Heinz
Langhals
*
Department of Chemistry, LMU University of Munich, Butenandtstr. 13, D-81377, Munich, Germany. E-mail: Langhals@lrz.uni-muenchen.de; Fax: +49 89-2180-77700; Tel: +49 89-2180-77699
First published on 6th December 2007
The dispersion of lipophilic perylene bisimides down to nanoparticle dimensions opens the aqueous phase to these highly fluorescent, water insoluble materials.
However, the application of such dyes in aqueous solution turned out to be appreciably more difficult. Strongly hydrophilic groups such as sulfonic acid groups,5 polyether groups,6 and pyridyl substituents,7 basically induce water solubility of 1; however, these materials exhibit a strong tendency to aggregate in the highly hydrophilic medium. Moreover, such dyes exhibit a high aptitude for the collection of heavy metal ions6 and this interferes with the preparation of the dyes in high purity.
A dispersion8 of a special derivative of 1 with a labile crystal lattice was only successful for low concentrations of particles and a spontaneous dispersion of a crown ether9 derivative of 1 is limited to this special dye. A direct application of the lipophilic, water-insoluble materials would bring about an appreciable improvement.
We have applied a dispersing detergent of maleinated linseed oil10 as an alternative; commercial name11 Bomol-4N. The lipophilic dyes 1 were dissolved in the dispersant and spread in more than a hundred times the quantity of water; for the preparation of nanoparticles by the application of detergents see ref. 12. Water-insoluble dyes were therefore required that are both lipophilic and readily soluble in the dispersant.
High solubility of 1 was attained by the attachment of long-chain secondary alkyl groups to the nitrogen atoms.3 Such dye preparations 1a form strongly yellow fluorescent liquids, remaining unchanged with storage, where the dispersion of the dye is so fine that it is not retained by a D5 glass filter, not even in traces; see Fig. 1. The porosity13 of D5 glass filers is 1.0–1.7 µm and particles can be retained that are smaller by a factor of three14 or even more. The complete passing of particles leaving the glass filter colourless indicates a size of the order of nanometres. The dispersion of the dye must have progressed far beyond the size of the previously reported8,9 particles because the latter could be completely collected by a D4 glass filter with pores of 9–15 µm size.
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Fig. 1 UV/Vis absorption and fluorescence spectra of 1. Left: absorption spectra from bottom to top: 1b in chloroform (red), 1b nanoparticles in water (black), 1a nanoparticle in water (blue). Right: fluorescence spectrum of 1b nanoparticles in water (black). |
The small size of the particles was verified by dynamic light scattering (DLS) measurements where a maximum of the distribution was found at 60 nm; see Fig. 2. The comparably narrow distribution is as remarkable as the result that no particles were found larger than 600 nm. Thus, complete dispersion down to nano-dimensions proceeded. Information about the direct environment of the nano-dispersed dye molecules was obtained by UV/Vis spectroscopy; the chromophore of 1 is very weakly, but characteristically, solvatochromic.15 Surprisingly, the UV/Vis spectra of nano-dispersed 1b in water are identical within 2 nm with the spectra in homogeneous solution in the lipophilic chloroform; see Fig. 1. The unaltered spectral shape indicates isolated chromophores in the nanoparticles where exciton interaction16 with neighbouring chromophores can be excluded. Such a type of nano-dispersion is very different from previously described microcrystalline particles with interacting chromophores; see for example ref. 17. Moreover, rather high concentrations of dyes in water can be obtained and are only limited by the solubility of the dye in the dispersant and the ratio between the dispersant and water. Flocculation does not occur, even not for the highest attainable dye concentrations. Appreciably coloured aqueous phases were obtained from 1a despite the rather short swallow-tail substituent. Even more deeply coloured phases can be obtained with the longer and more efficiently solubilizing substituents in 1b. The absorption of the dispersed dyes is overlaid by the absorption and light-scattering of the dispersant and the baseline of the spectrum deviates to higher values for shorter wavelengths; see Fig. 1. This deviation is strongest for 1a because its rather low solubility requires more dispersant than the more soluble dye 1b where matrix effects are essentially concentrated in the UV. Thus, 1b can be applied in the visible in the same way as a homogeneously dissolved dye.
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Fig. 2 Particle distribution of 1b nanoparticles in water: Intensity I of counts versus the size d of particles in nm. |
The pronounced fluorescence of the aqueous phases of 1 is remarkable. The fluorescence spectra of the aqueous phases of 1 resemble the spectra in chloroform; see Fig. 1. The fluorescence quantum yields of 1a and 1b are close to 100% as in solution in chloroform.4 The high fluorescence quantum yields are additionally indicators for isolated chromophores in the nanoparticles because interaction of chromophores such as in aggregates causes a weakening of fluorescence.18
Completely novel applications become possible for strongly lipophilic dyes which were otherwise inhibited by the insolubility in polar media. In particular, the high heat capacity of water allows applications with high radiation densities. On the other hand, the aqueous phase is of central importance for any use of dyes in biochemistry.
The dispersion of dyes demonstrated with perylene derivatives can be extended to other classes of dyes. Lipophilic and readily soluble dyes are therefore required. For example, indigo was substituted in the positions 5,5′ (2)19 and 6,6′ (3)20 with tert-butyl groups in order to increase the solubility.21 Saturated solutions in the dispersant were prepared with these dyes and spread in water as was described for 1. More dispersant than for 1 is necessary for 2 and 3, respectively, because of the lower solubility. Analogously to 1, coloured aqueous media were obtained with UV/Vis spectra resembling the spectra in lipophilic solvents; see Fig. 3. The nearly identical spectra of 2 in the dispersion and in lipophilic solvents are indicative of isolated chromophores in the particles. On the other hand, the spectra of 3 are slightly differently structured and this deviation may be taken as an indication of some aggregation of chromophores in the particles.
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Fig. 3 UV/Vis absorption spectra of 2 (blue) and 3 (black). Upper, thick lines: nano-dispersions in water; lower, thin lines: reference spectra in chloroform. |
The very similar results with the completely different chromophores 1, 2, and 3 indicate a broad application of the dispersion of dyes to form nanoparticles. The aqueous phase is of central importance for biochemical systems; thus, the novel concept opens up such applications for lipophilic dyes.
We thank Professor Dr. T. Bein for the particle measurements and Dr. H. Mottl from ISP Switzerland AG for a gift of Bomol-4N.
Footnote |
† Electronic supplementary information (ESI) available: Experimental details. See DOI: 10.1039/b717795j |
This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2008 |