Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 6, 2019
Previous Article Next Article

Surface acoustic waves enable rotational manipulation of Caenorhabditis elegans

Author affiliations

Abstract

Controllable, precise, and stable rotational manipulation of model organisms is valuable in many biomedical, bioengineering, and biophysics applications. We present an acoustofluidic chip capable of rotating Caenorhabditis elegans (C. elegans) in both static and continuous flow in a controllable manner. Rotational manipulation was achieved by exposing C. elegans to a surface acoustic wave (SAW) field that generated a vortex distribution inside a microchannel. By selectively activating interdigital transducers, we achieved bidirectional rotation of C. elegans, namely counterclockwise and clockwise, with on-demand switching of rotation direction in a single chip. In addition to continuous rotation, we also rotated C. elegans in a step-wise fashion with a step angle as small as 4° by pulsing the signal duration of SAW from a continuous signal to a pulsed signal down to 1.5 ms. Using this device, we have clearly imaged the dopaminergic neurons of C. elegans with pdat-1:GFP expression, as well as the vulval muscles and muscle fibers of the worm with myo-3::GFP fusion protein expression in different orientations and three dimensions. These achievements are difficult to realize through conventional (i.e., non-confocal) microscopy. The SAW manipulations did not detectably affect the health of the model organisms. With its precision, controllability, and simplicity in fabrication and operation, our acoustofluidic devices will be well-suited for model organism studies.

Graphical abstract: Surface acoustic waves enable rotational manipulation of Caenorhabditis elegans

Back to tab navigation

Supplementary files

Article information


Submitted
22 Sep 2018
Accepted
03 Feb 2019
First published
15 Feb 2019

Lab Chip, 2019,19, 984-992
Article type
Paper
Author version available

Surface acoustic waves enable rotational manipulation of Caenorhabditis elegans

J. Zhang, S. Yang, C. Chen, J. H. Hartman, P. Huang, L. Wang, Z. Tian, P. Zhang, D. Faulkenberry, J. N. Meyer and T. J. Huang, Lab Chip, 2019, 19, 984
DOI: 10.1039/C8LC01012A

Social activity

Search articles by author

Spotlight

Advertisements