Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes

Ran Liu; Ying He; Tian Lan; Jingjing Zhang

doi:10.1039/D1AN00008J

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes†

Ran Liu,

‡^a Ying He,‡^a Tian Lan^b and Jingjing Zhang

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China
E-mail: jing15209791@nju.edu.cn

^b GlucoSentient, Inc. 2100 S. Oak Street, Suite 101, Champaign, IL 61820, USA

Abstract

Integrating CRISPR–Cas12a sensors with a portable glucose meter (PGM) was developed based on the target-induced activation of the collateral cleavage activity of Cas12a. Considering the portability, low cost and facile incorporation of the PGM system with suitable Cas12a sensors to recognize many targets, the CRISPR/Cas12a-PGM system demonstrated here paves a way to further broaden the POC applications of CRISPR-based diagnostics.

This article is part of the themed collections: Analyst Recent HOT articles and Celebrating 100 years of Chemistry at Nanjing University

Download options Please wait...

Supplementary files

Supplementary information PDF (2606K)

Article information

DOI: https://doi.org/10.1039/D1AN00008J
Article type: Communication
Submitted: 03 Jan 2021
Accepted: 24 Mac 2021
First published: 25 Mac 2021

Download Citation

Analyst, 2021,146, 3114-3120

Permissions

Request permissions

Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes

R. Liu, Y. He, T. Lan and J. Zhang, Analyst, 2021, 146, 3114 DOI: 10.1039/D1AN00008J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Analyst

Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes

Social activity

Search articles by author

Spotlight

Advertisements