Jump to main content
Jump to site search

Issue 1, 2015
Previous Article Next Article

In vivo fluorescence sensing of the salicylate-induced change of zinc ion concentration in the auditory cortex of rat brain

Author affiliations

Abstract

This study demonstrates a fluorescence method for in vivo sensing of the dynamic change of Zn2+ concentration in auditory cortex microdialysates induced by salicylate with N′-(7-nitro-2,1,3-benzoxadiazole-4-yl)-N,N,N′-tris(pyridine-2-ylmethyl) ethane-1,2-diamine (NBD-TPEA) as a probe. The excellent properties of the NBD-TPEA probe make it possible to achieve a high selectivity for Zn2+ sensing with the co-existence of amino acids and other metal ions as well as the species commonly existing in the cerebral system. To validate the method for in vivo fluorescence sensing of Zn2+ in the rat brain, we pre-mix the microdialysates in vivo sampled from the auditory cortex with the NBD-TPEA probe and then perfuse the mixtures into a fluorescent cuvette for continuous-flow fluorescence detection. The method demonstrated here shows a linear relationship between the signal output and Zn2+ concentration within the concentration range from 0.5 μM to 4 μM, with a detection limit of 156 nM (S/N = 3). The basal level of extracellular Zn2+ in auditory cortex microdialysates is determined to be 0.52 ± 0.082 μM (n = 4). This value is increased by the injection of 100 mg mL−1 of salicylate (1 μL min−1, 5 min, i.p.), reaches a peak at the time point of 90 min, and levels off with time. Such an increase is attenuated by the injection of MK-801, a potent and specific NMDA receptor antagonist, after the pre-injection of 100 mg mL−1 salicylate for 5 min. This study offers a fluorescence method for in vivo sensing of Zn2+ in the rat brain that could be useful for the investigations of chemical processes involved in brain functions.

Graphical abstract: In vivo fluorescence sensing of the salicylate-induced change of zinc ion concentration in the auditory cortex of rat brain

Back to tab navigation

Supplementary files

Article information


Submitted
06 Aug 2014
Accepted
12 Sep 2014
First published
12 Sep 2014

Analyst, 2015,140, 197-203
Article type
Paper
Author version available

In vivo fluorescence sensing of the salicylate-induced change of zinc ion concentration in the auditory cortex of rat brain

Q. Jiang, Z. Guo, Y. Zhao, F. Wang and L. Mao, Analyst, 2015, 140, 197
DOI: 10.1039/C4AN01443J

Social activity

Search articles by author

Spotlight

Advertisements