Cardiovascular biodiagnosis by infrared spectroscopy through choline determination

Abstract

In this work, a green analytical method has been proposed for the diagnosis of heart disease. In this method, infrared spectroscopy has been employed for quantitative determination of choline as an important correlated biochemical in blood samples. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy has been used for analysis of blood serum samples while the spectrometric data were processed by using partial least squares (PLS). In the experimental step, 82 blood serum samples were studied in the 4000–600 cm⁻¹ spectral region. Preprocessing methods such as standard normal variate (SNV) and multiplicative scatter correction (MSC) were utilized with no evidence of the analytical output, while orthogonal signal correction (OSC) could affect the accuracy of the method severely. The RMSEP for the OSC-PLS model was 0.39%; thus it could be considered as an appropriate data processing strategy for the ATR-FTIR spectrometric determination of choline in serum blood samples. It is rapid, reliable, non-destructive and free of sample preparation or chemical reagent consumption and is called as a green diagnostic approach.