Ben-Xing Zhanga,
Gang-Yi Zhanga,
Hui Gao*a,
Shao-Hui Wua,
Jian-Hua Chena and
Xiao-Long Lib
aSchool of Physical Science and Technology, Key Laboratory for Magnetism and Magnetic Materials of Ministry of Education, Lanzhou University, Lanzhou 730000, P. R. China. E-mail: hope@lzu.edu.cn; Fax: +86 931 8913554; Tel: +86 931 8912772
bShanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, P. R. China
First published on 22nd December 2014
Strongly fluorescent polyethylene glycol passivated nitrogen-doped carbon dots (P-NCDs) were prepared through a one-step facile hydrothermal process using glutamic acid as the precursor. The passivated nitrogen-doped carbon dots with an average size of 6.5 nm showed strong blue luminescence when excited with ultraviolet light. Excitation wavelength dependent photoluminescence was observed in the obtained P-NCDs, and the relationship between the excitation and emission wavelength could be formulated as a linear function. The P-NCDs exhibit noticeable enhancement of photoluminescence after passivation and the quantum yield was as high as 18.7%. The P-NCDs also exhibited luminescence resistance to heavy metal ions to some extent. The excellent comprehensive properties enable the P-NCDs to have potential applications in detecting the polluted environment and other bio-imaging fields.
To date, heteroatoms such as nitrogen (N),13 sulfur (S),14 and boron (B)15 have been incorporated into the framework of the carbon nanoparticles successfully. It's especially watchable that conspicuous progresses have been made on N doped CDs (NCDs). N-doping has been proved to modify the electron density and improve the chemical activities and photic properties.11 Similar radius between the atoms of C and N (the radius of C: 0.77 Å, N: 0.75 Å) facilitates N atoms to be incorporated into the C matrix. Furthermore, strong covalent bond between C and N brings about long-term stability in the NCDs. Glutamic acid (GA) is the benign source of C and N because of its cheapness and abundance. Recently, glutamic acid (GA) was used as the precursor for synthesizing nitrogen doped carbon nanoparticles by heating process.16,17 GA is a green and very cheap amino acid, which plays an important role in the metabolism of human body. And it contains abundant functional groups such as carboxylic acid and amino, which would react effectively in the formation of NCDs. However, it is hard to control the heating process accurately and this would affect the homogeneity of the final products. In addition to that, the prepared CDs only handled with doping process possess the insufficient luminescence.18 Sun's group19 demonstrated that the surface passivation makes a significant contribution to bright PL and the passivated defects are crucial to excitation energy traps. Up to now, polyethylene glycol (PEG),15 polyethylenimine,16 ethylenediamine,17 1-hexadecylamine18 et al. have been confirmed to act as the agent of passivators. Normally, the passivation process was another independent procedure after the preparation of NCDs.19–21
In our research, we adopted a facile one-step hydrothermal method of preparing PEG6000-passivation NCDs (P-NCDs) using GA as the precursor. The choose of PEG6000 is due to its high molecular weight, which would contain its polymeric structure under the hydrothermal condition for further passivated the NCDs. Besides that, PEG has good water solubility and should be beneficial to biocompatibility. The results indicated that the existence of carbonization and amidation in the reaction process. The obtained P-NCDs exhibit significant enhancement in the brightness of PL (the quantum efficiency of ca. 18.7%.) when compared with passivation-free NCDs as well as the resistance to heavy metal ions.
PbCl2, Cd(NO3)2·4H2O, and CuCl2·2H2O were selected as the sources of heavy metal ions and each ion solution was confected to the concentration of 0.01 mol l−1. In all ion batches, 0.5 ml of metal ion solution was blended into 10 ml P-NCDs solution to form a homogeneous solution, respectively. The blank batch was diluted with 0.5 ml ultra pure water too. The corresponding PL spectra were recorded at the excitation of 350 nm after 0.5 h.
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Fig. 1 (a) Schematic formation of P-NCDs. (b) Details about the preliminary amidation of GA. The digital photos of NCDs (c) and P-NCDs (d) under sunlight and 365 nm, respectively. |
The as prepared P-NCDs were polydisperse and the mean diameter was 6.5 nm, as shown in Fig. 2a and b. Size distribution analysis (Gaussian curve) demonstrated that the range of size was 7.0 ± 3.0 nm. Although the polydisperse was an inevitable problem when the teflon-lined autoclave cooled to room temperature gradually and slowly, 90% of particles were included in a narrow range. The high resolution TEM image of P-NCDs revealed the distinct lattice structure in Fig. 2c, indicating their highly crystalline. The lattice space of 0.2096 nm and 0.2103 nm were calculated from the detailed line profile of L1 and L2 (Fig. 2d and e), respectively. The average distance for P-NCDs was 0.21 nm, in accordance with the previous reports, which was attributed to the (100) of graphene (in-plane).14,22 In order to investigate the size effect of passivation, we also compared the TEM images of P-NCDs and NCDs (Fig. S1, ESI†) and found that there was a slight increasing for average size of dots (from 5.9 nm to 6.5 nm) after passivation, which were due to the coverage of PEG6000 on the surface of NCDs.
