmetric, while the symmetry is lost in the orbitals of HCy2. The decrease of symmetry has important consequences in the energy positioning of the orbitals: the energy gap between the HOMO and LUMO is increased in HCy2, leading to a blue shift of the absorption band.
Molecule | Solvent | Absorption [nm/eV] | Emission [nm/eV] | Stokes shift [eV] |
---|---|---|---|---|
IR-780 | DCM | 556/2.23 (H−L 0.69; H−1 → L+1 −0.13) | 574/2.16 (H−L 0.69; H−1 → L+1 0.13) | 0.07 |
DMSO | 556/2.23 (H−L 0.69; H−1 → L+1 0.13) | 577/2.15 (H−L 0.69; H−1 → L+1 −0.12) | 0.08 | |
HCy2 | DCM | 482/2.57 (H−L 0.67; H−1 → L+1 −0.16) | 554/2.24 (H−L 0.69; H−1 → L+1 0.13) | 0.33 |
DMSO | 481/2.58 (H−L 0.67; H−1 → L+1 −0.16) | 556/2.23 (H−L 0.69; H−1 → L+1 0.13) | 0.35 |
Fig. 4 The HOMO and LUMO of IR-780 (left) and HCy2 (right) in DMSO (equilibrium ground-state geometry). |
The geometry of the first excited state was optimized using the same level of theory adopted for the ground state and accounting for the solvent in the corrected linear response theory of PCM. The solvent polarity barely affects the emission energy of either IR-780 or HCy2 (Table 4). The main qualitative difference between the two molecules concerns the Stokes shift, which, in excellent agreement with the experiment, is predicted to be very small for IR-780 (less than 0.1 eV) and becomes large (more than 0.3 eV) in HCy2.
We are in the position to clarify the origin of the large Stokes shift observed for HCy2. The role of solvent polarity is marginal, since comparable Stokes shifts are calculated in DCM and DMSO (see Table 4) as well as in toluene (see the ESI†). The origin of Stokes shift is therefore safely ascribed to the geometry relaxation in the excited state.
Tables 2 and 3 summarize the computational results about the geometry and charge distribution in the vertical and relaxed excited states of the two molecules. Important structural changes upon excitation are observed for HCy2: (1) the dihedral angles δ1 and δ2 increase upon excitation and, becoming close to 180°, point to planarization of the molecule in the excited state; (2) both BLA1 and BLA2 significantly reduce and become comparable; and (3) the bond-lengths 4 and 5 also reduce and become comparable. Overall, the first excited state of HCy2 has a nearly planar and symmetric structure with an almost equalized bond-length, as a typical cyanine-like system. These structural changes in the excited state with respect to the ground state are responsible for the large observed Stokes shift in HCy2. On the opposite, no significant structural changes are observed in the first excited state of IR-780 with respect to the ground state, reflecting a small Stokes shift.
In HCy2, the charge distribution also undergoes significant changes in the excited state, as reported in Table 3. After excitation (the vertical excited state), the charge redistributes almost symmetrically over the two arms, and a significant charge depletion is observed in the central core of the molecule with respect to the ground state. The charge depletion in the central core of the molecule further increases after solvent relaxation, and in the relaxed excited state, charge distributions of HCy2 and IR-780 are very similar.
Quite interestingly, while important spectroscopic effects are observed upon substituting the central chlorine with amino-groups, the Stokes shift remains small in the case of substitution with thiols or alcohols.14,16–18 These experimental observations suggest that only the nitrogen atom has the ability to conjugate with the polymethine chain. From DFT calculations, in fact, the central C–N bond shows an intermediate character between the single and double bond (the C–N bond length in the ground state of the modified HCy2 is ≈1.35 Å). On the opposite, the DFT calculations on analogous structures with –OCH3 and –SCH3 substituents predict a predominant character of the single bond (see the ESI†).
The absorption spectra of HCy2 are distinctly different from those of typical cyanine-dyes like IR-780. The absorption spectrum is strongly blue-shifted (more than 0.3 eV) and much broader compared to that of IR-780. On the opposite, the emission of HCy2 has a similar bandshape with respect to IR-780, and is only marginally blue-shifted (around 0.07 eV), resulting in a very large Stokes shift for HCy2. Long polymethine dyes are prone to symmetry breaking in the ground state:8 broken-symmetry cyanines show very broad absorption bands and a large Stokes shift, spectral features that are also recognized in HCy2. However, symmetry breaking in long polymethine dyes is typically induced by polar solvation, while absorption and fluorescence spectra of HCy2 are marginally affected by the solvent.
Quantum chemical calculations suggest that in the ground state of HCy2, the central C–N bond has an intermediate character between the single and double bonds. The presence of such a strong bond affects the geometry and conjugation of the polymethine chain: the ground state structure of HCy2 is non-planar and non-symmetric. In the ground state, the charge is preferentially localized in the central part of the molecule,27 and the charge distribution is not symmetric between the two molecular arms. Overall, looking at the ground state properties, HCy2 cannot be described as a cyanine-dye, but upon excitation, the molecule becomes a typical cyanine-dye: the relaxed excited state has an almost planar and symmetric structure, whose quasi-symmetric charge distribution resembles the charge distribution in IR-780.
The origin of the Stokes shift of amino-substituted polymethine dyes is ascribed to the important structural changes occurring after photoexcitation. Symmetry breaking plays a key role in the observation of the large Stokes shift in symmetric structures: as experimentally observed and rationalized in terms of essential-state models,8,11 large Stokes shifts (strongly dependent on solvent polarity) are observed when symmetry breaking occurs in the first excited state or in the ground state.8,10 Here a different type of ground-state symmetry breaking is observed that originates from a structural distortion due to the strong conjugation of the amino group with the polymethine chain: this structural distortion does not depend on solvent polarity, and is almost lost in the first excited state, giving broad and blue-shifted absorption spectra, and cyanine-like narrow emission spectra.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c9cp05473a |
This journal is © the Owner Societies 2020 |