Markus
Hollmann
*a,
Jens
Boertz‡
a,
Elke
Dopp
b,
Joerg
Hippler
a and
Alfred Vitalis
Hirner
a
aInstitute of Environmental Analytical Chemistry, University of Duisburg-Essen, Universitaetsstrasse 3-5, 45141 Essen, Germany. E-mail: markus_hollmann@uni-due.de; Fax: +49 (0)201 1833951; Tel: +49 (0)201 1833238
bInstitute of Hygiene and Occupational Medicine, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany. E-mail: elke.dopp@uni-due.de; Fax: +49 (0)201 7234546; Tel: + 49( 0)201 7234578
First published on 6th October 2009
Methylation of metal(loid)s by bacteria or even mammals is a well known process that can lead to increased toxicity for humans. Nevertheless, reliable analytical techniques and tools are indispensable in speciation analysis of trace elements, especially since environmental or biological samples are usually characterised by complex matrices. Here the methylating capability of hepatic cells was observed in vitro. HepG2 cells were incubated with colloidal bismuth subcitrate, bismuth cysteine and bismuth glutathione, respectively for a period of 24 h. For identification the cell lysate was ethylated by sodium tetraethyl borate under neutral conditions. After cryo focussing by purge and trap, the bismuth speciation was carried out viaGC/EI-MS/ICP-MS. Colloidal bismuth subcitrate and bismuth cysteine were methylated by HepG2 cells, while no methylated bismuth species was detected after incubation with bismuth glutathione.
It is already known that heavy metals in living organisms form complexes with sulfur containing molecules like cysteine and glutathione.13,14 Especially the formation of methylmercury cysteine in fish15 as a biologically active substance indicates the importance of small “biomolecules” for transport processes in animals. The formation of bismuth cysteine and bismuth glutathione complexes is also possible as shown by Burford and co-workers.16,17
Since alkylated bismuth compounds tend to decompose in water, the importance of bismuth cysteine complexes in the field of bismuth methylation is emphasized by a study proving the existence of methylbismuth cysteine in aqueous solution.18
Speciation analysis of bismuth compounds is usually carried out by liquid chromatography coupled to mass spectrometry17 as well as LT-GC/ICP-MS.10,19–22 For quantification of bismuth in alloys, stable volatile ethyl derivatives are used for quantitative determination of bismuth by ICP-AES.23 The very same technique has been used for volatilization and determination of methylated bismuth species in human blood samples after ingestion of bismuth(III) citrate–hydroxide complex.24
In the present qualitative study, the transformation of inorganic bismuth to methylated bismuth species by human hepatic cells (HepG2) is investigated by derivatization of these species with sodium tetraethyl borate. Then the volatile bismuth species are detected simultaneously by EI-MS and ICP-MS after gas chromatographic separation, revealing all bismuth containing compounds directly through correlation of both detector signals. This unique analytical system has recently proven its usefulness in the determination of volatile arsenic species generated by the (intestinal) microflora in human feaces.25
In a further step we isolated both complexes by adding small amounts of methanol until precipitation of a (slightly) yellow solid occurred. For isolation the crystalline product was subsequently filtered through a fibreglass filter. Finally the solid was dried in a vacuum desiccator containing silica gel for several days.
Colloidal bismuth subcitrate was prepared as follows: to a solution of bismuth citrate in aqueous ammonia (25%) and Dulbecco’s phosphate buffered saline (D-PBS, Gibco®, Invitrogen™, Karlsruhe, Germany) hydrochloric acid (37%, p.a., Riedel-de-Haën, Seelze, Germany) was added dropwise until a pH value of 8.5 was reached. The resulting CBS-suspension was used without further treatment and characterization.
The solution of each bismuth compound was prepared separately by dissolving CBS, bismuth cysteine or bismuth glutathione in D-PBS yielding a solution of 2000 mg bismuth compound per kg.
Two detection systems were used simultaneously: a 5973 N EI-MS (Agilent Technologies, Waldbronn, Germany), which served as a molecule selective detector for species conformation, and a 7500a ICP-MS (Agilent Technologies) for sensitive and element selective detection of the analytes. Working parameters for all instruments are listed in Table 1.
GC conditions | |
Column | DB-5 MS, 30 m 250 μm 25 μm |
Initial head pressure | 254.8 kPa |
Inlet Conditions PTV | |
Split | 1 : 50 |
Initial temperature | −100 °C for 10 min |
Heating rate | 800 °C/min |
Final temperature | 250 °C for 11 min |
Oven programme | |
Initial temperature | 60 °C |
Cooling rate 1 | −100 °C/min |
Final temperature 1 | 35 °C for 10 min |
Heating rate 2 | 30 °C/min |
Final temperature 2 | 230 °C for 10 min |
EI-MS parameters | |
Mass window | 200–400 amu |
Transfer line temperature | 280 °C |
MS Quad temperature | 150 °C |
Ionisation energy | 70 eV |
ICP-MS parameters | |
Argon flow | 15 l/min |
Carrier gas | 0.79 l/min |
Makeup gas | 0.23 l/min |
RF-Power | 1540 W |
Sampling depth | 5.0 mm |
Isotopes monitored (dwell time) | 203Tl, 205Tl, 209Bi (0.1 s) |
More details of this unique system are described elsewhere.27
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Fig. 1 (a) Ethylation of hepatic cells incubated with BiCys2 revealed two bismuth containing compounds b and d. (b) Using BiGSH2 for incubation instead, peak b does not occur in the chromatogram. |
A magnified view of the TIC (EI-MS) gave six representative peaks which were named as shown in Fig. 1. Taking the ICP-MS signal into account only peaks b and d represented bismuth compounds. All other peaks had their origin in siloxanes which could be derived from either the GC column or the Antifoam agent, which was added during sample preparation.
