Biosynthesis of the polyketide mellein from CD3CO2H and 13CD3CO2H in Aspergillus melleus: detection of deuterium by 2H n.m.r. and edited 13C n.m.r. spectra

Abstract

The pattern of incorporation of deuterium into mellein from CD₃CO₂H and ¹³CD₃CO₂H was investigated by ²H n.m.r. and edited ¹³C n.m.r. spectra; the deuterium incorporated into the C-4 methylene group was found to be evenly distributed between the two diastereoisotopic positions and molecules were detected which retained two deuteriums at that site.