Issue 27, 2021

Fluorescent detection of target proteins via a molecularly imprinted hydrogel

Abstract

Proteins are typically separated by an immune reaction, such as an enzyme-linked immunosorbent assay, and are detected by selective fluorescent labeling. This has potential for complicated procedures and the denaturation of proteins by labeling, and is cost consuming. In this study, we propose a technique for the selective separation and detection of a target protein using a molecularly imprinted hydrogel (PI gel) with fluorescent monomers. We focused on 8-anilino-1-naphthalenesulfonic acid (ANS), where the fluorescence intensity is easily changed by the interaction with proteins, and successfully synthesized the ANS monomer and a poly(ethylene glycol) (PEG) conjugated ANS monomer. The PI gel with the ANS monomers using bovine serum albumin (BSA) as a template showed the selective adsorption of BSA and the fluorescence intensity increased due to the adsorption of BSA.

Graphical abstract: Fluorescent detection of target proteins via a molecularly imprinted hydrogel

Supplementary files

Article information

Article type
Paper
Submitted
26 des 2020
Accepted
02 jun 2021
First published
04 jun 2021

Anal. Methods, 2021,13, 3086-3091

Fluorescent detection of target proteins via a molecularly imprinted hydrogel

T. Kubo, N. Watanabe, S. Ikari, C. Liu, E. Kanao, T. Naito, T. Sano and K. Otsuka, Anal. Methods, 2021, 13, 3086 DOI: 10.1039/D0AY02341H

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