Issue 43, 2018

Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice

Abstract

Hypochlorous acid (HClO) is a promising diagnostic marker for inflammation and relevant diseases. Although many probes were previously developed for HClO imaging, the development of organ targeting probes is still lacking. Herein, we designed and synthesized a series of cyanine derivatives as ratiometric fluorescent probes to detect endogenous HClO in the lungs with inflammation. By installing diverse lipid chains and amino groups on cyanine, we identified that ClO1, with one n-octadecane chain and two 2-[[2-(dimethylamino)ethyl]methylamino]-ethyl groups, is a superior probe to target the lungs over other major organs in mice. ClO1 was able to sense both exogenous and endogenous HClO in A549 (human lung epithelial) cells through fluorescence ratiometric imaging. In a lipopolysaccharide (LPS)-induced lung inflammation mouse model, ClO1 effectively captured endogenous HClO in the lungs after intravenous administration. Overall, these cyanine-derived probes merit further development as organ targeting HClO sensors.

Graphical abstract: Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice

Supplementary files

Article information

Article type
Edge Article
Submitted
20 jul 2018
Accepted
04 set 2018
First published
05 set 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2018,9, 8207-8212

Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice

X. Zhang, W. Zhao, B. Li, W. Li, C. Zhang, X. Hou, J. Jiang and Y. Dong, Chem. Sci., 2018, 9, 8207 DOI: 10.1039/C8SC03226B

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