Jump to main content
Jump to site search

Issue 12, 2015
Previous Article Next Article

Live cell tracking of symmetry break in actin cytoskeleton triggered by abrupt changes in micromechanical environments

Author affiliations

Abstract

With the aid of stimulus-responsive hydrogel substrates composed of ABA triblock copolymer micelles, we monitored the morphological dynamics of myoblast (C2C12) cells in response to an abrupt change in the substrate elasticity by live cell imaging. The remodeling of actin cytoskeletons could be monitored by means of transient transfection with LifeAct-GFP. Dynamic changes in the orientational order of actin filaments were characterized by an order parameter, which enables one to generalize the mechanically induced actin cytoskeletons as a break of symmetry. The critical role that acto-myosin complexes play in the morphological transition was verified by the treatment of cells with myosin II inhibitor (blebbistatin) and the fluorescence localization of focal adhesion contacts. Such dynamically tunable hydrogels can be utilized as in vitro cellular micro-environments that can exert time-dependent stimuli to mechanically regulate target cells.

Graphical abstract: Live cell tracking of symmetry break in actin cytoskeleton triggered by abrupt changes in micromechanical environments

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 Jun 2015, accepted on 26 Aug 2015 and first published on 08 Sep 2015


Article type: Paper
DOI: 10.1039/C5BM00205B
Citation: Biomater. Sci., 2015,3, 1539-1544
  • Open access: Creative Commons BY license
  •   Request permissions

    Live cell tracking of symmetry break in actin cytoskeleton triggered by abrupt changes in micromechanical environments

    S. Inoue, V. Frank, M. Hörning, S. Kaufmann, H. Y. Yoshikawa, J. P. Madsen, A. L. Lewis, S. P. Armes and M. Tanaka, Biomater. Sci., 2015, 3, 1539
    DOI: 10.1039/C5BM00205B

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements