Liang
Qin
,
Li-Xian
Lin
,
Zhi-Ping
Fang
,
Shui-Ping
Yang
,
Gui-Hua
Qiu
,
Jin-Xiang
Chen
* and
Wen-Hua
Chen
*
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. E-mail: jxchen@smu.edu.cn; whchen@smu.edu.cn
First published on 19th October 2015
We herein report a water-stable 3D dysprosium-based metal–organic framework (MOF) that can non-covalently interact with probe ss-DNA. The formed system can serve as an effective fluorescence sensing platform for the detection of complementary Ebolavirus RNA sequences with the detection limit of 160 pM.
At present, one of the effective methods for the detection of Ebolavirus nucleic acid is polymerase chain reaction (PCR), including reverse transcription-PCR (RT-PCR) and real-time quantitative PCR (Q-PCR).4 This PCR method has received considerable attention because of its rapid analysis.5 However, its further applications suffer from high cost, risk of contamination and false negative results.6 Therefore, it is in urgent need to develop more effective approaches for the detection of Ebolavirus RNA.
Metal–organic frameworks (MOFs), readily built from metal ions and organic ligands, are a new class of inorganic materials. The host backbones are composed of metal cation centers and functional organic linkers to produce one, two, or three dimensional extended coordination networks7 with unique properties, such as sorption,8 magnetism,9 luminescence10 and catalysis.11 Recently, MOFs have been reported as sensing platforms for biomolecules, including proteins, nucleic acids and antibodies.12 To the best of our knowledge, however, there is no report on using MOF platforms for sensing Ebolavirus RNA.
Herein we report a new 3D dysprosium (Dy) MOF based on a zwitterionic carboxylate ligand N-carboxymethyl-(3,5-dicarboxyl)pyridinium bromide (H3CmdcpBr),13 that is {[Dy(Cmdcp)(H2O)3](NO3)·2H2O}n (1), and its high selectivity and sensitivity for the fluorescence detection of Ebolavirus RNA sequences.
Compound 1 was synthesized in 62% yield from the reaction of Dy(NO3)3·6H2O with deprotonated pyridinium carboxylate in water at room temperature. Compound 1 is moisture and water stable. The powder X-ray diffraction (PXRD) pattern of a fresh powder of 1 immersed in H2O for one month is in agreement with that of the simulated one, indicating its bulk phase purity and water stability (Fig. 1). Thermogravimetric analysis (TGA) indicates that the as-synthesized sample of 1 is stable up to 280 °C (Fig. S1, ESI†).
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Fig. 1 PXRD patterns of compound 1 showing agreement between the simulated (a), synthesized (b) and fresh powder of 1 immersed in H2O for one month (c). |
Compound 1 crystallizes in the monoclinic space group P21/n and each asymmetric unit consists of one [Dy(Cmdcp)(H2O)3]+ cation, one NO3− anion and two free water molecules. As shown in Fig. 2a, the Cmdcp ligand is located on the inversion center and coordinates to one Dy ion in a chelating fashion and to four Dy ions in a bridging bidentate coordination fashion. Each Dy center is coordinated by three water molecules, one chelating and four monodentate carboxylates from five Cmdcp ligands (Fig. 2b), thereby forming a monocapped square-antiprism coordination geometry as shown in Fig. 2c. The Cmdcp ligand thus acts as a five-connected node, whereas the Dy center also acts as a five-connected node, leading to a 3D framework (Fig. 2d). The 3D MOF exhibits chair-type pore shapes with tessellating H2O and free NO3− anions on the pore surface (Fig. S2, ESI†).
Because of these unique structural features, compound 1 should, in principle, be able to strongly interact with fluorophore carboxyfluorescein (FAM)-labeled single-stranded DNA (ss-DNA) through π–π stacking, hydrogen bonding and electrostatic interactions, and then quench the fluorescence of FAM.14 To test this, we chose FAM-5′-CATGTGTCCAGCTGATTGCC-3′ as a probe ss-DNA (P-DNA). This sequence is complementary to the part of the conservative sequence from Ebolavirus RNA. As shown in Fig. 3a, addition of compound 1 led to a decrease in the fluorescence intensity of P-DNA, suggesting that compound 1 can efficiently quench the fluorescence of P-DNA, possibly via the formation of a non-covalent complex (P-DNA@1 hereafter). It is found that the fluorescence intensity decreases gradually until the concentration of compound 1 reaches 11 μM, indicating saturation of the adsorption of P-DNA. The quenching efficiency (QE, %) of P-DNA is 60%, which was calculated according to the equation QE% = (F0 − FM)/F0 × 100%, where FM and F0 are the fluorescence intensities at 518 nm in the presence and absence of compound 1, respectively. In this case, the molar ratio of compound 1 to P-DNA is 220:
1. This high ratio may be due to the long chain of FAM-5′-CATGTGTCCAGCTGATTGCC-3′ bearing a large FAM tag with a diameter of 9.4 Å, which prevents P-DNA to enter the one-dimensional channels with an approximately 8.3 × 3.3 Å2 pore aperture. Thus the pore of compound 1 cannot adsorb P-DNA efficiently.
In order to gain further insight into the quenching properties of compound 1, we measured the quenching efficiency of Dy(NO3)3 and H3CmdcpBr toward P-DNA. The data are shown in Fig. S3 (ESI†) and indicate that H3CmdcpBr results in negligible fluorescence quenching (QE% = 9.4%). In contrast, the presence of Dy(NO3)3 led to significant quenching (QE% = 85%) with a saturation concentration of 32 μM. These results suggest that Dy(NO3)3 is also efficient in quenching the fluorescence of P-DNA. This may be a consequence of the intercalation of Dy3+ ions into the base pairs of P-DNA and the electrostatic binding with the phosphate backbones,15 triggering a photoinduced electron transfer (PET) process from FAM to Dy3+.16
When the selected Ebolavirus RNA sequences are complementary to the P-DNA sequence, they can form a stable DNA/RNA hybrid duplex. Thus, the addition of complementary Ebolavirus RNA sequences to the P-DNA@1 or P-DNA@Dy(NO3)3 system may compel P-DNA away from compound 1 or Dy(NO3)3, leading to fluorescence regeneration. In such a sense, the P-DNA@1 and P-DNA@Dy(NO3)3 systems can serve as sensing platforms for Ebolavirus RNA. This hypothesis was tested by the fluorescence regeneration induced by the addition of Ebolavirus RNA sequences (5′-GGCAAUCAGUUGGACACAUG-3′, T0), a complementary target RNA to P-DNA. As shown in Fig. 3b, the fluorescence is recovered upon addition of T0 to the P-DNA@1 system. Interestingly, the fluorescence recovery of the P-DNA@1 system was found to increase with incubation time and remained unchanged after 120 min (Fig. S4, ESI†). This long recovery time is probably due to the adsorption of P-DNA by the pores of 1 by its non-FAM end as shown in Scheme 1. The big steric hindrance of FAM shields the P-DNA re-combination with the Ebolavirus RNA and makes the hybridization difficult. In addition, saturation in the fluorescence recovery was observed at the concentration of 50 nM. Under these conditions, the linear relationship between the fluorescence intensity and the concentration of T0 (inset of Fig. 3b) gave the detection limit of 160 pM, which was calculated from 3δb/slope (δb = standard deviation of five blank measurements). The fluorescence recovery efficiency (RE) was 0.65, which was calculated using the formula RE = FT/FM − 1, wherein FT and FM are the fluorescence intensities at 518 nm in the presence and the absence of T0, respectively. However, the fluorescence of the P-DNA@Dy(NO3)3 system could not be recovered, presumably due to the strong interaction between Dy3+ ions and P-DNA (Fig. S5, ESI†).15 These results suggest that it is the unique structure of compound 1 that plays an essential role in fluorescence quenching and regeneration. Specifically, compound 1 forms a complex with P-DNA through multiple non-covalent interactions, in which Dy3+ ions play an important role in quenching the fluorescence of P-DNA. Such moderate interactions enable P-DNA to be released from the complex by forming a stable DNA/RNA hybrid duplex with T0, leading to fluorescence regeneration.17 Thus, the P-DNA@1 system can be used as a highly effective fluorescent sensor for Ebolavirus RNA sequences.
In order to investigate the selective sensing ability of the P-DNA@1 system, we chose two different target RNAs, that is, one base pair mutated RNA T1 (5′-GGCCAUCAGUUGGACACAUG-3′) and non-specific T2 (5′-GACCAACGTUUAGTCTCAUG-3′) to hybridize with P-DNA in the P-DNA@1 system. Under the same conditions, the RE is 0.07 for T1 and 0.02 for T2, respectively (Fig. 3c). In addition, T0 shows much higher concentration-dependent fluorescence recovery than T1 and T2 (Fig. S6, ESI†). These results indicate that the P-DNA@1 system can function as a highly selective sensing platform for the detection of Ebolavirus RNA sequences in vitro.
In order to further evaluate the selectivity of this sensing system, we conduct the experiment under acidic (pH 6.4) and basic (pH 8.0) conditions. As shown in Table 1 and Fig. S7–S12 (ESI†), under both conditions, compound 1 is efficient in quenching the fluorescence of P-DNA with QE% of 65% (pH 6.4) and 77% (pH 8.0), respectively. Meanwhile, the fluorescence of the P-DNA@1 system could be recovered in the presence of T0 with an RE of 0.27 (pH 6.4) and 0.15 (pH 8.0). Under the same conditions, the RE is only 0.06 for T1 and 0.01 for T2 at pH 6.4, 0.03 for T1 and 0.02 for T2 at pH 8.0, indicating that the P-DNA@1 system can function as a highly selective sensing platform for the detection of Ebolavirus RNA at both pH 6.4 and 8.0. The RE values at pH 6.4 (0.27) and 8.0 (0.15) are much lower than that at pH 7.4 (0.65). This may be because of that at pH 6.4, FAM exists in neutral or monoanion mode, while at pH 7.4, FAM exists in dianion or trianion mode18 and exhibits stronger repulsion with NO3− in the pores of 1. Thus the fluorescence of P-DNA is easier to recover at pH 7.4. While at pH 8.0, part of Dy3+ in the MOF 1 may form Dy(OH)3 particles,19 and the fluorescence may be difficult to recover due to the strong interaction between Dy3+ ions and P-DNA as we discussed above.
pH | Q E (%) | R E for T0 | R E for T1 | R E for T2 |
---|---|---|---|---|
6.4 | 65 | 0.27 | 0.06 | 0.01 |
7.4 | 60 | 0.65 | 0.07 | 0.02 |
8.0 | 77 | 0.15 | 0.03 | 0.02 |
The above results may be rationalized from the unique structure of compound 1. Firstly, compound 1 contains aromatic rings, positively charged pyridinium and Dy3+ cation centers, free H2O and NO3− on the pore surface. The zeta potential of +9.0 mV indicates that compound 1 is positively charged.20 Thus, it is reasonable to deduce that compound 1 can absorb P-DNA through electrostatic, π-stacking and/or hydrogen-bonding interactions to form the P-DNA@1 complex,21 and thus quench the fluorescence of FAM, in which Dy3+ may play a critical role in triggering photoinduced electron transfer (PET) from FAM to Dy3+ (Scheme 1).16
Secondly, the channel size of compound 1 may play a critical role in effectively distinguishing ss-DNA from the DNA/RNA duplex.22 Because of the large cross-sectional areas and relatively rigid structures, the formed DNA/RNA duplex may not easily enter the pore of compound 1. In contrast, the smaller cross-sectional areas may make it easier for P-DNA to enter the pore and closely interact with the surface of compound 1. Thirdly, the most important reason is that compound 1 may have less affinity for duplex DNA/RNA because of the absence of unpaired bases and the rigid conformation of duplex DNA/RNA.21b P-DNA is conformationally flexible and should interact with the surface of compound 1 more strongly than rigid duplex DNA/RNA. Therefore, the hybridization of T0 with absorbed P-DNA would lead to the release of labeled dye with the formed duplex DNA/RNA to the solution, thus resulting in the recovery of fluorescence (Scheme 1).12f This is supported by the changes in the fluorescence anisotropy (FA) of P-DNA and P-DNA@T0 (hybrid duplex DNA/RNA) before and after the addition of compound 1. It is known that FA can be a measure for the rotational motion-related factors of DNA,23 and thus provide a means to judge whether P-DNA and P-DNA@T0 are attached to the surface of compound 1. As shown in Fig. 3d, the addition of compound 1 to P-DNA leads to an increase in the FA by a factor of 4.8, whereas has negligible influence on P-DNA@T0. This result reveals the much stronger interaction of compound 1 with P-DNA than with hybrid duplex DNA/RNA.
In summary, we have successfully synthesized and characterized a moisture and water-stable MOF 1 of dysprosium(III) with a zwitterionic carboxylate. Compound 1 can form electrostatic, π-stacking and/or hydrogen bonding interactions with P-DNA. The formed P-DNA@1 system can be used as an effective, selective and fluorescent sensing platform for the detection of Ebolavirus RNA sequences. These findings may provide guidance for the synthesis of more metal–zwitterionic carboxylate compounds with potential applications in the early diagnosis of Ebolavirus disease.
We are grateful to the financial support from the Guangdong Provincial Department of Science and Technology of China (2015A010105016) and Guangdong Provincial Natural Science Foundation of China (2015A030313284), the National Natural Science Foundation of China (No. 21102070), the Program for Pearl River New Stars of Science and Technology in Guangzhou (No. 2011J2200071) and Southern Medical University.
Footnote |
† Electronic supplementary information (ESI) available: General synthesis, X-ray crystallography, crystallographic data, selected bond distances and angles, thermogravimetric analysis and the 3D structure of 1. Ebolavirus RNA detection and fluorescence anisotropy experiments. The fluorescence quenching efficiency of P-DNA by compound 1, H3CmdcpBr and Dy(NO3)3 (pH 7.4). The fluorescence intensity of the P-DNA@1 system in the presence of T0 at varying incubation times (pH 7.4). The fluorescence spectra of the P-DNA@Dy(NO3)3 system incubated with T0 at varying concentrations (pH 7.4). The fluorescence spectra of P-DNA incubated with compound 1 at varying concentrations (pH 6.4 and 8.0). The fluorescence spectra of the P-DNA@1 system incubated with T0 at varying concentrations (pH 6.4 and 8.0). The fluorescence recovery efficiency of the P-DNA@1 system by T0, T1 and T2 at varying concentrations (pH 6.4, 7.4 and 8.0). CCDC 1417644. For ESI and crystallographic data in CIF format or other electronic format see DOI: 10.1039/c5cc06697b |
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