Peaks b and d were identified by interpreting the fragmentation of these compounds as shown in Fig. 2a and b.
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Fig. 2 (a) Fragmentation of peak b indicating diethyl methyl bismuth. (b) Fragmentation of peak d indicating triethyl bismuth. |
Consequently, these compounds could be identified as methylbismuth and an inorganic bismuth species forming diethyl methylbismuth and triethyl bismuth after derivatization by sodium tetraethyl borate.
Both chromatograms and mass spectra of bismuth cysteine and CBS, were identical. Peaks a, c, e and f could be identified as siloxanes D3, D4, D5 and D6 by matching the spectra with the NIST database. Considering the blank values (ESI,† Fig. S2 and Fig. S4) their origin is probably the added Antifoam agent.
In contrast, incubation with bismuth glutathione did not lead to the methylated bismuth compound as shown in Fig. 1b.
The methylbismuth species giving peak b did not occur in the chromatogram. For ethylation it is known that artifact formation can happen.28 So we observed carefully the formation of monomethyl species during ethylation of inorganic bismuth compounds.
As Fig. 3 demonstrates, no methylated species occurred during ethylation of the cell culture medium without hepatic cells containing bismuth compounds, nor did methylated bismuth species occur after adding sodium tetraethyl borate to a non-incubated sample of HepG2 in cell culture medium. For ethylation of bismuth it turned out to be important to buffer the solution to a pH value of about 7–8.
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Fig. 3 Ethylation of inorganic bismuth compounds in HEPES showing no methylated species. Peak b at tr = 11.564 min is not observed. |
Many independent studies have shown that TMBi is a volatile bismuth species detected in biological and environmental samples10,19 although the heat of formation ΔHf = 194 kJ/mol29 is rather high. This means permethylation of bismuth is an energetically inappropriate process. Nevertheless TMBi was detected, which can be explained by catalytically mediated biomethylation. Likewise, an exchange of methyl groups by monomethylated bismuth species as well as a stepwise methylation in the human liver is possible. An earlier work of Hirner and co-workers underlines the time shifted transfer of methyl groups to bismuth by monitoring the increase and decrease of methylated bismuth species in the blood of bismuth exposed probands.24
Nevertheless, further investigations have to be carried out to close the gap between mono- and trimethylated bismuth species both in vitro and in vivo.
One possible explanation of why bismuth cysteine enters the cells is that it uses amino acid carriers. Since bismuth glutathione is a possible excretion of HepG2, an intake into these cells is very unlikely. This mechanism is well studied for mercury cysteine by Clarkson et al.30 The intake of CBS can be explained by the possible formation of an amino acid complex of bismuth by amino acids that are part of the MEM solution.
Due to the lack of suitable bismuth standards and reference materials a quantitative reflection of the results is hardly reliable. Nevertheless, assuming that both methylated and non-methylated bismuth species have similar derivatization efficiencies, comparison of their resulting peak areas in ICP-MSchromatograms shows that approximately 2–3% of the inorganic bismuth species were methylated.
The values listed in Table 2 are given to make a rough estimate of bismuth conversion rates by hepatic cells. Since there are no reliable standards available for analytical balancing, we had to assume that there are no different effects on methylated and non-methylated bismuth compounds during derivatization and ionization, respectively.
Furthermore, to prevent the potential loss of volatile bismuth species, no gas was exchanged during incubation, although HepG2 cells require proper gas exchange for viability. So the methylation yields could also be limited by cell lifetime without oxygen.
Since this study was planned as a qualitative experiment only, we did not determine LODs. Typical LODs for volatile bismuth species on our analytical system are 0.1 to 0.3 ng per m3 of gaseous sample.10
Further investigations for clarification of which is the dominant species in the cells will be carried out. Likewise it has to be specified whether the monomethyl bismuth species were excreted by the cells or if they were still inside the cells and could only be detected because of the lysis. With respect to the analytics used, the sensitivity and ability of elemental detection of ICP-MS hyphenated to GC/EI-MS providing structural information is a powerful tool in bismuth speciation analysis.
The ICP-MS signal directly indicates where to look for bismuth-containing compounds. Otherwise an identification of bismuth compounds by EI-MS spectra alone would be more complicated, since bismuth is a monoisotopic element which subsequently has no characteristic isotope pattern. Moreover selected ion monitoring at m/z = 209 is not significant since this fragment is dominated by siloxane fragmentation originating from the Antifoam agent or from the GC column.
Besides this, we could show that hepatoma cells are able to methylate CBS and bismuth cysteine but not bismuth glutathione. These results show that human hepatoma cells have the potential to methylate bismuth and that the permethylated species is not generated within the observed period of time. If methylation was not observed as in the case of bismuth glutathione, this might result from the low uptake of this compound into the hepatoma cells. In conclusion, this study shows that bismuth is methylated by human hepatic cells in vitro. It appears from the result that both the intestinal microflora and the liver could be involved in bismuth biotransformation in the human body. In future studies, after suitable standards for method validation are available, further investigations concerning the transformation process and the cellular distribution of bismuth compounds should be done.
This work was financed by the German Research Foundation (Deutsche Forschungsgemeinschaft; DFG) “Synthesis and analysis of bismuth species with biological relevance” (“Synthese und Analyse biologisch relevanter Bismutspezies”).
Footnotes |
† Electronic supplementary information (ESI) available: Fig. S1–S5, EI-MS spectra. See DOI: 10.1039/b911945k |
‡ New correspondence address: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Reference Materials Unit, Retieseweg 111, B-2440 Geel, Belgium. Fax: +32 (0)14571548; Tel: +49 (0)14573005; E-mail: jens.boertz@uni-due.de |
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