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Issue 12, 2015
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Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

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Abstract

We report the development of photo-crosslinked siRNA strands modified at each terminus with p-cyanostilbene. The siRNA was nuclease resistant and retained RNAi activity. We further studied the activation mechanism of the covalently-crosslinked siRNA. Interestingly Dicer, which is known to generate siRNA with overhanging 3′ ends from the precursor siRNA, did not cleave the crosslinked siRNA at all. Our results suggest that the activation of the crosslinked siRNAs required cleavage by Argonaute2.

Graphical abstract: Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

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Publication details

The article was received on 07 Jul 2015, accepted on 23 Oct 2015 and first published on 03 Nov 2015


Article type: Communication
DOI: 10.1039/C5BM00231A
Author version available: Download Author version (PDF)
Citation: Biomater. Sci., 2015,3, 1534-1538
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    Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

    Y. Kamiya, K. Iishiba, T. Doi, K. Tsuda, H. Kashida and H. Asanuma, Biomater. Sci., 2015, 3, 1534
    DOI: 10.1039/C5BM00231A

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