Issue 66, 2024

Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds

Abstract

A VMYH motif was determined to help ketosynthases in polyketide assembly lines select α,β-unsaturated intermediates from an equilibrium mediated by an upstream dehydratase. Alterations of this motif decreased ketosynthase selectivity within a model tetraketide synthase, most significantly when replaced by the TNGQ motif of ketosynthases that accept D-β-hydroxy intermediates.

Graphical abstract: Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds

Supplementary files

Article information

Article type
Communication
Submitted
21 jun 2024
Accepted
18 jul 2024
First published
19 jul 2024
This article is Open Access
Creative Commons BY license

Chem. Commun., 2024,60, 8712-8715

Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds

K. A. Ray, N. Saif and A. T. Keatinge-Clay, Chem. Commun., 2024, 60, 8712 DOI: 10.1039/D4CC03034F

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