Issue 13, 2015

Light up ClO in live cells using an aza-coumarin based fluorescent probe with fast response and high sensitivity

Abstract

Hypochlorous acid (HClO)/hypochlorite (ClO), one of the reactive oxygen species (ROS), is a key microbicidal agent used for natural defense; however, HClO is also responsible for some human diseases. Although much effort has been made to develop HClO-selective fluorescent probes, many of them display a delayed response time and nanomole-sensitive probes are rare. In this study, we designed and synthesized an aza-coumarin based fluorescent probe AC-ClO for ClO determination with fast response (completed within 2 min) and high sensitivity (detection limit is 25 nM). AC-ClO displayed a color change from pink to light yellow and a remarkable “turn-on” fluorescence response towards ClO. Confocal fluorescence microscopy experiments demonstrated that the probe could be applied for the live-cell imaging of exogenous and endogenous ClO.

Graphical abstract: Light up ClO− in live cells using an aza-coumarin based fluorescent probe with fast response and high sensitivity

Supplementary files

Article information

Article type
Paper
Submitted
21 Apr 2015
Accepted
01 May 2015
First published
08 May 2015

Analyst, 2015,140, 4594-4598

Author version available

Light up ClO in live cells using an aza-coumarin based fluorescent probe with fast response and high sensitivity

J. Fan, H. Mu, H. Zhu, J. Wang and X. Peng, Analyst, 2015, 140, 4594 DOI: 10.1039/C5AN00777A

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