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Fig. 2 (a) TEM image of P-NCDs. (b) Size distribution and histogram. (c) High resolution TEM image of P-NCDs. High profile along corresponding line L1 (d) and L2 (e). |
XPS could provide us the composition of elements and their chemical state. The atoms of C, N, and O in P-NCDs were detected at the peaks of ca. 284 eV, 400 eV, and 533 eV, respectively, as shown in Fig. 3a. The composition of P-NCDs was calculated to 42.4 at.% of C, 9.3 at.% of N, and 48.3 at.% of O according to the full scan spectra. A high resolution XPS for C 1s, N 1s and O 1s were given in Fig. 3b–d. As shown in the fine C spectrum, distinct peaks at 284.5 eV, 285.7 eV, 287.3 eV, and 288.2 eV were attributed to the bonds of graphite-like sp2 C, CN, C–N (overlapped with C
O) and COOH, respectively.23–25 Thus, the N doping of carbon dots could be confirmed by the bonds between C and N. The high-resolution of N spectrum could be divided into two main peaks: 398.7 eV of pyridinic N and 399.9 eV of lactam and imide N.26 And it was proposed that amino-groups would react with carboxylic acid groups and form the lactam N or imide N in the structure of P-NCDs, which was in agreement with our possible reaction process (Fig. 1b). High resolution image indicated that O atoms mainly presented in two groups: C
O and C–OH,27 in line with the conclusions from the analysis of C spectra. FT-IR spectra of P-NCDs, GA and PEG6000 were shown in Fig. S2 (ESI†). Comparing curve P-NCDs with others, the stretching vibrations of N–H were observed at ca. 3300 cm−1. Besides that, the strong peaks from 1630 to 1740 cm−1 resulted from the carboxylic acid C
O and amide C
O stretches in the P-NCDs. C
C stretching was formed by the appearance of 1435 cm−1, indicating the carbonization of GA into the graphite-like carbon dots structure.28 These data are in well agreement with XPS results. We examined the Raman spectra of the samples (Fig. S3, ESI†), and the fluorescence background of Raman spectra was removed through an intelligent algorithm.29 The ratio of disorder (D) band and graphite (G) band represented the degree of disorder in C material and the level of the defect amount. As shown in Fig. S3,† the ratio of ID/IG was higher after the passivation, implying more defects introduced into the NCDs.
Excitation wavelength dependent PL were observed in the P-NCDs (Fig. 4a and b). Emission wavelength shifted from 415 nm (purple) to 500 nm (green) when the excitation wavelength escalated from 320 nm to 420 nm with the strongest emission intensity at 435 nm. The phenomena could be due to the emissions from different size NCDs as well as the different emissive sites on each passivated NCDs.30 Interestingly, the relationship between the excitation and emission wavelength could be formulated as the linear fitting function: y = ax + b. Using this precise function, we could reckon the excitation wavelength in order to gain specific color in applications. In addition to that, it was observed great enhancement of PL intensity in P-NCDs compared with passivation-free NCDs (Fig. 4d). Previous literature19 noted that this phenomenon was put down to the changes of the surface state. Passivated defects were introduced through the passivation process, which would bring more excitation energy traps. Therefore, more photons of excitation beam could be captured and transformed into emission irradiation with the Stokes shift of ca. 90 nm. At the same time, it was also a noteworthy fact that there was a slight increasing (from 425 nm to 450 nm) of the emission wavelength when PEG was adhered on the surface. According to the results in the TEM data, the passivation process enlarged the size of P-NCDs. The larger size of fluorescent nanodots could conduce the red shift of the emission peak.2 Therefore, it is reasonable that the size effect of passivation accounts for this slight shift. In addition, the quantum yield of P-NCDs was calculated to ca. 18.7% (under 350 nm) using quinine sulfate as the reference, and the lifetime curve was complying with tri-exponential decay with the lifetime of 5.5 ns (Fig. S4 ESI†). In Fig. 4c, a wide peak around 350 nm was observed in the PL excitation (PLE) spectra, which conforms to the conclusion that the strongest emission intensity is obtained with an excitation wavelength of 350 nm. Characteristic peaks are illustrated in UV-vis absorption curve (Fig. S5, ESI†). Apart from the edge absorption, peaks at ca. 230 nm, 280 nm, and 325 nm are assigned to π → π* of conjugated diene, π → π* of CN, and n → π* of C
O, respectively.31 The energy levels are displayed in Fig. S6 (ESI†). The introduction of N atoms brought about the new energy level. This N energy level was regarded as “bridge” which is essential to the absorption at 280 nm.14
Interestingly, as shown in Fig. 5 the luminescence of P-NCDs exhibited resistance to heavy metal ions (Pb2+, Cd3+ and Cu2+) to some extent. Previous studies3 indicated that the presence of some heavy metal ions could lead to the luminescence quenching of CDs and the probable mechanism was the formation of complexes between ions and hydroxyl groups. And in our research, the P-NCDs exhibited less effectively quenching properties for the heavy metal ions compared with that of CDs, which would be related to the hydrothermal process. As mentioned before, the hydroxyl groups from –COOH could react mostly with the –NH2 groups. When introducing the heavy ions into the P-NCDs, the possibility of the complexing reactions would decrease and lead to the above surprising phenomenon.
In brief, we have developed a one-step facile method for preparing P-NCDs. The obtained product exhibited brighter photoluminescence than those without passivated. The carbonization and amidation would occur in the complex reactions. Excitation wavelength dependent PL was observed and the relationship between excitation and the corresponding emission wavelength could be formulated as the linear fitting function. In addition, the luminescence of P-NCDs exhibit resistance to heavy metal ions to some extent.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c4ra08869g |
This journal is © The Royal Society of Chemistry 2015 |