Issue 24, 2016

Surface modification with E-cadherin fusion protein for mesenchymal stem cell culture

Abstract

To effectively expand human mesenchymal stem cells (hMSCs) in vitro without affecting their innate biological properties, a fusion protein (hE-cad-Fc) consisting of a human E-cadherin extracellular domain and an immunoglobulin G Fc region was fabricated and used as a biomimetic matrix for MSC culture surface modification. The results showed that cells cultured on hE-cad-Fc-modified polystyrene surfaces exhibited improved proliferation and paracrine functions compared with cells cultured on unmodified and collagen-modified polystyrene surfaces. Meanwhile, surfaces modified with hE-cad-Fc effectively inhibited cell apoptosis even under the serum deprivation conditions. Additionally, the hE-cad-Fc not only up-regulated the expression of β-catenin in MSCs and stimulated the cellular membrane complex of E-cadherin/β-catenin, but also effectively activated the intracellular signals such as EGFR, AKT and ERK phosphorylation. Therefore, hE-cad-Fc appeared to be a promising candidate for biological surface modification and stem cell culture.

Graphical abstract: Surface modification with E-cadherin fusion protein for mesenchymal stem cell culture

Article information

Article type
Paper
Submitted
25 mar 2016
Accepted
19 maj 2016
First published
20 maj 2016

J. Mater. Chem. B, 2016,4, 4267-4277

Surface modification with E-cadherin fusion protein for mesenchymal stem cell culture

Y. Zhang, H. Mao, M. Qian, F. Hu, L. Cao, K. Xu, Q. Shuai, C. Gao, R. Lang, T. Akaike and J. Yang, J. Mater. Chem. B, 2016, 4, 4267 DOI: 10.1039/C6TB00765A

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