Issue 13, 2015

Inhibitors of thermally induced burn incidents – characterization by microbiological procedure, electrophoresis, SEM, DSC and IR spectroscopy

Abstract

Differential scanning calorimetry (DSC) and thermogravimetric (TGA) investigations, acetate electrophoresis (CAE), Fourier-transform infrared spectrometry (FTIR), scanning electron microscopy (SEM) analysis and microbiological procedures were all carried out after heating the samples to a temperature sufficient for simulating a burn incident. In particular, the purpose of the present study was to analyze the effect of antioxidants, such as fucoidan from brown seaweed and flame-retardant cyclic organophosphates and phosphonates, on an organic chicken skin that gets changed by a burn incident. DSC was considered to be a useful tool in assessing in vitro temperature-mediated cross-linking; an innovative analytical conclusion was obtained from the experimentation described in the paper. FTIR tests revealed that heating a dry organic chicken skin to the boiling point leads to the disappearance of a wide band in the 1650–1550 cm−1 area or the conversion of a band, which may be attributed to the intermolecular β-sheet aggregates. Fucoidan from brown seaweed and flame-retardant cyclic organophosphates and phosphonates probably bind with the collagen that is changed by the burn (in addition to the influence of antioxidant solutions on samples of a blank or not boiled organic chicken skin) incident forming a polymer film with the collagen of the chicken skin surface (SEM analysis), decreasing the aggregation process and native collagen recovery. Good bacteriostatic properties were determined for fucoidan samples from brown seaweed and flame-retardant cyclic organophosphates and phosphonates against the pathogenic bacteria Escherichia coli and Staphylococcus aureus. Thus, it was observed that the fucoidan incorporated into collagen films can be used as a therapeutically active biomaterial that speeds up the wound-healing process.

Graphical abstract: Inhibitors of thermally induced burn incidents – characterization by microbiological procedure, electrophoresis, SEM, DSC and IR spectroscopy

Article information

Article type
Paper
Submitted
18 二月 2015
Accepted
11 五月 2015
First published
15 五月 2015

Analyst, 2015,140, 4599-4607

Inhibitors of thermally induced burn incidents – characterization by microbiological procedure, electrophoresis, SEM, DSC and IR spectroscopy

A. Pielesz, A. Machnicka, A. Gawłowski, J. Fabia, E. Sarna and W. Biniaś, Analyst, 2015, 140, 4599 DOI: 10.1039/C5AN00329F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements